Abstract
The adhesion molecule, lymphocyte function associated antigen 1 (LFA-1) consisting of two subunits, CD11a and CD18, mediates lymphocyte migration into tissue and cell effector functions. Previous observations showed no differences in LFA-1 expression by circulating lymphocytes between inflammatory bowel disease patients and controls. The aim of the present work was to study subsets of circulating LFA-14 lymphocytes in ulcerative colitis (UC) patients versus healthy controls. Peripheral blood mononuclear cells were obtained from 16 UC patients and 10 healthy volunteers. The percentages of CD11alo, CD11ahi; CD18lo, CD18hi T and B cells, as well as CD25 expression on these cells were studied using double staining with monoclonal antibodies and panning procedures. The percentage of CD11hi and CD18hi T cells was significantly decreased in quiescent UC patients as compared to active disease patients and healthy controls (P<0.05). The majority of CD25+ T cells were expressing CD11a and CD18 with low density. A detectable percentage, 2% (range 1–6%), of CD11ahiCD25+ (but not CD18hiCD25+) was found in UC patients with moderate to severe disease, but not in those with inactive UC or healthy controls. In conclusion, the percentage of CD11ahi and CD18hi T cells is decreased in peripheral blood of quiescent UC patients, which is probably associated with the effect of specific treatment. The percentage of CD11ahi+ T cells is increased in peripheral blood of patients with active (moderate and severe) UC, which most likely reflects a sustained T-cell activation due to a persistent inflammatory process.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Lo SK, Van Seventer GA, Levin SM, Wright SD: Two leukocyte receptors (CD11a/CD18 and CD11b/CD18) mediate transient adhesion to endothelium by binding to different ligands. J Immunol 143:3325–3329, 1989
Cronstein BN, Weismann G: The adhesion molecules of inflammation. Arthritis Rheum 36:147–157, 1993
Springer AT: Adhesion receptors of the immune system.Nature 346:425–434, 1990
Issekutz TB: Inhibition of lymphocyte endothelial adhesion andin vivo lymphocyte migration to cutaneous inflammation by TA-3, a new monoclonal antibody to rat LFA-1. J Immunol 149:3394–3402, 1992
Whitcup SM, De Barge LR, Caspi RR, Harning R, Nussenblatt RB, Chan CC: Monoclonal antibodies against ICAM-1 (CD54) and LFA-1 (CD11a/CD18) inhibit experimental autoimmune uveitis. Clin Immunol Immunopathol 67:143–150, 1993
Sadahiro M, Allen MD: Monoclonal antibody against LFA-1 (CD18) inhibits cellular and vascular rejection in rabbit cardiac allografts. Nippon Kyobu Geka Gakkai Zasshi 42:1148–1153, 1994
Kawasaki K, Yaoita E, Yamamoto T, Tamatani T, Miyasaka M, Kihara I: Antibodies against intracellular adhesion molecule-1 and lymphocyte function-associated antigen-1 prevent glomerular injury in rat experimental crescentic glomerulonephritis. J Immunol 150:1074–1083, 1993
Pardi R, Bender JR, Dettori C, Giannazza E, Engleman EG: Heterogenous distribution and transmembrane signaling properties of lymphocyte function-associated antigen (LFA-1) in human lymphocyte subsets. J Immunol 143:3157–3166, 1989
Richardson B, Powers D, Hooper F, Yung RL, O'Rourke K: Lymphocyte function-associated antigen-1 overexpression and T cell autoreactivity. Arthritis Rheum 37:1363–1372, 1994
Moingeon PE, Lucich JL, Stebbins CC, Recny MA, Wallner BP, Koyasu S, Reinherz EL: Complementary roles for CD2 and LFA-1 adhesion pathways during T cell activation. Eur J Immunol 21:605–610, 1991
David V, Leca G, Corvaia N, Le Deist F, Boumsell L, Bensussan A: Proliferation of resting lymphocytes is induced by triggering T cells through an epitope common to the three CD18/CD11 leukocyte adhesion molecules. Cell Immunol 136:519–524, 1991
Koopman G, de Graaff M, Huysmans AC, Meijer CJ, Pals ST: Lymphocyte function-associated antigen-1 alpha (CD11a): Differential effects on resting and activated T cells. Eur J Immunol 22:1851–1856, 1992
Vyth-Dreese FA, Dellemijn TA, Frijhoff A, van Kooyk Y, Figdor CG: Role of LFA-1/ICAM-1 in interleukin-2-stimulated lymphocyte proliferation. Eur J Immunol 23:3292–3299, 1993
Langholz E, Munkholm P, Nielsen OH, Kreiner S, Binder V: Incidence and prevalence of ulcerative colitis in the county of Copenhagen. Scand J Gastroenterol 26:1247–1256, 1991
Binder V: A comparison between clinical state, macroscopic and microscopic appearances of rectal mucosa, and cytologic picture of mucosal exudate in ulcerative colitis. Scand J Gastroenterol 5:627–632, 1970
Hamblin A, Taylor M, Bernhagen J, Shakoor Z, Mayall S, Noble G, McCarthy D: A method of preparing blood leucocytes for flow cytometry which prevents upregulation of leucocyte integrins. J Immunol Methods 146:219–228, 1992
Wysocki LJ, Sato VL: “Panning” for lymphocytes: A method for cell separation. Proc Natl Acad Sci USA 75:2844–2848, 1978
Greenfield SM, Hamblin A, Punchard NA, Thompson RPH: Expression of adhesion molecules on circulating leucocytes in patients with inflammatory bowel disease. Clin Sci 83:221–226, 1992
Nakamura S, Ohtani H, Watanabe Y, Fukushima K, Matsumoto T, Kitano A, Kobayashi K, Nagura H:In situ expression of the cell adhesion molecules in inflammatory bowel disease. Evidence of immunologic activation of vascular endothelial cells. Lab Invest 69:77–85, 1993
Desroches CV, Rigal D, Andreoni C: Regulation and functional involvement of distinct determinants of leucocyte function-associated antigen 1 (LFA-1) in T cell activationin vitro. Scand J Immunol 33:177–186, 1991
Author information
Authors and Affiliations
Additional information
The project was supported by grants from direktor Jacob Madsen's and Hustru Olga Madsen's Foundation.
Rights and permissions
About this article
Cite this article
Kirman, I., Nielsen, O.H. LFA-1 subunit expression in ulcerative colitis patients. Digest Dis Sci 41, 670–676 (1996). https://doi.org/10.1007/BF02213121
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02213121