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Integration of a dominant selectable marker into human chromosomes and transfer of marked chromosomes to mouse cells by microcell fusion

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Somatic Cell and Molecular Genetics

Abstract

A method for the production of stable mouse-human cell hybrids containing a single human chromosome is described. As a first step in this method, a cloned selectable marker, the E. coli xanthine-guanine phosphoribosyltransferase (Ecogpt) gene, was transferred to human cells to generate cell lines each carrying Ecogpt integrated into a different site. Human chromosomes marked with Ecogpt were transferred further into mouse cells by microcell fusion. Monochromosomal hybrids, in which the human chromosome is maintained by selection, have been produced for chromosomes 2, 5, 16, and a rearranged chromosome involving a translocation between chromosomes 1 and 2. In addition to these monochromosomal hybrids, we have also obtained monochromosomal hybrids for human chromosomes 6, 12, and 17 by selection for the loss of marked chromosome from the microcell hybrids each containing two human chromosomes. Although the human chromosome present in these hybrids cannot be maintained by selection, 80–90% of cells retained the transferred chromosome on continuous growth for 15 days. Monochromosomal hybrids would provide biological materials to construct genetic maps of human chromosomes. In addition, chromosomes marked with dominant selectable markers can be transferred further to any cell line of interest in inter- or intra-species combination.

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Authors and Affiliations

  1. Department of Microbiology, New Jersey Medical School, University of Medicine and Dentistry of N.J., 100 Bergen Street, 07103, Newark, New Jersey

    Raghbir S. Athwal, Maryalice Smarsh & Surinder S. Deo

  2. Department of Pediatrics, New Jersey Medical School, University of Medicine and Dentistry of N.J., 100 Bergen Street, 07103, Newark, New Jersey

    Bernard M. Searle

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  1. Raghbir S. Athwal
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  2. Maryalice Smarsh
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  3. Bernard M. Searle
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  4. Surinder S. Deo
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Athwal, R.S., Smarsh, M., Searle, B.M. et al. Integration of a dominant selectable marker into human chromosomes and transfer of marked chromosomes to mouse cells by microcell fusion. Somat Cell Mol Genet 11, 177–187 (1985). https://doi.org/10.1007/BF01534706

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  • DOI: https://doi.org/10.1007/BF01534706

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