Abstract
Allele and genotype frequencies for 7 tetramerie short tandem repeat loci were determined in a Spanish population sample (N = 186–244) using PCR and subsequent analysis of the PCR products by denaturing polyacrylamide gel electrophoresis followed by silver staining. The loci were HUMFES/FPS, HUMVWA, HUMTHOI, HUMF13B, HUMCSFIPO, HUMF13A1 and HUMTPOX and all loci met Hardy-Weinberg expectations. In addition, little evidence was found for association of alleles among the 7 loci. Thus the allele frequency data can be used in identity testing to estimate the frequency of a multiple PCR-based DNA profile in the Spanish population.
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Edwards A, Civitello A, Hammond HA, Caskey CT (1991) DNA typing and genetic mapping with trimeric and tetrameric tandem repeats. Am J Hum Genet 49:746–756
Edwards A, Hammond HA, Jin L, Caskey CT, Chakraborty R (1992) Genetic variation at five trimeric and tetrameric repeat loci in four human population groups. Genomics 12:241–253
Saiki RK, Scharf S, Faloona T, Mullis KB, Horn GT, Erlich HA, Arnheim N (1985) Enzymatic amplification of β-globin genomic sequences and restriction analysis for diagnosis of sickle cell anemia. Science 230:1350–1354
Hagelberg E, Gray IC, Jeffreys AJ (1991) Identification of the skeletal remains of a murder victim by DNA analysis. Nature 352:427
Gill P, Kimpton CP, Sullivan KM (1992) A rapid polymerase chain reaction method for identifying fixed specimens. Electrophoresis 13:173–175
Jeffreys AJ, Allen MJ, Hagelberg E, Sonnberg A (1992) Identification of the skeletal remains of Joseph Mengele by DNA analysis. Forensic Sci Int 56:65–76
Hammond HA, Jin L, Zhong Y, Caskey CT, Chakraborty R (1994) Evaluation of 13 short tandem repeat loci for use in personal identification applications. Am J Hum Genet 55:175–189
Polymeropoulos MH, Rath DS, Xiao H, Merril CR (1991) Tetranucleotide repeat polymorphism at the human c-fes/fps proto-oncogene (FES). Nucleic Acids Res 19:4018
Kimpton CP, Walton A, Gill P (1992) A further tetranucleotide repeat polymorphism in the vWF gene. Hum Mol Genet 1:287
Nishimura DY, Murray JC (1992) A tetranucleotide repeat for the F13B locus. Nucleic Acids Res 20:1167
Polymeropoulos MH, Rath DS, Xiao H, Merril CR (1991) Tetranucleotide repeat polymorphism at the human coagulation factor XIII A subunit gene (F13A1). Nucleic Acids Res 19:4306
Anker R, Steinbrueck T, Dormis-Keller H (1992) Tetranucleotide repeat polymorphism at the human thyroid peroxidase (hTPO) locus. Hum Mol Genet 1:137
Urquhart A, Kimpton CP, Downes TJ, Gill P (1994) Variation in short tandem repeat sequences: a survey of twelve microsatellite loci for use as forensic identification markers. Int J Leg Med 107:13–20
Huang NE, Schumm J, Budowle B (1995) Chinese population data on three tetrameric short tandem repeat loci - HUMTHO1, TPOX, and CSF1PO - derived using multiplex PCR and manual typing. Forensic Sci Int 71:131–136
Budowle B, Chakraborty R, Giusti AM, Eisenberg AJ, Allen RC (1991) Analysis of the VNTR locus D1S80 by the PCR followed by high-resolution PAGE. Am J Hum Genet 48:137–144
DNA recommendations (1994) Report concerning further recommendations of the DNA commission of the ISFH regarding PCR-based polymorphisms in STR (short tandem repeat) systems. Int J Legal Med 107: 159–160
Chakraborty R, Smouse PE, Neel JV (1988) Population amalgamation and genetic variation: observations on artificially agglomerated tribal populations of Central and South America. Am J Hum Genet 43:709–725
Chakraborty R, Fornge M, Guegue R, Boerwinkle E (1991) Population genetics of hypervariable loci: analysis of PCRbased VNTR polymorphism within a population. In: Burke T, Dolf G, Jeffreys AJ, WolffR (eds) DNA fingerprinting: approaches and applications. Birkhauser, Berlin, pp 127–143
Nei M, Roychoudhury AK (1974) Sampling variances of heterozygosity and genetic distance. Genetics 76:379–390
Nei M (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89:583–590
Weir BS (1992) Independence of VNTR alleles defined by fixed bins. Genetics 130:873–887
Guo SW, Thompson EA (1992) Performing the exact test of Hardy-Weinberg proportion for multiple alleles. Biometrics 48:361–372.
Karlin S, Cameron EC, Williams PT (1981) Sibling and parentoffspring correlation estimation with variable family size. Proc Natl Acad Sci USA 78:2664–2668
Brown AHD, Feldman MW, Nevo E (1980) Multilocus structure of natural populations of Hordeum Spontaneum. Genetics 96:523–536
Chakraborty R (1984) Detection of nonrandom associations of alleles from the distribution of the number of heterozygous loci in a sample. Genetics 108:719–731
Chakraborty R., Zhong Y, Jin L, Budowle B (1994) Non-detectability of restriction fragments and independence of DNAfragment sizes within and between loci in RFLP typing of DNA. Am J Hum Genet 55:391–401
Puers C, Hammond HA, Jin L, Caskey CT, Schumm JW (1993) Identification of repeat sequence heterogeneity at the polymorphic short tandem repeat locus HUMTHO1 [AATG]n and reassignment of alleles in population analysis by using a locus-specific allelic ladder. Am J Hum Genet 53:953–958
Schlotterer C, Tautz D (1992) Slippage synthesis of simple sequence DNA. Nucleic Acids Res 20:211–215
Weir BS (1990) Multiple tests. In: Genetic data analysis. Sinauer Associate, Sunderland, Mass, pp 109–110
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Martin, P., Alonso, A., Budowle, B. et al. Spanish population data on 7 tetrameric short tandem repeat loci. Int J Leg Med 108, 145–149 (1995). https://doi.org/10.1007/BF01844826
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DOI: https://doi.org/10.1007/BF01844826