Abstract
An improved filter paper disk bioassay for determining activities of plant growth regulators was developed and evaluated. Indole-3-acetic acid (IAA), (±)-abscisic acid (ABA), and 6-furfurylamino-purine (kinetin) were dissolved in 95% ethanol at fixed dilutions. Specific concentrations of each growth regulator were then evenly dropped onto individual 6-cm paper disks and the solvent evaporated. The activities of the above three water-insoluble plant growth regulators in both solution and the disk assay were compared using the excised cucumber (Cucumis sativus L.) cotyledon root formation bioassay (IAA), wheat (Triticum aestivum L.) coleoptile straight growth bioassay (ABA), and cucumber cotyledon expansion bioassay (kinetin), and similar results were obtained at each concentration. The possible principle of this method has been studied using the cucumber cotyledon expansion bioassay. The results suggested that the disks, as carriers, had highly dispersed kinetin molecules on them and greatly accelerated the dissolution and diffusion of kinetin from disks to water.
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References
Anderson DR, Camper ND (1987) Application of the disk method: responses to growth regulators. J Plant Growth Regul 6:57–65
Barry AL, Garcia F, Thrupp LD (1970) An improved single-disk method for testing the antibiotic susceptibility of rapidly growing pathogens. Am J Clin Pathol 53:149–158
Bauer AW, Kirby WMM, Sherris JC, Turck M (1966) Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45:493–496
Chen SQ (1984) Report on a special visit concerning triacontanol. Plant Physiol Commun 1:73–75
Chen WL, Song YR, Tsui C (1979) Comparative study on the bioassay between N6, O2″-dibutyryl cyclic adenosine 3,5-monophosphate and cytokinin. Acta Bot Sin 21:263–268
Hartley D, Kidd H (1983) The agrochemicals handbook. The Royal Society of Chemistry, The University Nottingham, England, pp A111, A232
Li GR, Zhao ZR, Zheng QL, Shi YN, Zhang CX, Chen RY (1984) The active substances inhibiting the elongation of wheat coleoptiles fromSophora alopecuroides L. Chinese Bull Bot 2:20–22
Meudt WJ, Bennett HW (1978) Rapid bioassay for auxin. Physiol Plant 44:422–428
Nitsch JP, Nitsch C (1956) Studies on the growth of coleoptile and first internode sections. A new sensitive straight-growth test for auxins. Plant Physiol 31:94–111
Rao VS, Braun JW, Khan AA (1976) Promotive effects of organic solvents and kinetin on dark germination of lettuce seeds. Plant Physiol 57:446–449
Worthing CR (1983) The pesticide manual. The British Crop Protection Council, p 301
Xu BM, Gu ZH (1987) The use of organic solvents to enhance seed vigor. Plant Physiol Commun 4:17–20
Zhao ZR (1990) The effects of some phenolics on the IAA oxidase activity of excised cucumber cotyledons and wheat coleoptile segments. Acta Scientiarum Naturalium Universitatis Nan Kaiensis 4:75–79
Zhao ZR, Huang GQ (1990) A new root-formation test. Acta Bot Sin 32:815–818
Zhao ZR, Li GR, Huang GQ (1991) Promotive effect of chrysanthemic acids on adventitious root formation in some plants. J Plant Growth Regul 10:221–223
Zhao ZR, Wu ZL, Zheng QL (1988) The use of “filter paper method” in determining plant hormones. Chinese Bull Bot 5:57–59A0443017 00005 CS-SPJRNPDF [HEADSUP]
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Zhao, Z.R., Wu, Z.L., Huang, G.Q. et al. An improved disk bioassay for determining activities of plant growth regulators. J Plant Growth Regul 11, 209–213 (1992). https://doi.org/10.1007/BF02115479
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DOI: https://doi.org/10.1007/BF02115479