Abstract
Twentyfour meniscal allotransplantations were conducted in 12 adult mongrel dogs. The medial meniscus was replaced using a deep-frozen meniscal allograft. The junction between the meniscus and capsule was treated in one of the three ways. In the control group, the meniscus was sutured only to the adjacent capsular tissue (group C). In the second group, fibrin glue was injected at the junction (group F), and in the third group, fibrin glue and endothelial cell growth factor (ECGF) were injected at the juncture between the transplanted meniscus and the adjacent capsule before the meniscus was sutured (group FE). Histological observation was performed to investigate the effect of fibrin glue and ECGF on the healing process of transplants at various intervals of 1, 4, 8 and 12 weeks. No immunological response was noted in any of the knees. The healing of the transplanted meniscus was first observed at the peripheral attachment. Also, the pannus-like tissue extended from the synovium to the surface of the meniscus. The healing rate in each group at 1 week and 12 weeks was 22% and 77% in group C, 52% and 80% in group F, and 64% and 80% in group FE, respectively. At 4 and 8 weeks, early cellular repopulation was found in group FE and the area which contained new cells was larger than the acellular central core at 8 weeks. However, there was no difference among the three groups at 12 weeks. The early stage of regeneration of the transplanted meniscus seemed to be accelerated by the use of fibrin glue and ECGF; however, they had little influence on the long-term results. Few cells were found in the central portion of the transplanted meniscus even at 12 weeks.
Similar content being viewed by others
References
Arnoczky SP, Warren RF, McDevitt CA (1990) Meniscal replacement using a cryopreserved allograft: an experimental study in the dog Clin Orthop 252: 122–128
Bylski-Austraw DI, Meade T, Malumed J, Noyes FR, Arnockzky SP, Schafer JA (1992) Irradiated meniscal allografts: mechanical and histological studies in the goat. Orthop Trans 17: 175
De Boer HH, Koudstaal J (1991) The fate of meniscus cartilage after transplantation of cryopreserved nontissue-antigenmatched allograft. Clin Orthop 266: 146–151
Garret JC, Stevensen RN (1991) Meniscal transplantation in the human knee: a preliminary report. Arthroscopy 7: 57–62
Gospodarowicz D (1974) Localization of a fibroblast growth factor and its effect alone and with hydrocortisone on 3T3 cell growth. Nature 249: 123–127
Gospodarowicz D, Greenburg G, Bialecki H, Zetter BR (1978) Factors involved in the modulation of cell proliferation in vivo and in vitro: the role of fibroblast and epidermal growth factors in the proliferative response of mammalian cells. In Vitro 14: 85–118
Hashimoto J, Kurosaka M, Yoshiya S, Hirohata K (1992) Meniscal repair using fibrin glue and endothelial cell growth factor (ECGF): an experimental study in dogs. Am J Sports Med 20: 537–541
Jackson DW, McDevitt CA, Atwell EA, Arnoczky SP (1990) Meniscal transplantation using fresh and cryopreserved allografts: an experimental study in goats. Orthop Trans 15: 221
Keating EM, Malinin TI, Belchic G (1988) Meniscal transplantation in goats: an experimental study. Orthop Trans 13: 147
McKibbin B, Ralis ZA (1978) The site dependence of the articular cartilage transplant reaction. J Bone Joint Surg [Br] 60: 561–566
Milachowski KA, Weismeier K, Wirth CJ (1989) Homologus meniscus transplantation: experimental and clinical results. Int Orthop 13: 1–11
Milton J, Flandry F, Terry G, Hunt J, Boosinger T, Brochbank K, McCaa C (1990) Transplantation of viable, cryopreserved menisci. Orthop Trans 15: 220
Rodan SB, Wesolowski G, Thomas K, et al (1987) Growth stimulation of calvaria osteoblastic cells by acidic fibroblast growth factor. Endocrinology 121: 1917–1923
Schlag G, Redl H, Turner M, Dinges HP (1986) The importance of fibrin in wound repair. In: Schlag G, Redl H (eds) Fibrin sealant in operative medicine. Springer, Berlin Heidelberg New York pp 3–12
Tachibana Y (1990) Meniscus transplantation using a cryopreserved allograft: an experimental study in the rabbit. (In Japanese) J Jpn Orthop Assoc 64: 583–592
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nabeshima, Y., Kurosaka, M., Yoshiya, S. et al. Effect of fibrin glue and endothelial cell growth factor on the early healing response of the transplanted allogenic meniscus: a pilot study. Knee Surg, Sports traumatol, Arthroscopy 3, 34–38 (1995). https://doi.org/10.1007/BF01553523
Issue Date:
DOI: https://doi.org/10.1007/BF01553523