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Gene expression in implanted rat hepatocytes following retroviral-mediated gene transfer

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Somatic Cell and Molecular Genetics

Abstract

An hepatocyte transplantation-gene transfer protocol has been developed whereby liver cells containing an expressing NeoR gene can be successfully implanted in vivo. Adult primary cultures of rat hepatocytes, after infection with the retroviral vector N2, were grown on a floating solid support (coated with purified collagen IV) in a serum-free hormonally defined medium designed for hepatocytes that also contained G418. Under these conditions, normal adult hepatocytes expressing the NeoR gene could be grown to high density. The solid supports holding the gene-engineered hepatocytes were then implanted into adult rats into subcutaneous and intraperitoneal sites. After one to two weeks, the supports were removed and shown to still contain the gene-engineered hepatocytes expressing the NeoR gene. These results suggest that cells from solid organs, such as the liver, are potential targets for gene transfer and expression studies in vivo.

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Author notes

  1. John A. Thompson

    Present address: Genetic Therapy Inc., 19 Firstfield Road, 20878, Gaithersburg, Maryland

Authors and Affiliations

  1. Laboratory of Molecular Hematology, National Heart, Lung, and Blood Institute, National Institutes of Health, 20892, Bethesda, Maryland

    Kathryn D. Anderson, John A. Thompson, Judith M. DiPietro & W. French Anderson

  2. Department of Surgery, Children's Hospital National Medical Center, 20010, Washington, D.C.

    Kathryn D. Anderson

  3. Departments of Molecular Pharmacology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, 10461, Bronx, New York

    Kate T. Montgomery & Lola M. Reid

  4. Microbiology and Immunology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, 10461, Bronx, New York

    Lola M. Reid

Authors
  1. Kathryn D. Anderson
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  2. John A. Thompson
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  3. Judith M. DiPietro
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  4. Kate T. Montgomery
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  5. Lola M. Reid
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  6. W. French Anderson
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Anderson, K.D., Thompson, J.A., DiPietro, J.M. et al. Gene expression in implanted rat hepatocytes following retroviral-mediated gene transfer. Somat Cell Mol Genet 15, 215–227 (1989). https://doi.org/10.1007/BF01534872

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  • DOI: https://doi.org/10.1007/BF01534872

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