Abstract
Metabolite profiling and DNA microarray analysis of global gene expression profiles were employed to characterize the bioactivities of the herbal extract ofAnoectochilus formosanus (AF), a popular folk medicine with anticancer activity, in MCF-7 cancer cells. The pharmacogenomic activities of this plant extract as a crude phyto-compound mixture were compared to those conferred by the single-compound drug, plumbagin. A similar level of complexity in transcriptional regulation at the genomic level was observed for both AF extract- and plumbagin-treated MCF-7 cells, as revealed by the number of up- or downregulated genes as well as by the specific but distinct patterns found in the gene-clustering analysis. This finding offers evidence to support the search for fractionated medicinal herb extracts or phytocompound mixtures, in addition to single-compound drugs, as defined therapeutic agents.
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References
Celis JE, Kruhoffer M, Gromova I, Frederiksen C, Ostergarrd M, Thykuaer T, Gropmov P, Yu J, Palsdottir H, Magnusson N, Orntoft T. Gene expression profiling: Monitoring transcription and translation products using DNA microarrays and proteomics. FEBS Lett 480:2–16;2000.
Jaiswal AS, Bloom LB, Narayan S. Long-patch base excision repair of apurinic/apyrimidinic site DNA is decreased in mouse embryonic fibroblast cell lines treated with plumbagin: Involvement of cyclin-dependent kinase inhibitor p21Waf-1/Cip-1. Oncogene 21:5912–5922;2002.
Koop CE. The future of medicine. Science 295:233;2002.
Lau WK, Chiu SK, Ma JT, Tzeng CM. Linear amplification of catalyzed reporter deposition technology on nylon membrane microarray. Biotechniques 33:564–570;2002.
Wang H, Mao Y, Chen AY, Zhou N, LaVoie EJ, Liu LF. Stimulation of topoisomerase II-mediated DNA damage via a mechanism involving protein thiolation. Biochemistry 40:3316–3323;2001.
Wang SY, Kuo YH, Chang HN, Tsay HS, Lin KF, Yang NS, Shyur LF. Profiling and characterization of antioxidant activities inAnoectochilus formosanus Hayata. J Agric Food Chem 50:1859–1865;2002.
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Yang, NS., Shyur, LF., Chen, CH. et al. Medicinal herb extract and a single-compound drug confer similar complex pharmacogenomic activities in MCF-7 cells. J Biomed Sci 11, 418–422 (2004). https://doi.org/10.1007/BF02254447
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DOI: https://doi.org/10.1007/BF02254447