Summary
The hormone dependency of the MCF-7 breast cancer cell line, while extensively tested in liquid culture, has not been previously evaluated under conditions of anchorage-independent growth in serum-free media. Using the soft agar clonogenic assay, we demonstrate that physiologically relevant concentrations of estradiol (E2), progesterone (Pg), and prolactin (PRL) similarly stimulated MCF-7 cell colony formation in the absence of serum. Addition of an anti-insulin-like growth factor-I (IGF-I) antibody inhibited E2- and Pg-stimulated growth, while PRL action was not affected. Similar results were obtained with an anti-IGF-I receptor antibody, except that its inhibitory effect on Pg-induced colony formation was modest and not statistically significant. Administration of either an anti-transforming growth factor-α (TGF-α) antibody or an anti-epidermal growth factor (EGF) receptor antibody similarly inhibited E2-stimulated MCF-7 cell growth in soft agar, while neither antibody influenced Pg or PRL effects. Addition of TGF-β1, -β2, -β3 similarly suppressed MCF-7 cell colony formation in a dose dependent manner to a degree comparable to that observed with 4-OH-tamoxifen (4-OH-T). Furthermore, the growth inhibitory effect of 4-OH-T was completely reversed by an anti-TGF-β antibody. We conclude that IGFs and TGF-α are important mediators of E2-stimulated MCF-7 cell growth in soft agar. IGFs may also be playing a role in Pg action, while neither growth factor is involved in PRL-stimulated colony formation. Finally, TGF-β appears to be an important mediator of antiestrogen-induced inhibition of tumor growth.
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Abbreviations
- IGF:
-
insulin-like growth factor
- TGF:
-
transforming growth factor
- E2 :
-
estradiol-17β
- Pg:
-
progesterone
- PRL:
-
prolactin
- 4-OH-T:
-
4-hydroxy-tamoxifen
- IMEM:
-
improved minimal essential medium
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Manni, A., Wright, C. & Buck, H. Growth factor involvement in the multihormonal regulation of MCF-7 breast cancer cell growth in soft agar. Breast Cancer Res Tr 20, 43–52 (1991). https://doi.org/10.1007/BF01833356
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DOI: https://doi.org/10.1007/BF01833356