Abstract
Purpose: Two types of glass wool were used to remove leukocytes in semen for evaluation of reactive oxygen species production by spermatozoa in oligozoospermic patients with leukocytospermia.
Methods: Semen samples were prepared using fine-structure glass wool (SpermFertil) and coarse-structure glass wool. In each treatment group, native semen was evaluated for sperm concentration, percentage motility, viability, leukocyte concentration, and production of reactive oxygen species.
Results: Electron microscopically, SpermFertil showed a higher number of leukocytes attached to the fibers compared to coarse-structure glass wool. Leukocytes in native semen and after glass wool filtration as determined by peroxidase cytochemistry confirmed this observation. Reactive oxygen species decreased from 45.303 counts/107 viable cells in native semen to 15.806 counts/107 cells in coarse structure wool and 7.465 counts/107 cells in Spermfertil, respectively.
Conclusions: Removal of leukocytes from semen of oligozoospermic patients by means of glass wool filtration is a useful method to distinguish production of reactive oxygen species by leukocytes versus sperm cells.
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Sánchez, R., Concha, M., Ichikawa, T. et al. Glass wool filtration reduces reactive oxygen species by elimination of leukocytes in oligozoospermic patients with leukocytospermia. J Assist Reprod Genet 13, 489–494 (1996). https://doi.org/10.1007/BF02066530
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DOI: https://doi.org/10.1007/BF02066530