Abstract
Although salivary calcification is relatively common, little is known about the localization and content of the calcium of normal and diseased human salivary glands. We investigated this in chronic submandibular sialadenitis with a variable mixture of relatively normal and extremely atrophic parenchyma and in normal submandibular, parotid and palatal glands. Calcium was localized histochemically in mucous acinar cells of submandibular and palatal glands at moderate to high levels, in serous acinar cells of submandibular and parotid glands at low to moderate or occasionally high levels, in mucous ductal cells at moderate to high levels, and in extremely atrophic parenchyma at low levels or not at all. Calcium was determined biochemically at relatively high levels in the different glands in the order palatal, submandibular, sialadenitis and parotid. However, the differences were small. The results indicate that most salivary calcium is associated with secretory granules; this is the likely source of the calcium involved in salivary calcification
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Cohen L, Holliday M (1982) Statistics for social scientists. An introductory text with computer programs in BASIC. Harper and Row, London, pp 267–272
Epivatianos A, Harrison JD (1989) The presence of microcalculi in normal human submandibular and parotid salivary glands. Arch Oral Biol 34:261–265
Epivatianos A, Harrison JD, Dimitriou T (1987) Ultrastructural and histochemical observations on microcalculi in chronic submandibular sialadenitis. J Oral Pathol 16:514–517
Harrison JD (1974) Minor salivary glands of man: enzyme and mucosubstance histochemical studies. Histochem J 6:633–647
Harrison JD, Epivatianos A (1990) Preliminary observations on microliths and liths in chronic submandibular sialadenitis. J Dent Res 69:964
Harrison JD, Epivatianos A (1992) Production of microliths and sialadenitis in rats by a short combined course of isoprenaline and calcium gluconate. Oral Surg 73:585–590
Harrison JD, Auger DW, Paterson KL, Rowley PSA (1987) Mucin histochemistry of submandibular and parotid salivary glands of man: light and electron microscopy. Histochem J 19:555–564
Izutsu K, Wilkinson L, Oda D, Kayton R, Chen SW, Cantino M, Johnson D (1991) Comparison of elemental concentrations in the acinar cells of the human labial salivary gland. Arch Oral Biol 36:727–735
Kashiwa HK, Atkinson WB (1963) The applicability of a new Schiff base, glyoxal bis(2-hydroxy-anil), for the cytochemical localization of ionic calcium. J Histochem Cytochem 11:258–264
Kashiwa HK, House CM (1964) The glyoxalbis(2-hydroxyanil) method modified for localizing insoluble calcium salts. Stain Technol 39:359–367
Kousvelari EE, Baratz RS, Burke B, Oppenheim FG (1980) Immunochemical identification and determination of proline-rich proteins in salivary secretions, enamel pellicle, and glandular tissue specimens. J Dent Res 59:1430–1438
Kraintz L (1966) A comparison of the calcium concentration of submaxillary salivary glands. Nature 209:215–216
Mangos JA (1979) Morphological and functional characterization of isolated human parotid acinar cells. J Dent Res 58:2028–2035
Mangos JA, Donnelly WH (1981) Isolated parotid acinar cells from patients with cystic fibrosis. Morphology and composition. J Dent Res 60:19–25
Menden EE, Brockman D, Choudhury H, Petering HG (1977) Dry ashing of animal tissues for atomic absorption spectrometric determination of zinc, copper, cadmium, lead, iron, manganese, magnesium and calcium. Anal Chem 49:1644–1645
Roomans GM, Müller RM, Sagström S, Sagulin G-B, Scarlett SM, Wroblewski J, Albertsson M (1989) X-ray microanalysis of mammalian salivary glands. Scanning Microsc 3:225–241
Schäfer H (1979) Zellcalcium und Zellfunktion. Cytochemische, ultrastrukturelle und röntgenmikroanalytische Untersuchungen. Veroff Pathol 109
Scott J (1979) The proportional volume of mucous acinar cells in normal human submandibular salivary glands. Arch Oral Biol 24:479–481
Tandler B, Riva A (1986) Salivary glands. In: Mjör IA, Fejerskov O (eds) Human oral embryology and histology. Munksgaard, Copenhagen, pp 243–284
Triantafyllou A (1991) Microlithiasis of the major salivary glands of cat: a morphological, histochemical and biochemical study. Ph D Thesis, University of London
Triantafyllou A, Harrison JD, Garrett JR, Kidd A (1992) Increase of microliths in inactive salivary glands of cat. Arch Oral Biol 37:663–666
Verdugo P, Deyrup-Olsen I, Aitken M, Villalon M, Johnson D (1987) Molecular mechanism of mucin secretion: I. The role of intragranular charge shielding. J Dent Res 66:506–508
Westhofen M, Schäfer H, Seifert G (1984) Calcium redistribution, calcification and stone formation in the parotid gland during experimental stimulation and hypercalcaemia. Cytochemical and X-ray microanalytical investigations. Virchows Arch [A] 402:425–438
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Harrison, J.D., Triantafyllou, A., Baldwin, D. et al. Histochemical and biochemical determination of calcium in salivary glands with particular reference to chronic submandibular sialadenitis. Vichows Archiv A Pathol Anat 423, 29–32 (1993). https://doi.org/10.1007/BF01606428
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DOI: https://doi.org/10.1007/BF01606428