Abstract
Infection with HSV-1 is accompanied by the shut-off of cellular gene expression. The virion-associated function is encoded by the viral gene UL41. An HSV-1 mutant, vhs-1, which has a genomic deletion in the UL41 gene, is incapable of inducing the shut-off of cellular gene expression. The effect of HSV-1 infection on the shut-off of the cellular genes (or mRNA degradation) was studied specifically with the cellular genes for β-actin, fibronectin, glucose transporter-1, and the docking protein. The level of these specific mRNAs was measured in cells infected with several HSV-1 strains and was compared to that of vhs-1- and mock-infected cells. It was possible to demonstrate a marked reduction in the level of the specific mRNA from these cellular genes in cells infected with several HSV-1 strains but not with the vhs-1 mutant. The pathogenicity of the HSV-1 vhs-1 mutant to newborn mice was studied. It was found that the mutant is less pathogenic to newborn mice than its parental strain HSV-1 KOS.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Fenwick M.L. and Walker M.J., J Gen Virol41 37–51, 1978.
Sidiskis R.J. and Roizman B., Science153 76–78, 1966.
Nishioka Y. and Silverstern S., J Gen Virol27 619–627, 1978.
Fenwick M.L. and Everett R.D., J Gen Virol71 2961–2967, 1990.
Fenwick M.L. and McMenamin M.M., J Gen Virol65 1225–1228, 1984.
Mayman B.A. and Nishioka Y., J Virol53 1–6, 1985.
Scheck N. and Bachenheimer S.L., J Virol55 601–610, 1985.
Kwong A.D., Kruger J.A., and Frenkel N., J Virol62 912–921, 1988.
Strom T. and Frenkel N., J Virol6 2198–2207, 1978.
Everett R.D. and Fenwick M.L., J Gen Virol71 1387–1390, 1990.
Bastow K.F., Zhou B.S., and Chang Y.C., Virus Genes2 357–365, 1989.
Daksis J.I. and Chan V.L., J Virol61 143–150, 1987.
Enk C., Sprecher E., and Becker Y., Arch Virol121 141–151, 1991.
Dienes H.P., Knoblich A., and Falke D., Arch Virol86 223–237, 1975.
Ruoslathi E., Ann Rev Biochem57 375–413, 1988.
Filer J.S., Muckler M., McCall A.L., and Lodish H.F., J Clin Invest79 657–661, 1987.
Wickner W.T. and Lodish H.F., Science230 400–407, 1985.
Wessels H.P. and Spiess M., Cell55 61–70, 1988.
Von Heijne G., EMBO J.5 3021–3027, 1986.
Saier M.H., Werner P.K., and Müller M., Microbiol Rev53 333–366, 1989.
Rösen A., Ernst E., Koch H.G., Gelderblom H., Darai G., Hadar J., Tabor E., Ben Hur T., and Becker Y., Virus Res5 157–175, 1986.
Becker Y., Hadar J., Tabor E., Ben Hurt T., Raibstein I., Rösen A., and Dari G., Virology149 255–259, 1986.
Rösen A., Gelderblom H., and Darai G., Med Microbiol Immunol173 257–278, 1985.
Aldberg A., Linney E., and Ruoslahti E., J Biol Chem258 10193–10196, 1988.
Flier J.S., Mueckler M., McCall A.L., and Lodish H.F.J., Clin Invest79 657–661, 1987.
Muckler M., Carnso C., Baldwin S.A., Pancio M., Blench I., and Moris H.R., Science229 941, 1985.
Chirgwin J.M., Przybyla A.E., MacDonald R.S., and Rutter W.S., Biochemistry18 5294–5299, 1979.
Thomas P.S., Proc Natl Acad Sci USA71 5201–5205, 1980.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Becker, Y., Tavor, E., Asher, Y. et al. Effect of herpes simplex virus type-1 UL41 gene on the stability of mRNA from the cellular genes: β-actin, fibronectin, glucose transporter-1, and docking protein, and on virus intraperitoneal pathogenicity to newborn mice. Virus Genes 7, 133–143 (1993). https://doi.org/10.1007/BF01702393
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01702393