Summary
Molecular biology allowed considerable improvements in diagnostic procedures by production of new and more specific sonds for the detection of traces of viruses, both on the nucleic acid and protein levels, and by determining the immune response of the host to specific antigens. Improvements in sensitivity and improved correlation to the stage of viral disease are already evident from several applications and strongly suggest a broad application of these approaches.
Similar content being viewed by others
References
Arrand, J.E., Preparation of single-stranded cDNA probes, in: Nucleic Acid Hybridisation — a Practical Approach, pp. 20–21. Eds B.D. Hames and S.J. Higgins. IRL Press, Oxford-Washington DC 1985.
Burns, J., Chan, V.T.W., Jonasson, J.A., Fleming, K.A., Taylor, S., and McGee, O.D., Sensitive system for visualising biotinylated DNA probes hybridised in situ: rapid sex determination of intact cells. J. clin. Path.58 (1985) 1085–1092.
Chou, P.Y., and Fasman, G.D., Prediction of the secondary structure of proteins from their amino acid sequence. Adv. Enzym.47 (1978) 45–148.
Dürst, M., Schwarz, E., and Gissmann, L., Integration and persistence of human papillomavirus DNA in genital tumors, in: Viral Etiology of Cervical Cancer, pp.273–280. Eds R. Peto and H. zur Hausen. Banbury Report 21. Cold Spring Harbor Laboratory 1986.
Falser, N., Bandtlow, I., Haus, M., and Wolf, H., Detection of PRV-DNA in the inner ear of intranasally infected BALB-C mice with nucleic acid hybridization in situ. J. Virol.57 (1986) 335–339.
Falser, N., Bandtlow, I., Rziha, A., Bauer, G., Pöppel, S., Haus, M., and Wolf, H., Reactivation of PRV infection of swine: A model for sudden deafness of man? Zbl. Bakt. Mikrobiol. Hyg, in press.
Feinberg, A.P., and Vogelstein, B., A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Analyt. Biochem.132 (1983) 6–13.
Forster, A.C., McInnes, J.L., Skingle, D.C., and Symons, R.H., Non-radioactive hybridization probes by the chemical labelling of DNA and RNA with a novel reagent, photobiotin. Nucl. Acids Res.13 (1985) 745–761.
Garnier, J., Osguthorpe, O.J., and Robson, B., Analysis of the accuracy and implication of simple methods for predicting the secondary structure of globular proteins. J. molec. Biol.120 (1978) 97–120.
Gissmann, L., and Schneider, A., Diagnosis of papillomaviurs infection by nucleic acid hybridization, in: Rapid Methods and Automation in Microbiology and Immunology, pp. 43–47. Ed. K.-O. Habermehl. Springer, Berlin-Heidelberg-New York-Tokyo 1985.
Kubanek, B., Ernst, P., Schäfer, U., Ostendorf, P., and Wolf, H., A controlled trial of intravenous hyperimmuneglobin in the prevention of cytomegalovirus infection in bone marrow transplant recipients. Exp. Hemat.12 (1984) 111–112.
Kwok, S., Mack, D.H., Mullis, K.B., Poiesz, B., Ehrlich, G., Blair, D., Friedman-Kien, A., and Skninsky, J.J., Identification of human immunodeficiency virus sequences by using in vitro enzymatic amplification and oligomer cleavage detection. J. Virol.61 (1987) 1690–1694.
Leser, U., Testung und Etablierung einer Methode für die Nukleinsäurehybridisierung bei menschlichen Herpesviren (Schwerpunkt: Cytomegalievirus) mit klinischen Materialien. Thesis, LMU-München (1985).
Melton, D.A., Krieg, P.A., Rebagliati, M.R., Maniatis, T., Zinn, K., and Green, M.R., Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucl. Acids Res.12 (1984) 7035–7056.
Motz, M., Fan, J., Seibl, R., Jilg, W., and Wolf, H., Expression of the Epstein-Barr virus 138-kDa early protein in Escherichia coli for the use as antigen in diagnostic tests. Gene42 (1986) 303–312.
Parichehre, D., Rosenberg, A.H., Dunn, J.J., and Studier, F.W., Cloning and expression of the gene for bacteriophage T7 RNA polymerase. Proc. natl Acad. Sci. USA81 (1984) 2035–2039.
Raab-Traub, N., and Flynn, K., The structure of the termini of the Epstein-Barr virus as a marker of clonal cellular proliferation. Cell47 (1986) 883–889.
Richter, W., Gu, S.Y., Seibl, R., and Wolf, H., A new method for examination of carcinomas of the nasopharynx, in: Nasopharyngeal Carcinoma: Current Concepts, pp. 25–32. Eds U. Prasad, D.V. Ablashi, P. Levine and G. Pearson. University Malaya Press, Kuala Lumpur 1983.
Rigby, P., Dieckmann, M., Rhodes, C. and Berg, P., Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J. molec. Biol.113 (1977) 237–251.
Seelig, R., Metzger, B., Renz, M., Metzger, P., and Seelig, H.P., Diagnostische Bedeutung des Nachweises von Hepatitis-B-Virus-DNS bei akuten und chronischen Hepatitiden. Dtsch. med. Wschr.110 (1985) 1968–1974.
Seibl, R., and Wolf, H., Mapping of Epstein-Barr virus proteins on the genome by translation of hybrid-selected RNA from induced P3HR1 cells and induced Raji cells. Virology141 (1985) 1–13.
Wolf, H., zur Hausen, H., and Becker, V., EB viral genomes in epithelial nasopharyngeal carcinoma cells. Nature New Biol244 (1973) 245–247.
Wolf, H., Haus, M., Leser, U., Modrow, S., Motz, M., Gu, S.Y., Falser, N., Bandtlow, I., Richter, W., and Pathmanathan, R., New developments in nucleic acid hybridization, in: Virus-associated Cancers in Africa, pp. 373–391. Eds A.O. Williams, G.T. O'Conor, G.B. de Thé and C.A. Johnson. IARC Scientific Publications No. 63, Lyon, France 1984.
Wolf, H., Leser, U., Haus, M., Gu, S.Y., and Pathmanathan, R., Sandwich nucleic acid hybridization: A method with a universally usable labeled probe for various specific tests. J. virol. Meth.13 (1986) 1–8.
Wolf, H., Modrow, S., Motz, M., Jameson, B., Hermann, G., and Förtsch, B., An integrated family of amino acid sequence analysis programs. CABIOS, submitted.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wolf, H. Molecular biological methods in the diagnosis of viral disease. Experientia 43, 1189–1192 (1987). https://doi.org/10.1007/BF01945521
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF01945521