Skip to main content
Springer Nature Link
Log in

Cytogenetic studies in mammalian cells after treatment with insect pathogenic viruses [Baculoviridae]. I.In vivo studies with rodents

  • Published:
Entomophaga Aims and scope Submit manuscript

Abstract

No consistent deleterious effects directly attributable to the nuclear polyhedrosis virus ofMamestra brassicae (L.),Autographa californica (Speyer) and the granulosis virus ofLaspeyresia pomonella (L.) were observed, when bone marrow cells of Chinese hamsters and NMRI mice were examined for chromosomal aberrations or sister chromatic exchanges. The animals were exposed to thein vivo andin vitro produced viruses acutely or chronicaly (90 resp. 97 days) by oral gavage or intraperitoneal injection. The treated animals neither showed signs of health disturbances nor abnormal behaviour nor changes in body weight in comparison to the controls.

Résumé

Il n'y a pas d'anomalies (aberrations et échanges de chromatides) dans les chromosomes des cellules de la moelle osseuse des hamsters chinois et des souris NMRI après l'application des virus de polyédroses nucléaires deMamestra brassicae (L.),Autographa californica (Speyer) et la granulose deLaspeyresia pomonella (L.). Les animaux ont reçu les virus repliquésin vivo etin vitro en doses aiguës et chroniques (respectivement 90 et 97 j.) par application orale et intrapéritonéale. En comparaison des animaux témoins, les animaux traités n'ont pas manifesté d'irrégularités en ce qui concerne leurs poids, santé ou réactions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Allen, J.W., Schuler, C.F. &Latt, S.A. — 1978. Bromodeoxyuridine tablet methodology forin vivo studies of DNA synthesis. —Somat. Cell Genet., 4, 393–405.

    Article  CAS  PubMed  Google Scholar 

  • Banowetz, G.M., Fryer, J.L.., Iwai, P.J. & Martignoni, M.E. — 1976. Effects of the Douglas-fir tussock moth nucleopolyhedrosis virus (baculovirus) on three species of salmonid fish. —USDA Forest Research Paper PNW-214.

  • Bartsch, H.D. — 1970. Virus-induced chromosomal alterations in mammals and man. In: Chemical Mutagenesis in Mammals and Man. (F. Vogel &G. Röhrborn eds.). —Springer Verlag, Berlin-Heidelberg, 420–432.

    Google Scholar 

  • Beek Van Der, C.P. — 1980. On the origin of the polyhedral protein of the nuclear polyhedrosis virus ofAutographa californica. —Mededelingen Landbouwhogeschool Wageningen, Nederland, 80–5 (1980), pp. 2–4.

    Google Scholar 

  • Brown, R.L. &Crossen, P.E. — 1976. Increased incidence of sister chromatid exchanges in Rauscher leukaemia virus infected mouse embryo fibroblasts. —Exp. Cell Res., 103, 418–420.

    Article  CAS  PubMed  Google Scholar 

  • Burges, H.D., Croizier, G. &Huber, J. — 1980. A review of safety tests on baculoviruses. —Entomophaga, 25, 329–340.

    Google Scholar 

  • Couch, J.A. — 1974. An enzootic nuclear polyhedrosis virus of pink shrimp: Ultrastructure, prevalence, and enhancement. —J. Invertebr. Pathol., 24, 311–332.

    CAS  PubMed  Google Scholar 

  • Derbyshire, J.B. — 1974. Mammalian toxicity of polyhedrosis virus prior to registration as a viral insecticide for the control of the spruce budworm — cytopathogenicity in mammalian cells. — Report to the Insect Pathology research Institute, Sault Ste-Marie, Ontario, Canada.

  • Falcon, L.A. — 1978. Economical and biological importance of baculoviruses as alternatives to chemical pesticides. In: Safety Aspects of Baculoviruses as Biological Insecticides. (H.G. Miltenburger ed.). —Bundesministerium für Forschung & Technologie, Bonn, 27–46.

  • Gröner, A. — 1976. Das Kernpolyedervirus der Kohleule (Mamestra b.): Seine Produktion und Erproburg für die biologische Schädlingsbekämpfung. —Z. Angew. Entomol., 82, 138–143.

    Google Scholar 

  • — — 1978. Studies on the specifity of the nuclear polyhedrosis virus ofMamestra brassicae (L.) [Lep.: Noctuidae]. In: Safety Aspects of Baculoviruses as Biological Insecticides. (H.G. Miltenburger ed.). —Bundesministerium für Forschung & Technologie, Bonn, 265–269.

    Google Scholar 

  • Gröner, A., Huber, J. &Krieg, A. — 1978. Untersuchungen mit Baculoviren an Säugetieren. —Z. Angew. Zool., 65, 69–80.

    Google Scholar 

  • Harnden, D.G. — 1974. Virus, chromosomes, and tumors: The interaction between viruses and chromosomes. In: Chromosomes and Cancer. (J. German ed.). —John Wiley & Sons. New York, London, Sydney, Toronto, 151–190.

    Google Scholar 

  • Heimpel, A.M. &Buchanan, L.K. — 1967. Human feeding tests using a nuclear polyhedrosis virus ofHeliothis zea. —J. Invertebr. Pathol., 9, 55–57.

    CAS  PubMed  Google Scholar 

  • Himeno, M., Sakai, F., Onodera, K., Nakai, H., Fukada, T. &Kawade, Y. — 1967. Formation of nuclear polyhedral bodies and nuclear polyhedrosis virus of silkworm in mammalian cells infected with viral DNA. —Virology, 33, 507–512.

    Article  CAS  PubMed  Google Scholar 

  • Huber, J. — 1978. About the host spectrum of the codling moth granulosis virus. In: Safety Aspects of Baculoviruses as Biological Insecticides. (H.G. Miltenburger ed.). —Bundesministerium für Forschung & Technologie, Bonn, 75–80.

    Google Scholar 

  • Huber, J. — 1980. 7 Jahre Freiland-Versuche mit dem Granulosevirus des Apfelwicklers in der Bundesrepublik Deutschland. —Proc. Int. Symp. IOBC/WPRS “Integrierter Pflanzenschutz in der Land- und Forstwirtschaft” (Wien, 8-12 Okt. 1979), 583.

  • Ignoffo, C.M. &Rafajko, R.R. — 1972.In vitro attempts to infect primate cells with the nucleopolyhedrosis virus ofHeliothis. —J. Invertebr. Pathol., 20, 321–325.

    CAS  PubMed  Google Scholar 

  • Ignoffo, C.M. — 1973. Effects of entomopathogens on vertebrates. —Ann. NY. Acad. Sci., 217, 141–172.

    CAS  PubMed  Google Scholar 

  • Kawanishi, C.Y. — 1978. Review of safety tests and methods of evaluating infectivity. In: Viral Pesticides: Present Knowledge and Potential Effects on Public and Environmental Health. (M.D. Summers & C.Y. Kawanishi eds.). —Research Triangle Park, North Carolina: Health Effects Research Laboratory, Office of Health and Ecological Effects, U.S. Environmental Protection Agency. EPA-600/978-026, 199–210.

  • Knuutila, S., Mäki-Paakkanen, J. &Hokkanen, E. — 1978. An increased frequency of chromosomal changes and SCEs in cultured blood lymphocytes of 12 subjects vaccinated against small pox. —Hum. Genet., 41, 89–96.

    Article  CAS  PubMed  Google Scholar 

  • Kurvink, K., Bloomfield, C.D. &Cervenka, J. — 1978. Sister chromatid exchange in patients with viral disease. —Exp. Cell. Res., 113, 450–452.

    Article  CAS  PubMed  Google Scholar 

  • Latt, S.A., Schreck, R.R., Loveday, K.S. &Schuler, C.F. — 1979.In vitro andin vivo analysis of sister chromatid exchange. —Pharmacol. Rev., 30, 501–535.

    Google Scholar 

  • McIntosh, A.H. &Maramorosch, K. — 1973. Retention of insect virus infectivity in mammalian cell cultures. —N. Y. Entomol. Soc., 81, 175–182.

    Google Scholar 

  • McIntosh, A.H. &Shamy, R. — 1975. Effects of the nuclear polyhedrosis virus (NPV) ofAutographa californica on a vertebrate viper cell line. —Ann. N.Y. Acad. Sci., 266, 327–331.

    Google Scholar 

  • — — 1980. Biological studies of a baculovirus in a mammalian cell line. —Intervirology, 13, 331–341.

    CAS  PubMed  Google Scholar 

  • Miltenburger, H.G. — 1978. No effect of NPV on mammalian cellsin vivo andin vitro (cell proliferation, chromosome structure). In: Safety Aspects of Baculoviruses as Biological Insecticides. (H.G. Miltenburger ed.). —Bundesministerium für Forschung & Technologie, Bonn, 185–200.

    Google Scholar 

  • — — 1980. Viral pesticides: Hazard evaluation for non-target organisms and safety testing. —Ecol. Bull. (Stockholm), 31, 57–74.

    Google Scholar 

  • Miltenburger, H.G. &Reimann, R. — 1980. Viral pesticides: Biohazard evaluation on the cytogenetic level. —Develop. Biol. Standard., 46, 217–222.

    CAS  Google Scholar 

  • Nichols, W.W., Bradt, C.I., Tou, L.H., Goodley, M. &Segawa, M. — 1978. Induction of sister chromatid exchanges by transformation with simian virus 40. —Cancer Res., 38, 960–964.

    CAS  PubMed  Google Scholar 

  • Perry, P. &Wolff, S. — 1974. New Giemsa method for the differential staining of sister chromatids. —Nature (Lond.), 251, 156–158.

    Article  CAS  Google Scholar 

  • Reed, L.J. &Muench, H. — 1938. A simple method of estimating fifty per cent end points. —Am J. Hyg., 27, 493–497.

    Google Scholar 

  • Schmid, W., Arakaki, D.T., Breslau, N.A. &Culbertson, J.C. — 1970. Chemical mutagenesis, the Chinese hamster bone marrow as anin vivo test system. —Humangenetik, 11, 103–118.

    Google Scholar 

  • Stich, H.F. &Yohn, D.S. — 1970. Viruses and Chromosomes. —Progr. Med. Virol., 12, 78–127.

    Google Scholar 

  • Summers, M.D., Engler, R., Falcon, A. &Vail, P.V. (eds.). — 1975. “Baculoviruses for Insect Pest Control: Safety Considerations”. —John Wiley & Sons, New York, London, Sydney, Toronto.

    Google Scholar 

  • Whitehouse, H.L.K. — 1974. Chromosome integration of viral DNA: The open-replicon hypothesis of carcinogenesis. In: Chromosomes and Cancer. (J. German ed.). —John Wiley & Sons, New York, London, Sydney, Toronto, 41–76.

    Google Scholar 

  • World Health Organization. — 1973a. The use of viruses for the control of insect pests and disease vectors. —WHO Tech. Rep., Ser. 531.

Download references

Author information

Authors and Affiliations

  1. Institute of Zoology, Cell Biology Laboratory, Technical University, D-6100, Darmstadt, Federal Republic of Germany

    R. Reimann & H. G. Miltenburger

Authors
  1. R. Reimann
    View author publications

    You can also search for this author inPubMed Google Scholar

  2. H. G. Miltenburger
    View author publications

    You can also search for this author inPubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Reimann, R., Miltenburger, H.G. Cytogenetic studies in mammalian cells after treatment with insect pathogenic viruses [Baculoviridae]. I.In vivo studies with rodents. Entomophaga 27, 267–276 (1982). https://doi.org/10.1007/BF02374810

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02374810

Keywords