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Identification ofMycobacterium shimoidei in a tuberculosis-like cavity by 16S ribosomal DNA direct sequencing

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Abstract

A new, well-documented case ofMycobacterium shimoidei infection is reported. This bacterium was isolated from a patient with preexisting pulmonary lesions who developed a tuberculosis-like cavity in the lung. Isolation of fewer than ten strains, not epidemiologically related, has been reported worldwide; all originated from human pulmonary samples. Identification of the strain described here was first accomplished by 16S rDNA direct sequencing after in vitro amplification. This method allows rapid identification of unusual bacteria. The identification was confirmed by specific phenotypical tests and by chromatographic methods.

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References

  1. Tsukamura M, Shimoide H, Schaefer WB: A possible new pathogen of group III mycobacteria. Journal of General Microbiology 1975, 88: 377–380.

    Google Scholar 

  2. Tacquet A, Tison F: Nouvelle technique d'isolement des mycobactéries par le lauryl-sulfate de sodium. Annales de l'Institut Pasteur 1961, 100: 676–680.

    Google Scholar 

  3. Roberts GD, Koneman EW, Kim YK: Mycobacterium. In: Balows A, Hausler WJ, Herrmann KL, Isenberg HD, Shadomy HJ (ed): Manual of clinical microbiology (5th ed). American Society for Microbiology, Washington, DC, 1991, p. 304–339.

    Google Scholar 

  4. Rastogi N: Mycobacteria as intracellular pathogens: current notion of pathogenicity, virulence and drug resistance and their relation to effective therapy. In: Raoult D (ed): Antimicrobial agents and intracellular pathogens. CRC Press, Boca Raton, FL, 1993, p. 245–300.

    Google Scholar 

  5. De Lamballerie X, Zandotti C, Vignoli C, Bollet C, De Micco P: A one-step microbial DNA extraction method using “Chelex 100” suitable for gene amplification. Research in Microbiology 1992, 143: 785–790.

    Google Scholar 

  6. Brow MA: Sequencing with Taq DNA polymerase. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (ed): PCR protocols: a guide to methods and applications. Academic, San Diego, CA, 1990, p. 189–196.

    Google Scholar 

  7. Böddinghaus B, Rogall T, Flohr T, Blöcker H, Böttger EC: Detection and identification of mycobacteria by amplification of rRNA. Journal of Clinical Microbiology 1990, 28: 1751–1759.

    Google Scholar 

  8. Higgins DG, Sharp PM: Clustal: a package for performing multiple sequence alignment on a microcomputer. Gene 1988, 73:237–244.

    Google Scholar 

  9. De Briel D, Couderc F, Riegel P, Jehl F, Minck R: Highperformance liquid chromatography of corynomycolic acids as a tool in identification ofCorynebacterium species and related organisms. Journal of Clinical Microbiology 1992, 30: 1407–1417.

    Google Scholar 

  10. Thibert L, Lapierre S: Routine application of high-performance liquid chromatography for identification of mycobacteria. Journal of Clinical Microbiology 1993, 31: 1759–1763.

    Google Scholar 

  11. Lévy-Frébault V, Goh KS, David HL: Mycolic acid analysis for clinical identification ofMycobacterium avium and related mycobacteria. Journal of Clinical Microbiology 1986, 24: 835–839.

    Google Scholar 

  12. Vincent Lévy-Frébault V, Portaels F: Proposed minimal standards for the genusMycobacterium and for description of new slowly growingMycobacterium species. International Journal of Systematic Bacteriology 1992, 42:315–323.

    Google Scholar 

  13. Rogall T, Flohr T, Böttger EC: Differentiation ofMycobacterium species by direct sequencing of amplified DNA. Journal of General Microbiology 1990, 136: 1915–1920.

    Google Scholar 

  14. Kirschner P, Springer B, Vogel U, Meier A, Wrede A, Kiekenbeck M, Bange FC, Böttger EC: Genotypic identification ofMycobacteria by nucleic acid sequence determination: report of a 2-year experience in a clinical laboratory. Journal of Clinical Microbiology 1993, 31: 2882–2889.

    Google Scholar 

  15. Rüsh-Gerdes S, Wandelt-Freerksen E, Schröder KH: Vorkommen vonMycobacterium shimoidei in der Bundesrepublik Deutschland. Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene Series A 1985, 259: 146–150.

    Google Scholar 

  16. Tortoli E, Simonetti MT: Isolation ofMycobacterium shimoidei from a patient with cavitary pulmonary disease. Journal of Clinical Microbiology 1991, 29: 1754–1756.

    Google Scholar 

  17. Wayne LG, Sramek HA: Agents of newly recognised or infrequently encountered mycobacterial diseases. Clinical Microbiology Reviews 1992, 5: 1–25.

    Google Scholar 

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Heller, R., Jaulhac, B., Charles, P. et al. Identification ofMycobacterium shimoidei in a tuberculosis-like cavity by 16S ribosomal DNA direct sequencing. Eur. J. Clin. Microbiol. Infect. Dis. 15, 172–175 (1996). https://doi.org/10.1007/BF01591494

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