Skip to main content
SpringerLink
Log in

Ready to use p-Benzoquinone activated supports for biochemical coupling, with special applications for laccase immobilization

  • Published:
Biotechnology Techniques

Summary

A new type of p-Benzoquinone activated support (agarose, polyvinyl alcohol, chitosan) which can be stored for more than one year, is described. Coupling yields of 10–95% were obtained for various compounds (propylamine, serum albumin, alpha-amylase). A retained activity of 150% for the immobilized laccase, was obtained. This peculiar result is probably due to the presence of hydroquinone groups on the support, which, as a substrate of laccase, can generate a simultancous affinity retention of the enzyme.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bradford, M.M. (1976), Anal. Biochem., 72, 248–254

    Google Scholar 

  • Brandt, J., Andersson, L.O., and Porath, J. (1975), Biochim. Biophys. Acta, 386, 196–202.

    Google Scholar 

  • Krämer, D.M. (1988), The development of Eupergit C, in Biotechnology Focus 1, pp. 353–367, Carl Hanser Verlag, München, Wien.

    Google Scholar 

  • Mateescu, M.A., Schell, M.D., Dimonie, M., Bentia, T., Dimitriou, C. and Maior, O. (1981) Rev. Roumaine Chim., 26, 117–124.

    Google Scholar 

  • Mateescu, M.A., Schell, M.D., Dimonie, M., Todireanu, S. and Maior, O. (1984) Polymer Bulletin, 11, 471–427

    Google Scholar 

  • O'Neil, P., Fielden, E.M., Morpurgo, L., Agostinelli, E. (1984), Biochem. J. 222, 71–76.

    Google Scholar 

  • Neugebauer, W.A., Neugebauer, E., Brzezinski, R. (1988), Carbohydr. Res, 1, 89,363–367.

    Google Scholar 

  • Porath, J. and Axën, R. (1976), Methods in Enzymology 44, 19–45.

    Google Scholar 

  • Reinhammar, B. (1970), Biochem. Biophys. Acta 205, 35–47.

    Google Scholar 

  • Street, H.V. and Close, J.R. (1956), Clinica Acta, 1, 256–268.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Département de Chimie, Université du Québec à Montréal, Succ. A, C.P. 8888, H3C 3P8, Montréal, QC, Canada

    Mircea-Alexandru Mateescu, Grazyna Weltrowska, Richard Saint-André & Marek Weltrowski

  2. Department of Biochemical Sciences, University of Rome, “La Sapienza” and CNR-Center of molecular Biology, 00185, Rome, Italy

    Enzo Agostinelli & Bruno Mondovi

Authors
  1. Mircea-Alexandru Mateescu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Grazyna Weltrowska
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Enzo Agostinelli
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Richard Saint-André
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Marek Weltrowski
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Bruno Mondovi
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mateescu, MA., Weltrowska, G., Agostinelli, E. et al. Ready to use p-Benzoquinone activated supports for biochemical coupling, with special applications for laccase immobilization. Biotechnol Tech 3, 415–420 (1989). https://doi.org/10.1007/BF01875011

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01875011

Keywords

Search

Navigation