Abstract
Purpose
The male factor is nowadays one of the major problems in the treatment of infertility. New methods of assisted fertilization such as the intracytoplasmic sperm injection (ICSI) show better fertilization and pregnancy rates than classical IVF.
Method
In this study, we present a new technique of sperm preparation: the “mini-swim-up.”
Conclusion
This technique, used in conjunction with the ICSI procedure, improves pregnancy and fertility rates in cases of severe oligoasthenoteratozoospermia.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Uehara T, Yanagimachi R: Microsurgical injection of spermatozoa into hamster eggs with subsequent transformation of sperm nuclei into male pronuclei. Biol Reprod 1976;15: 467–470
Lanzendorf SE, Maloney MK, Veeck LL, Slusser J, Hodgen GC, Rosenvaks Z: A preclinical evaluation of pronuclear formation by microinjection of human spermatozoa into human oocytes. Fertil Steril 1988;49:835–842
Palermo G, Joris H, Devroey P, Van Steirteghem AC: Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet 1992;340:17–18
Joris H, Nagy Z, Liu J, Bocken G, Vankelecom A, Van Ranst H, Desmet B, Devroey B, Van Steirteghem AC: Intracytoplasmic sperm injection. Hum Reprod 1994;Suppl 4(Abstracts),9:17
Bonduelle M, Legein J, Buysse A, Devroey B, Van Steirteghem AC, Libaers I: Comparative follow-up study of 130 children born after IVF. Hum Reprod 1994;Suppl 4(Abstracts),9:38
Nagy ZP, Liu J, Joris H, Bocken G, Tournaye H, Devoroey P, Van Steirteghem AC: Extremely impaired Semen parameters and the outcome of intracytoplasmic sperm injection. Hum Reprod 1994;Suppl 4(Abstracts),9:27–29
Tournaye H, Janssens G, Verheyen P, Devroey P, Van Steirteghem AC: In vitro fertilization in couples with previous fertilization failure using sperm incubated with pentoxyfilline and 2-desoxyadenosine. Fertil Steril 1994;61 (Suppl);6:35
Tournaye H, Devroey P, Liu J, Nagy Z, Lissens W, Van Steirteghem AC: Microsurgical epididymal sperm aspiration and intracytoplasmic sperm injection: a new effective approach to infertility as a result of congenital bilateral abscence of the vas. Fertil Steril 1994;61;6:1045–1051
Anguiano A, Oates RD, Amos JA, Dean M, Gerrard A, Steward C: Congenital bilateral abscence of the vas deferens: a primary genetic form of cystic fibrosis. JAMA 1992;267:1794–1797
WHO-Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interaction, 3rd ed. Berlin, Heidelberg, New York, Springer, 1993
Kruger TF, Acosta AA, Simmons KF, Swanson RJ, Matta JF, Oehninger S: Predictive value of abnormal sperm morphology in in vitro fertilization. Fertil Steril 1988;49:112–117
Ombelet W, Fourie F leR, Vandeput H, Bosnians E, Cox A, Janssen M, Kruger T: Teratozoospermia and in-vitro fertilization: a randomized prospective study. Hum Reprod 1994;9:1479–1484
Kruger TF, Menkveld R, Stander FSH, Lombard CJ, Van der Merwe JP, Van Zyl JA, Smith K: Sperm morphologic features as a prognostic factor in in vitro fertilization. Fertil Steril 1986;46:1118–1123
Van Steirteghem AC, Liu J, Joris H, Nagy Z, Janssenswillen C, Tournaye H, Derde M-P, Van Assche E, Devroey P: Higher success rate by intracytoplasmic sperm injection than by subzonal insemination. A report of a second series of 300 consecutive treatment cycles. Hum Reprod 1993;8: 1055–1060
Al-Hasani S, Trotnow S, Sadtler C, Hahn J: In vitro fertilization and embryo transfer of pre-ovulatory rabbit oocytes. Eur J Obstet Gynecol Reprod Biol 1986;21:187–195
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Al Hasani, S., Küpker, W., Baschat, A.A. et al. Mini-swim-up: A new technique of sperm preparation for intracytoplasmic sperm injection. J Assist Reprod Genet 12, 428–433 (1995). https://doi.org/10.1007/BF02211143
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02211143