Abstract
A nicotinamide adenine dinucleotide (NAD)-dependent coniferyl alcohol dehydrogenase was enriched 1,200-fold from crude extracts ofRhodococcus erythropolis. The purification procedure involved ion exchange chromotography, gel filtration on Biogel A 1,5 and Sephadex G-200, and hydroxyapatite treatment. The enzyme had a molecular weight of approximately 200,000 and displayed maximal activity at pH 9.0. The apparentK m values for NAD and coniferyl alcohol were, respectively, 0.22 and 0.645 mM. Nicotinamide adenine dinucleotide phosphate (NADP) could only partially replace NAD. The enzyme was active with vanillyl alcohol and aromatic alcohols bearing the α,β-unsaturated side chain of coniferyl alcohols. These aromatic alcohols included the dilignols dehydrodiconiferyl alcohol and guaiacylglycerol-β-coniferyl ether.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature Cited
Bensadoun, A., Weinstein, D. 1976. Assay of proteins in the presence of interfering materials. Analytical Biochemistry70:241–250.
Eggeling, L., Sahm, H. 1980. Degradation of coniferyl alcohol and other lignin-related aromatic compounds byNocardia sp. DSM 1069. Archives of Microbiology126:141–148.
Farmer, V. C., Henderson, M. E. K., Russell, J. D. 1960. Aromatic-alcohol-oxidase activity in the growth medium ofPolysticus versicolor. Biochemical Journal74:257–262.
Gornall, A. G., Bardawill, C. J., David, M. M. 1949. Determination of serum proteins by means of the biuret reaction. Journal of Biological Chemistry177:751–766.
Grell, E. H., Jacobsen, K. B., Murphy, J. B. 1965. Alcohol dehydrogenases inDrosophila melanogaster: Isozymes and genetic variants. Science149:80–82.
Iwahara, S., Nishihira, T., Jomori, T., Kuwahara, M., Higuchi, T. 1980. Enzymic oxidation of α,β-unsaturated alcohols in the side chains of lignin-related aromatic compounds. Journal of Fermentation Technology58:183–188.
Jacobson, L. 1951. Maintenance of iron supply in nutrient solutions by a single addition offerric potassium ethylenediamine tetra-acetate. Plant Physiology25:411–413.
Katagiri, M., Takemori, S., Nakazawa, K., Suzuki, H., Akagi, K. 1967. Benzylalcohol dehydrogenase, a new alcohol dehydrogenase fromPseudomonas sp., Biochemica et Biophysica Acta139:173–176.
Katayama, T., Nakatsubo, F., Higuchi, T. 1980. Initial reactions in the fungal degradation of guaiacylglycerol-β-coniferyl ether, a lignin substructure model. Archives of Microbiology126:127–132.
Keat, M. J., Hopper, D. J. 1978. The aromatic alcohol dehydrogenases inPseudomonas putida N.C.I.B. 9869 grown on 3,5-xylenol andp-cresol. Biochemical Journal175:659–667.
Maurer, H. R. 1971. Disc electrophoresis, 2nd ed. Berlin, New York: Walter de Gruyter.
Ohta, M., Higuchi, T., Iwahara, S. 1979. Microbial degradation of dehydrodiconiferyl alcohol, a lignin substructure model. Archives of Microbiology121:23–28.
Russell, J. D., Henderson, M. E. K., Farmer, V. C. 1961. Metabolism of lignin model compounds byPolystictus versicolor. Biochimica et Biophysica Acta52:565–570.
Trojanowki, J., Haider, K., Sundman, V. 1977. Decomposition of14C-labelled lignin and phenols by aNocardia sp. Archives of Microbiology114:149–153.
Wichmann, R., Wandrey, C. 1980. On-line determination of reaction rate versus substrate concentration for an enzymatically catalyzed reaction by means of a microcomputer system, pp. 259–261. in: Weetall, H. H., Roger, G. P. (eds.), Enzyme engineering, vol. 5. New York, London: Plenum Press.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Jaeger, E., Eggeling, L. & Sahm, H. Partial purification and characterization of a coniferyl alcohol dehydrogenase fromRhodococcus erythropolis . Current Microbiology 6, 333–336 (1981). https://doi.org/10.1007/BF01567007
Issue Date:
DOI: https://doi.org/10.1007/BF01567007