Summary
A study of surface remodeling activity and osteocyte lacunar area was made in young and adult rats maintained on a low-calcium diet, to explore cellular mechanisms of bone resorption. The diet produced active remodeling of the endosteal part of the femoral cortex, with a decrease in the amount of bone present. Surface resorption, with numerous osteoclasts, was evident, but there was no evidence of osteocytic osteolysis in bone which, by tetracycline labeling, could be identified as existing at the commencement of the experimental period. Osteocyte lacunae in bone formed during the period of calcium deprivation were somewhat larger than lacunae in control animals, apparently because of interference with the formation or maturation of the perilacunar tissue.
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References
Kölliker A (1873) Die Normale Resorption des Knochengewebes. Vogel, Leipzig
Gaillard PG (1961) Parathyroid and bone in tissue culture. In: Greep RO, Talmage TV (eds) The parathyroids. Charles C Thomas, Springfield, p 20
Hancox NM, Boothroyd B (1963) Structure-function relationships in the osteoclast. In: Soggnaes RF (ed) Mechanisms of hard tissue destruction. Publication no. 75 for sthe American Association for the Advancement of Science, Washington, DC, p 497
Gonzales F, Karnovsky MJ (1961) Electron microscopy of osteoclasts in healing fractures of rat bone. J Biophys Biochem Cytol 9:299–316
Scott BL, Pease DC (1956) Electron microscopy of the epiphyseal apparatus. Anat Record 126:465–495
Göthlin G, Ericsson JL (1971) Fine structural localization of acid phosphomonoesterase in the brush border region of osteoclasts. Histochemie 28:337–344
Holtrop ME, King GJ (1977) The ultrastructure of the osteoclast and its functional implications. Clin Orthop 123:177–196
Bonucci E (1981) New knowledge on the origin, function and fate of osteoclasts. Clin Orthop 158:252–269
Bélanger LF, Robichon J, Migicovsky BB, Copp DH, Vincent J (1963) Resorption without osteoclasts (osteolysis). In: Soggnaes RF (ed) Mechanisms of hard tissue destruction. Publication no. 75 of the American Association for the Advancement of Science, Washington, DC, p 531
Bélanger LF (1965) Osteolysis: an outlook on its mechanisms and causation. In: Gaillard PG, Talmage TV, Budy AM (eds) The parathyroid glands: ultrastructure, secretion and function. University of Chicago, p 137
Bélanger LF (1969) Osteocytic osteolysis. Calcif Tissue Res 4:1–12
Virchow R (1871) Die cellularpathologie. Hirschwald, Berlin
von Recklinghausen F (1910) Untersuchungen ueber Rachitis und Osteomalacie. Fischer, Jena
Duriez J, Cauchoix J (1967) Le role des ostéocytes dans la résorption du tissu osseux. Presse méd 75:1297–1302
Baylink DJ, Wergedal JE (1971) Bone formation by osteocytes. Am J Physiol 221:669–678
Whalen JP, Winchester P, Krook L, Dische R, Nunez E (1971) Mechanisms of bone resorption in human metaphyseal remodelling. Am J Roentgenol 112:526–531
Baylink DJ, Sipe J, Wergedal J, Whittemore OJ (1973) Vitamin D-enhanced osteocytic and osteoclastic bone resorption. Am J Physiol 224:1345–1357
Bernard J, Meunier P (1975) L'analyse morphométrique et l'ostéolyse périostéocytaire: son application au diagnostic des hyperparathyroidies. Ann d'Anat Pathol 20:367–380
Krempien B, Ritz E (1978) Effects of parathyroid hormone on osteocytes. Ultrastructural evidence of anisotropic osteolysis and involvement of the cytoskeleton. Metab Bone Dis and Rel Res 1:55–65.
Baud CA (1968) Submicroscopic structure and functional aspects of the osteocyte. Clin Orthop 56:227–236
Jande SS (1972) Effects of parathormone on osteocytes and their surrounding bone matrix: an electron microscopic study. Z Zellforsch 130:463–470
Tonna EA (1972) Electron microscopic evidence of alternating osteocytic osteoclastic and osteoplastic activity in the perilacunar walls of aging mice. Connective Tissue Res 1:221–230
Baud CA, Boivin G (1978) Effects of hormones on osteocyte function and perilacunar wall structure. Clin Orthop 136:270–281
Parfitt AM (1977) The cellular basis of bone turnover and bone loss. Clin Orthop 127:236–247
Marotti G, Ledda M, Del Rio N, Fadda M (1977) Quantitative analysis of osteolytic versus osteoclastic activity in various experimental osteoporoses. 1. Disuse osteoporosis. Calcif Tissue Res 22(suppl):242–246
Marotti G (1979) Osteocyte orientation in human lamellar bone and its relevance to the morphometry of periosteocytic lacunae. Metab Bone Dis and Rel Res 1:325–333
Boyde A (1980) Evidence against osteocytic osteolysis. Metab Bone Dis et Rel Res 25:239–255
Muglia MA, Volpi G, Remaggi F, Cane V, Palazzini S, Zaffe D, Marotti G (1982) Activity of osteoclasts and osteocytes in compact human bone at various ages, both with and without osteoporosis. Ital J Orthop & Traumatol 8:117–125
Rasmussen H, Bordier P (1974) The physiological and cellular basis of metabolic bone disease. Williams and Wilkins, Baltimore.
Harrison M, Fraser R (1960) Bone structure and metabolism in calcium deficient rats. J Endocrinol 21:197–205
Gershon-Cohen J, McClendon JF, Jowsey J, Foster WC (1962) Osteoporosis produced and cured in rats by low and high calcium diets. Radiology 78:251–252
Salomon CD (1972) Osteoporosis following calcium deficiency in rats. Calcif Tissue Res 8:320–333
de Winter FR, Steendijk R (1975) The effect of a low calcium diet in lactating rats. Observations on the rapid development and repair of osteoporosis. Calcif Tissue Res 17:303–316
Rasmussen P (1977) Calcium deficiency, pregnancy and lactation in rats. Some effects on blood chemistry and the skeleton. Calcif Tissue Res 23:87–94
Rasmussen P (1977) Calcium deficiency, pregnancy and lactation in rats. Microscopic and microradiographic observations on bones. Calcif Tissue Res 23:95–102
Salomon CD, Volpin G (1970) Fine structure of bone resorption in experimental osteoporosis caused by calciumdeficient diet in rats. An electron microscopic study of compact bone. Calcif Tissue Res 4 (suppl):80–82
Ornoy A, Wolinsky I, Guggenheim K (1974) Structure of long bones of rats and mice fed a low calcium diet. Calcif Tissue Res 15:71–76
Lok E, Jaworski ZFG (1976) Changes in the periosteocytic lacunae size observed under the experimental conditions in the adult dog. In: Jaworski ZFG (ed) Proceedings of the first workshop on bone morphometry, University of Ottawa Press, p 297–300
Wergedal J (1969) Enzymes of protein and phosphate catabolism in rat bone. Calcif Tissue Res 3:55–56
Te Velde J, Burkhardt R, Kliverda K, Leenheers-Binnendijk L, Sommerfeld W (1977) Methyl-methacrylate as an embedding medium in histopathology. Histopathology 1:319–330
Freiman DG (1954) Organic chelating agent in demineralization of bone for histochemical study of alkaline phosphatase. Am J Clin Pathol 24:227–231
Higuchi S, Suga M, Dannenberg AM, Schofield BH (1979) Histochemical demonstration of enzyme activities in plastic-and paraffin-embedded tissue sections. Stain Technol 54:5–12
Bennett HS, Wyrick AD, Lee SW, McNeil JA (1976) Science and art in preparing tissues embedded in plastic for light microscopy, with special reference to glycol methacrylate, glass knives and simple stains. Stain Technol 51:71–97
Warmke HE, Lee SLF (1976) Improved staining procedures for semithin epoxy sections of plant tissues. Stain Technol 51:179–185
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Sissons, H.A., Kelman, G.J. & Marotti, G. Mechanisms of bone resorption in calcium-deficient rats. Calcif Tissue Int 36, 711–721 (1984). https://doi.org/10.1007/BF02405394
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DOI: https://doi.org/10.1007/BF02405394