Summary
In search for new techniques of renal preservation, kidneys were persufflatedin situ with gaseous O2 at a pressure of 90–100 mm Hg via their vascular system (orthograde aerobic ischemia). The results on post-ischemic function and blood flow, and on the changes in the renal tissue levels of the adenine nucleotides were compared to those obtained from kidneys ischemically stored without additional measures (anaerobic ischemia). The kidneys were stored for 4 hrs at 6°C. A contralateral nephrectomy was performed before the preservation period.
All dogs subjected to renal anaerobic ischemia survived. After orthograde aerobic ischemia, 3 out of 4 dogs died from uremia within 8 days, 1 animal survived. Although the aerobic ischemic preservation resulted in the maintenance of a metabolic status characteristic for aerobiosis, post-ischemic renal function recovered too slowly and insufficiently on the average. Renal blood flow was within normal ranges after anaerobic ischemia, it was reduced to about one third of this after orthograde aerobic ischemia.
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Dedicated to Prof. Dr. Dr. R. Zenker for his 70th birthday.
Supported by a grant from the Deutsche Forschungsgemeinschaft in the Sonderforschungsbereich 68.
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Isselhard, W., Denecke, H., Stelter, W. et al. Function and metabolism of canine kidneys after aerobic ischemia by orthograde persufflation with gaseous oxygen. Res. Exp. Med. 159, 288–297 (1973). https://doi.org/10.1007/BF01851603
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DOI: https://doi.org/10.1007/BF01851603