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The inhibitory effect of trilinolein on norepinephrine-induced β-myosin heavy chain promoter activity, reactive oxygen species generation, and extracellular signal-regulated kinase phosphorylation in neonatal rat cardiomyocytes

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Journal of Biomedical Science

Abstract

The myocardial protective effects of trilinolein, isolated from the traditional Chinese herbSanchi (Panax notoginseng), are thought to be related to its antioxidant activity. However, the intracellular mechanism underlying the protective effect of trilinolein in the heart remains unclear. In the present study, we investigated the effect of trilinolein on norepinephrine (NE)-induced protein synthesis in cardiomyocytes. Cultured neonatal rat cardiomyocytes were stimulated with NE, then protein content, [3H]-leucine incorporation, and β-myosin heavy chain (β-MyHC) promoter activity were examined. The effect of trilinolein on NE-induced intracellular reactive oxygen species (ROS) generation was measured with a redoxsensitive fluorescent dye (2′,7′-dichlorofluorescin diacetate) and extracellular signal-regulated kinase (ERK) phosphorylation by Western blotting. Trilinolein inhibited NE-increased protein synthesis, β-MyHC promoter activity, and intracellular ROS. Both trilinolein and the antioxidant, N-acetyl-cysteine, decreased NE- and H2O2-induced protein synthesis, β-MyHC promoter activity, and ERK phosphorylation. These data indicate that trilinolein inhibits NE-induced protein synthesis via attenuation of ROS generation in cardiomyocytes.

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Authors and Affiliations

  1. Graduate Institute of Medical Sciences, Taipei Medical University, Taiwan (ROC)

    Ju-Chi Liu & Paul Chan

  2. Graduate Institute of Biomedical Technology, Taipei Medical University, Taiwan (ROC)

    Horng-Mo Lee

  3. Institute of Biomedical Sciences, Academia Sinica, Taiwan (ROC)

    Jin-Jer Chen, Neng-Lang Shih, Yen-Ling Chen &  Tzu-Hurng Cheng

  4. Department of Internal Medicine, National Taiwan University Hospital and National Taiwan University College of Medicine, Taiwan (ROC)

    Jin-Jer Chen

  5. Department of Pharmacology, National Defense Medical Center, Taipei, Taiwan (ROC)

    Tzu-Hurng Cheng

  6. School of Chinese Medicine, China Medical College, Taichung, Taiwan, ROC

    Hong-Jye Hong

  7. Department of Medicine, Taipei Medical University-Wan Fang Hospital, No. 111 Hsing-Lung Road, Sec. 3, 117, Wen Shan District Taipei, Taiwan (ROC)

    Ju-Chi Liu, Paul Chan, Wen-Sen Lee &  Tzu-Hurng Cheng

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Liu, JC., Chan, P., Chen, JJ. et al. The inhibitory effect of trilinolein on norepinephrine-induced β-myosin heavy chain promoter activity, reactive oxygen species generation, and extracellular signal-regulated kinase phosphorylation in neonatal rat cardiomyocytes. J Biomed Sci 11, 11–18 (2004). https://doi.org/10.1007/BF02256544

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  • DOI: https://doi.org/10.1007/BF02256544

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