Summary
The aim of this work was to compare the performance of the MSD, ECD and NPD systems when used for drug assay in biological fluids. As a practical test, six non steroidal anti-inflammatory drugs added to plasma samples were detected and quantified. The analyses were carried out after solvent extraction from an acidic medium and subsequent methylation. The linearity of response was tested for all the detection systems in the range of 1 to 25 ng/ml. Precision and accuracy were determined at 1, 5 and 10 ng/ml. The minimum quantifiable level for the six drugs was about 1 ng/ml with each of the three detection systems.
Similar content being viewed by others
References
J. E. Lovelock, Anal. Chem.35, 474 (1963).
J. E. Lovelock, J. Chromatogr.1, 35 (1958).
L. Giuffrida, J. Ass. Off. Agric. Chem.47, 293 (1964).
W. A. Aue, C. W. Gehrke, R. C. Tindle, D. L. Standle, C. D. Ruyle, J. Gas Chromatogr.4, 42 (1967).
C. Giachetti, S. Canali, G. Zanolo, J. Chromatogr.279, 587 (1983).
C. Giachetti, G. Zanolo, P. Poletti, F. Perovanni, HRC13, 792 (1989).
G. R. Verga, J. Chromatogr.279, 657 (1983).
R. J. Tallarida, R. B. Murray, Manual of Pharmacologic Calculations with Computer Programs, Springer-Verlag, New York, 2nd ed., 1987, Procedure 5, p. 16.
R. J. Tallarida, R. B. Murray, Manual of Pharmacologic Calculations with Computer Programs, Springer-Verlag, New York, 2nd ed., 1987, Procedure 34, p. 113.
S. Eskborg, Clin. Chem.27, 1311 (1981).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Giachetti, C., Assandri, A., Zanolo, G. et al. MSD, ECD and NPD performances compared in the assay of six non steroidal anti-inflammatory drugs in plasma samples. Chromatographia 39, 162–169 (1994). https://doi.org/10.1007/BF02274495
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02274495