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HPLC determination of antioxidant synergists and ascorbic acid in some fatty pharmaceuticals, cosmetics and food

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Summary

A specific and sensitive reverse-phase HPLC method for the quantitative determination of ascorbic acid and antioxidant synergists (1-tartaric acid, citric acid, lactic acid as lithium lactate and EDTA) in fatty pharmaceuticals, cosmetics and food has been developed. Two extraction procedures were used; treatment with hot water, and extraction with water from a hexane dilution of the product. No significant differences between the two procedures were found (p<0.05), except for ascorbic acid. Quantitative determinations were performed using a C-18 column and sulfuric acid (pH 1.95) mobile phase. With detection, at 210 nm, lactic acid overlapped with ascorbic acid, but the former could be readily identified by TLC. Ascorbic acid was detected at 254 nm, when lactic acid (as lithium lactate) did not interfere in the analysis. Mean recoveries for tartaric, citric and lactic acids were in the range 96–101%.

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References

  1. B.N. Stuckey, “Handbook of food additives”, 2nd ed., T. Furia, ed., CRC Press Inc., Cleveland, 1972, p. 185–231

    Google Scholar 

  2. J.R. Hart, Cosmet. Toilet.98, 54–58 (1983)

    Google Scholar 

  3. J.E. Reynolds, “Martindale, The Extra Pharmacopeia”, 29th ed., The Pharmaceutical Press, London, 1989

    Google Scholar 

  4. United States Pharmacopeial Convection. United States Pharmacopeia XXII, The National Formulary XVII, Rockville, 1990

  5. D.M. Johnson, L.C. Gu, in “Encyclopedia of Pharmaceutical Technology”, Vol. 1, J. Swarbrick and J.C. Boyland eds., Marcel Dekker Inc., New York, 1988, p. 415–449

    Google Scholar 

  6. J.M. Nikitakis, “CTFA Cosmetic Ingredient Handbook”, 1st ed., The Cosmetic Toiletry and Fragance Association Inc., Washington, 1988

    Google Scholar 

  7. D. Tusseau, C. Benoit, J. Chromatog.395, 323–333 (1987)

    Google Scholar 

  8. E. Mentasti, M. Gennaro, C. Sarzanini, J. Chromatog.323, 177–189 (1985)

    Google Scholar 

  9. S. Ashoor, J. Welty, J. Chromatog.287, 917–920 (1984)

    Google Scholar 

  10. R.T. Marsili, H. Ostapenko, J. Food Sci.46, 52–57 (1981)

    Google Scholar 

  11. L.G. Long, J.D. Winefordner, Anal. Chem.55 (7), 712A-724A (1983)

    Google Scholar 

  12. J. Dittmann, J. Chromatog.34, 410–417 (1968)

    Google Scholar 

  13. Pharmacopoea Helvetica VII. Office Central Fédéral des Imprimés et du Matériel, Berne, 1987

  14. “Lactic acid in Butterfat” in “Official Methods of Analysis of the Association of Official Analytical Chemists”, 14th ed, S. Williams ed., 1984

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Authors and Affiliations

  1. Departmento de Farmacia y Tecnologia Farmaceutica, Universidad de Navarra, 31080, Pamplona, Spain

    J. M. Irache, I. Ezpeleta & F. A. Vega

Authors
  1. J. M. Irache
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  2. I. Ezpeleta
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  3. F. A. Vega
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Irache, J.M., Ezpeleta, I. & Vega, F.A. HPLC determination of antioxidant synergists and ascorbic acid in some fatty pharmaceuticals, cosmetics and food. Chromatographia 35, 232–236 (1993). https://doi.org/10.1007/BF02269708

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  • DOI: https://doi.org/10.1007/BF02269708

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