Summary
In this paper we have described the microbiology of the granuloma pouch model in rats. We studied the biochemical parameters of pouch exudates infected withEscherichia coli. Data revealed that the inflammatory response increased during the course of infection since lactate dehydrogenase levels as well as α2 and γ-globulin fractions were increased in comparison to uninfected controls. Infection of the pouches did not spread. We also monitored the growth characteristics of four differentE. coli strains.In vitro incubation of these strains in human and rat serum as well as in pouch exudate, and thein vivo growth rate in infected pouches revealed that the degree of serum sensitivity was clearly related to viabilityin vivo. Serum-resistant strains grew well in pouch exudate, whereas serum-sensitive strains were eliminated from the infected pouches. Since elimination of these strains was dependent on the challenge dose, we concluded that cellular and/or humoral host defense mechanisms became locally exhausted or inactivated. Thus, the granuloma pouch represents a local bacterial infection of a poorly defended, inflamed body cavity.
Zusammenfassung
Die Mikrobiologie des „Granuloma pouch“-Modells in Ratten wird beschrieben. Biochemische Untersuchungen von Pouch-Exsudaten, die mitEscherichia coli infiziert worden waren, ergaben, daß im Verlauf der Infektion die entzündliche Reaktion gesteigert wird, da die Laktat-Dehydrogenase-Spiegel und α2-sowie γ-Globulin-Fraktionen im Vergleich zu nicht infizierten Kontrollen erhöht waren. Die Infektion generalisierte sich nicht. Die Wachstumscharakteristika von vier unterschiedlichenE. coli-Stämmen wurden aufgezeichnet. Das Wachstumsverhalten der Stämmein vitro in menschlichem oder Rattenserum sowie Pouch-Exsudat wurdein vivo in infizierten Pouchen widergespiegelt. Das Ausmaß der Serumempfindlichkeit der Stämme entsprach eindeutig denIn-vivo-Wachstumsraten; Serum-resistente Stämme wuchsenin vivo sehr gut im Pouch-Exsudat, wogegen Serum-empfindliche Stämme von den infizierten Pouchen eliminiert wurden. Da die Elimination dieser Stämme von der Infektions-dosis abhängig war, wird angenommen, daß die Bestandteile der zellulären und/oder humoralen Körperabwehr lokal „verbraucht“ oder inaktiviert werden. Somit stellt die Granuloma-Pouch eine lokale bakterielle Infektion in einer abwehrgeschwächten entzündeten Körperhöhle dar.
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Dalhoff, A., Frank, G. & Luckhaus, G. The granuloma pouch: An in vivo model for pharmacokinetic and chemotherapeutic investigations. II. Microbiological characterization. Infection 11, 41–46 (1983). https://doi.org/10.1007/BF01651357
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DOI: https://doi.org/10.1007/BF01651357