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Chronic demyelination in mouse peripheral nerve produced by lysophosphatidyl choline and X-irradiation: ultrastructural observations

  • Published:
Journal of Neurocytology

Summary

The effects of X-irradiation on demyelination and remyelination were studied in the peripheral nerve of the mouse. Three days after injection of lysophosphatidyl choline into one sciatic nerve, a 20 Gy dose of X-rays was administered to the hind limb. At survival times ranging from 4 days to 6 months after injection, the nerves were examined by light and electron microscopy. Removal of myelin debris was retarded and remyelination delayed or prevented. The myelin sheaths which did form were thin and the configuration of Schmidt-Lanterman incisures and nodes of Ranvier was abnormal. Some of the chronically demyelinated fibres formed focal node-like complexes: patches of finely granular material coated the inner aspect of the axolemma, the external surface was covered by slender processes of Schwann cell cytoplasm, and an electron-dense lamina was present in the enlarged periaxonal space. Elsewhere demyelinated axons and their ensheathing Schwann cells were separated by gap junctions or transverse bands. The present findings indicate that the morphological differentiation of structures thought to be characteristic of nodes of Ranvier can take place in the absence of remyelination.

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References

  • Aguayo, A., Epps, J., Charron, L. &Bray, G. (1976) Multipotentiality of Schwann cells in cross-anastomosed and grafted myelinated and unmyelinated nerves: quantitative microscopy and radioautography.Brain Research 104, 1–20.

    Google Scholar 

  • Aguayo, A. J., Romine, J. S. &Bray, G. M. (1975) Experimental necrosis and arrest of proliferation of Schwann cells by cytosine arabinoside.Journal of Neurocytology 4, 663–74.

    Google Scholar 

  • Asbury, A. K. (1975) The biology of Schwann cells. InPeripheral Neuropathy edited byDyck, P. J., Thomas, P. K. &Lambert, E. H.), pp. 201–12. Philadelphia: W. B. Saunders.

    Google Scholar 

  • Blakemore, W. F. (1978) Observations on remyelination in the rabbit spinal cord following demyelination induced by lysolecithin.Neuropathology and Applied Neurobiology 4, 47–59.

    Google Scholar 

  • Cavanagh, J. B. (1968a) Effects of X-irradiation on the proliferation of cells in peripheral nerve during Wallerian degeneration in the rat.British Journal of Radiology 41, 275–81.

    Google Scholar 

  • Cavanagh, J. B. (1968b) Prior X-irradiation and the cellular response to nerve crush: duration of effect.Experimental Neurology 22, 253–8.

    Google Scholar 

  • Edds, M. V. (1955) Collateral regeneration in partially reinnervated muscles of the rat.Journal of Experimental Zoology 129, 225–47.

    Google Scholar 

  • Gregson, N. A. &Hall, S. M. (1973) A quantitative analysis of the effects of the intraneural injection of lysophosphatidyl choline.Journal of Cell Science 13, 257–77.

    Google Scholar 

  • Hall, S. M. (1972) The effect of injections of lysophosphatidyl choline into white matter of the adult mouse spinal cord.Journal of Cell Science 10, 535–46.

    Google Scholar 

  • Hall, S. M. (1973) Some aspects of remyelination after demyelination produced by the intraneural injection of lysophosphatidyl choline.Journal of Cell Science 13, 461–77.

    Google Scholar 

  • Hall, S. M. (1984) The effects of multiple sequential episodes of demyelination in the sciatic nerve of the mouse.Neuropathology and Applied Neurobiology 10, 461–78.

    Google Scholar 

  • Hall, S. M. &Gregson, N. A. (1971) The in vivo and ultrastructural effects of injection of lysophosphatidyl choline into myelinated peripheral nerve fibres of the adult mouse.Journal of Cell Science 9, 769–89.

    Google Scholar 

  • Hall, S. M. &Gregson, N. A. (1975) The effects of mitomycin C on the process of remyelination in the mammalian peripheral nervous system.Neuropathology and Applied Neurobiology 1, 149–70.

    Google Scholar 

  • Hall, S. M. &Gregson, N. A. (1977) The effects of mitomycin C on the process of regeneration in the mammalian peripheral nervous system.Neuropathology and Applied Neurobiology 3, 65–78.

    Google Scholar 

  • Hirano, A. (1984) Nodes of Ranvier in pathological conditions. InThe Node of Ranvier (edited byZagoren, J. C. &Fedoroff, S.), pp. 213–43. Orlando: Academic Press.

    Google Scholar 

  • Jacobs, J. M. &Cavanagh, J. B. (1969) Species difference in internode formation following two types of peripheral nerve injury.Journal of Anatomy 105, 295–306.

    Google Scholar 

  • King, R. H. M., Pollard, J. D. &Thomas, P. K. (1975) Aberrant remyelination in chronic relapsing experimental allergic neuritis.Neuropathology and Applied Neurobiology 1, 367–78.

    Google Scholar 

  • Landon, D. N. &Langley, O. K. (1971) The local chemical environment of nodes of Ranvier: a study of cation binding.Journal of Anatomy 108, 419–32.

    Google Scholar 

  • Langley, O. K. (1969) Ion exchange at the node of Ranvier.Histochemical Journal 1, 295–309.

    Google Scholar 

  • Langley, O. K. &Landon, D. N. (1967) A light and electron histochemical approach to the nodes of Ranvier and myelin of peripheral nerve fibres.Journal of Histochemistry and Cytochemistry 15, 722–31.

    Google Scholar 

  • Love, S. (1983a) An experimental study of peripheral nerve regeneration after x-irradiation.Brain 106, 39–54.

    Google Scholar 

  • Love, S. (1983b) Effects of radiation-induced hypomyelinating neuropathy on skeletal muscle in the mouse: morphological and physiological studies. PhD Thesis, University of London.

  • Love, S. &Gomez, S. (1984) Effects of experimental radiation-induced hypomyelinating neuropathy on motor end-plates and neuromuscular transmission.Journal of the Neurological Sciences 65, 93–109.

    Google Scholar 

  • Mitchell, J. &Caren, C. A. (1982) Degeneration of non-myelinated axons in the rabbit sciatic nerve following lysolecithin injection.Acta neuropathologica (Berlin) 56, 187–93.

    Google Scholar 

  • Moss, T. H. &Lewkowicz, S. J. (1985) Effect of ionising radiation on the axon reaction of mouse anterior horn motor neurons. A histological and immunohistochemical study using a monoclonal antibody to neurofilament protein.Journal of the Neurological Sciences 67, 1–14.

    Google Scholar 

  • Puck, T. T. (1959) Quantitative studies on mammalian cells in vitro.Reviews of Modern Physics 31, 433–48.

    Google Scholar 

  • Puck, T. T. &Marcus, P. I. (1956) Action of x-rays on mammalian cells.Journal of Experimental Medicine 103, 653–66.

    Google Scholar 

  • Rosenbluth, J. (1984) Membrane specializations at nodes of Ranvier and paranodal and juxtaparanodal regions of myelinated central and peripheral nerve fibers. InThe Node of Ranvier (edited byZagoren, J. C. &Fedoroff, S.), pp. 31–67. Orlando: Academic Press.

    Google Scholar 

  • Rosenbluth, J. (1985) Normal and abnormal axolemmal structure in freeze-fractured myelinated fibers. InThe Pathology of the Myelinated Axon (edited byAdachi, M., Hirano, A. &Aronson, S. M.), pp. 1–29. New York: Igaku-Shoin.

    Google Scholar 

  • Simpson, S. A. &Young, J. Z. (1945) Regeneration of fibre diameter after cross-unions of visceral and somatic nerves.Journal of Anatomy 79, 48–65.

    Google Scholar 

  • Smith, K. J., Bostock, H. &Hall, S. M. (1982) Saltatory conduction precedes remyelination in axons demyelinated with lysophosphatidyl choline.Journal of the Neurological Sciences 54, 13–31.

    Google Scholar 

  • Smith, K. J. &Hall, S. M. (1980) Nerve conduction during peripheral demyelination and remyelination.Journal of the Neurological Sciences 48, 201–19.

    Google Scholar 

  • Streline, G. S. &Evsukov, V. I. (1965) Dépression et réparation du pouvoir régénerateur du nerf périphérique après application de rayons X.Biologie Medicale 54, 466–76.

    Google Scholar 

  • Suzuki, K. &Zagoren, J. C. (1977) Quaking mouse: an ultrastructural study of the peripheral nerves.Journal of Neurocytology 6, 71–84.

    Google Scholar 

  • Van Deenen, L. L. M. &Dehaas, G. H. (1966) Phosphoglycerides and phospholipases.Annual Review of Biochemistry 35, 157–94.

    Google Scholar 

  • Waxman, S. G., Kocsis, J. D. &Nitta, K. C. (1979) Lysophosphatidyl choline-induced focal demyelination in the rabbit corpus callosum.Journal of the Neurological Sciences 44, 45–53.

    Google Scholar 

  • Webster, G. R. (1961) Some aspects of lipid metabolism in nervous tissue.International Review of Neurobiology 3, 293–317.

    Google Scholar 

  • Weinberg, H. J. &Spencer, P. S. (1975) Studies on the control of myelinogenesis. I. Myelination of regenerating axons after entry into a foreign unmyelinated nerve.Journal of Neurocytology 4, 395–418.

    Google Scholar 

  • Weinberg, H. J. &Spencer, P. S. (1976) Studies on the control of myelinogenesis. II. Evidence for neuronal regulation of myelin formation.Brain Research 117, 363–78.

    Google Scholar 

  • Wiley-Livingstone, C. A. &Ellisman, M. H. (1982) Return of axonal and glial membrane specializations during remyelination after tellurium-induced demyelination.Journal of Neurocytology 11, 65–80.

    Google Scholar 

  • Williams, P. L. &Hall, S. M. (1971) Chronic Wallerian degeneration: an in vivo and ultrastructural study.Journal of Anatomy 109, 487–503.

    Google Scholar 

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Authors and Affiliations

  1. Department of Neuropathology, Institute of Neurology, Queen Square, WC1N 3BG, London, UK

    S. Love & J. M. Jacobs

  2. MRC Cyclotron Unit, Hammersmith Hospital, W12 0HS, London, UK

    R. Myers

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  1. S. Love
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  2. J. M. Jacobs
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  3. R. Myers
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Love, S., Jacobs, J.M. & Myers, R. Chronic demyelination in mouse peripheral nerve produced by lysophosphatidyl choline and X-irradiation: ultrastructural observations. J Neurocytol 15, 155–167 (1986). https://doi.org/10.1007/BF01611652

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  • DOI: https://doi.org/10.1007/BF01611652

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