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Development ofgusA reporter gene constructs for cereal transformation: Availability of plant transformation vectors from the CAMBIA Molecular Genetic Resource Service

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Abstract

The use of reporter genes to characterise sequence elements that act to regulate gene expression in transgenic plants has been vital to the development of foreign gene expression strategies for use in cereal transformation. ThegusA locus ofEscherichia coli, which encodes the enzymeβ-glucuronidase (GUS), is by far the most popular reporter gene used in plant transformation. In this paper we extend the utility of the GUS reporter gene system in cereal transformation by describing and evaluating a number of novel constructs suitable for use in direct gene transfer experiments. These plasmids are all available from the Molecular Genetic Resource Service of the Center for the Application of Molecular Biology to International Agriculture.

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Author notes

  1. David McElroy

    Present address: USDA/ARS Plant Gene Expression Center, 800 Buchanan Street, 94710, Albany, CA, USA

  2. Douglas A. Chamberlain

    Present address: Centre for Molecular Biotechnology, Queensland University of Technology, 4060, Brisbane, QLD, Australia

Authors and Affiliations

  1. Division of Plant Industry, CSIRO, GPO Box 1600, 2601, Canberra, ACT, Australia

    David McElroy & Douglas A. Chamberlain

  2. Department of Biology, Ajou University, Suwon, Republic of Korea

    Eunpyo Moon

  3. Center for the Application of Molecular Biology to International Agriculture, CAMBIA, GPO Box 3200, 2601, Canberra, ACT, Australia

    Kate J. Wilson

Authors
  1. David McElroy
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  2. Douglas A. Chamberlain
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  3. Eunpyo Moon
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  4. Kate J. Wilson
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McElroy, D., Chamberlain, D.A., Moon, E. et al. Development ofgusA reporter gene constructs for cereal transformation: Availability of plant transformation vectors from the CAMBIA Molecular Genetic Resource Service. Mol Breeding 1, 27–37 (1995). https://doi.org/10.1007/BF01682087

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  • DOI: https://doi.org/10.1007/BF01682087

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