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cDNA cloning and assignment to chromosome 21 ofIFI-78K gene, the human equivalent of murineMx gene

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Somatic Cell and Molecular Genetics

Abstract

Recently we have purified to homogeneity and characterized an interferon-induced human protein (p78 protein) which is the equivalent of the interferon-induced murine Mx protein responsible for a specific antiviral state against influenza virus infection. A cDNA library was constructed using mRNAs from interferon-induced human diploid fibroblasts. cDNA clones coding for the human p78 protein were identified and used to determine the chromosomal location of the corresponding gene (termedIFI-78K gene) by hybridization to DNA from a panel of human x rodent somatic cell hybrids. The newly identified gene is located on chromosome 21. This has been confirmed by the observation of a gene dosage effect using chromosome 21 trisomic cells (fibroblasts derived from Down's syndrome patients). Among all interferon-inducible genes mapped so far, theIFI-78K gene is the only one located on chromosome 21, together with the gene for the receptor of type I interferon. Our results also provide further evidence for homology between human chromosome 21 and mouse chromosome 16, since the gene encoding the mouse Mx protein (the presumed mouse homolog protein of human p78 protein) has been assigned to chromosome 16.

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Authors and Affiliations

  1. Pharmaceuticals Research, Ciba-Geigy Ltd., CH-4002, Basle, Switzerland

    Michel A. Horisberger, Marc Wathelet, Josiane Szpirer, Claude Szpirer, Quamrul Islam, Göran Levan, Georges Huez & Jean Content

  2. Laboratorie de Chimie Biologique, Université Libre de Bruxelles, B-1640, Rhode St-Genèse

    Marc Wathelet & Georges Huez

  3. Laboratorie de Génétique, Université Libre de Bruxelles, B-1640, Rhode St-Genèse

    Josiane Szpirer

  4. Laboratorie de Biologie du Développement, Université Libre de Bruxelles, B-1640, Rhode St-Genèse

    Claude Szpirer

  5. Department of Genetics, University of Göteborg, S-40033, Göteborg

    Quamrul Islam & Göran Levan

  6. Institut Pasteur du Brabant, B-1180, Bruxelles

    Jean Content

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  1. Michel A. Horisberger
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  2. Marc Wathelet
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  6. Göran Levan
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  7. Georges Huez
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  8. Jean Content
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Horisberger, M.A., Wathelet, M., Szpirer, J. et al. cDNA cloning and assignment to chromosome 21 ofIFI-78K gene, the human equivalent of murineMx gene. Somat Cell Mol Genet 14, 123–131 (1988). https://doi.org/10.1007/BF01534397

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  • DOI: https://doi.org/10.1007/BF01534397

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