Abstract
The cyanobacteriumSpirulina platensis is one of the most promising sources of the polyunsaturated fatty acid γ-linolenic acid (GLA). The GLA content ofSpirulina can be enhanced by cultivation under light-dark cycles in the laboratory or outdoors. Thus, in strain BP, the GLA content increased from 1.2 to 1.6% when cultivated under light-dark cycles. Moreover, in the derived mutant Z19, the GLA content reached 2.4% when cultivated outdoors. To the best of our knowledge, this is the highest GLA content ever reported for any alga.
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Biagi PL, Bordoni A, Masi M, Ricci G, Fanelli C, Patrizi A, Ceccolini E (1988) Evening primrose oil (Efamol) in the treatment of children with atopic eczema. Drugs exptl Clin. Res. 14: 291–297.
Bligh EG, Dyer WJ (1959) A rapid method for total lipid extraction and purification. Can. J. Biochem. Physiol. 37: 911–917.
Ciferri O (1983)Spirulina, the edible microorganism. Microbiol. Rev. 47: 551–578.
Cohen Z, Vonshak A, Richmond A (1987) Fatty acid composition ofSpirulina strains grown under various environmental conditions. Phytochem. 26: 2255–2258.
Cohen Z, Cohen S (1991) Preparation of eicosapentaenoic acid concentrate fromPorphyridium cruentum. J. Am. Oil Chem. Soc. 68: 16–19.
Cohen Z, Didi S, Heimer YM (1992) Over-production of γ-linolenic and eicosapentaenoic acids by algae. Plant Phys. 98: 569–572.
Cohen Z, Reungjitchachawali M, Siangdung W, Tanticharoen M (1993a) Production and partial purification of γ-linolenic acid and some pigments fromSpirulina platensis. J. appl. Phyc. 5: 109–115.
Cohen Z, Norman NH, Heimer YM (1993b) Evaluating the potential of substituted pyridazinones for inducing polyunsaturated fatty acid overproduction in algae. Phytochem. 32: 259–264.
Cohen Z, Reungjitchachawali M, Siangdung W, Tanticharoen M, Heimer YM (1993c) Herbicide resistant lines of microalgae: Growth and fatty acid composition. Phytochem. 34: 973–978.
Hirano M, Mori H, Miura Y, Matsunaga N, Nakamura N, Matsunaga T (1990) γ-linolenic acid production by microalgae. Appl. Biochem. Biotechnol. 24: 183–191.
Horrobin DF (1983) The role of essential fatty acids and prostaglandins in the premenstrual syndrome. J. Reprod. Med. 28: 465–468.
Hudson BJF, Karis IG (1974) The lipids of the algaSpirulina. J. Sci. Food Agric. 25: 759–763.
Ishikawa T, Fujiyama Y, Igarashi C, Morino M, Fada N, Kagami A, Sakamoto T, Nagano M, Nakamura H (1989) Clinical features of familial hypercholesterolemia. Atherosclerosis 75: 95–103.
Kenyon CN, Stanier RY (1970) Possible evolutionary significance of polyunsaturated fatty acids in blue-green algae. Nature 227: 1164–1166.
Mori T, Muranaka T, Miki W, Yamaguchi K, Konosu S, Watanabe T (1987) Pigmentation of cultured sweet smelt fed diets supplemented with a blue-green algaSpirulina maxima. Nippon Suisan Gakkaishi 53: 433–438.
Murata N, Ishida I (1987) Lipids of blue-green algae (cyanobacteria). In P. Stumpf (ed.), The Biochemistry of Plants, Vol. 9. Academic Press, New York: 315–347
Nakahara T, Yokochi T, Kamisaka Y, Suzuki O (1992) Gamma linolenic acid from genusMortierella. In Kyle DJ, Ratledge C (eds), Industrial Application of Single Cell Oil. American Oil Chemists' Society, Champaign), IL; 61–97.
Nichols BW, Wood BJB (1968) The occurrence and biosynthesis of γ-linolenic acid in a blue-green alga,Spirulina platensis. Lipids 3: 46–50.
Roughan PG (1989)Spirulina: a source of dietary gamma-linolenic acid? J. Sci. Food Agric. 47: 85–93.
Suzuki O, Toshihiro Y (1985) Microbial production of γ-linolenic acid-rich phosphatidyl choline. Jpn Kokai Tokkyo Koho JP 62 25, 989
Sukenik A, Carmeli Y (1990) Lipid synthesis and fatty acid composition inNannochloropsis sp. (Eustimatophyceae) grown in a light-dark cycle. J. Phycol. 26: 463–469.
Tanticharoen M, Bunnag B, Vonshak A. (1993) Cultivation ofSpirulina using secondary treated starch wastewater. Australian Biotechnology 3: 223–336.
Vonshak A (1986) Laboratory techniques for the cultivation of microalgae. In Richmond A (ed.), Handbook of Microalgal Mass Culture. CRC Press, Boca Raton, FL: 117–145.
Vonshak A (1987) Biological limitations in developing the biotechnology for algal mass cultivation. Sciences de l'Eau 6: 99–103.
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Tanticharoen, M., Reungjitchachawali, M., Boonag, B. et al. Optimization of γ-linolenic acid (GLA) production inSpirulina platensis . J Appl Phycol 6, 295–300 (1994). https://doi.org/10.1007/BF02181942
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DOI: https://doi.org/10.1007/BF02181942