Abstract
Cells ofRhodopseudomonas spheroides were depigmented by aerobic growth in the light and then transferred to 4% oxygen in the dark to induce pigment synthesis. Pigment synthesis and photochemical activity were measured fluorometrically. In conjunction with the fluorescence studies, thylakoid morphogenesis was followed by electron microscopy of thin sections of cells fixed during the repigmentation process.
Both bacteriochlorophyli and the onset of photochemical activity were detected before distinct thylakoids were observed. Subsequent bacteriochlorophyll synthesis was associated with a gradual increase in the thylakoid content throughout the developmental process.
The results obtained strongly indicate that initially the cytoplasmic membrane is modified by pigment incorporation, possibly at specific sites, and that the bacteriochlorophyll is photochemically active in the pigmented cytoplasmic membrane or in the early stages of invagination.
Finally, in a confirmation of previous hypotheses, these studies provide evidence for the origin of the thylakoids as a protrusion and invagination of the cytoplasmic membrane. This is followed by constriction and subsequent proliferation and branching to form a continuous membrane system which gives rise to chromatophores upon cellular disruption.
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References
C. B. van Niel,Bact. Rev.,8 (1944) 1.
G. Cohen-Bazire, W. R. Sistrom and R. Y. Stanier,J. Cellular Comp. Physiol.,49 (1957) 25.
R. A. Cellarius and G. A. Peters,Biochim. Biophys. Acta,189 (1969) 234.
R. A. Cellarius and G. A. Peters,Photochem. Photobiol.,7 (1968) 325.
J. Oelze, M. Biedermann, E. Freund-Mölbert and G. Drews,Arch. Mikrobiol.,66 (1969) 154.
A. Gorchein, A. Neuberger and G. H. Tait,Proc. Roy. Soc. (London), B.,171 (1968) 111.
M. Biedermann, G. Drews, R. Marx and J. Schröder,Arch. Mikrobiol.,56 (1967) 133.
J. Lascelles,Biochem. J.,72 (1959) 508.
W. R. Sistrom,J. Gen. Microbiol.,28 (1962) 599.
W. R. Sistrom,J. Gen. Microbiol.,28 (1962) 607.
K. D. Gibson, A. Neuberger and G. H. Tait,Biochem. J.,83 (1962) 539.
K. D. Gibson, A. Neuberger and G. H. Tait,Biochem. J.,88 (1963) 325.
J. Lascelles and J. F. Szilagyi,J. Gen. Microbiol.,38 (1965) 55.
E. Gray,Biochim. Biophys. Acta,138 (1967) 550.
J. J. Ferretti and E. D. Gray,J. Bacteriol.,95 (1968) 1400.
G. Cohen-Bazire and R. Kunisawa,J. Cell Biol.,16 (1963) 401.
E. S. Boatman,J. Cell Biol.,20 (1964) 297.
M. C. Karunairatnam, J. Spizizen and H. Gest,Biochim. Biophys. Acta,29 (1958) 649.
S. C. Holt and A. G. Marr,J. Bacteriol. 89 (1965) 1402.
D. D. Hickman and A. W. Frenkel,J. Cell Biol.,25 (1965) 279.
J. Oelze, M. Biedermann and G. Drews,Biochim. Biophys. Acta,173 (1969) 436.
J. Oelze and G. Drews,Biochim. Biophys. Acta,173 (1969) 448.
H. K. Schachman, A. B. Pardee and R. Y. Stanier,Arch. Biochem. Biophys.,38 (1952) 245.
A. E. Vatter and R. S. Wolfe,J. Bacteriol.,75 (1958) 480.
A. Gorchein, A. Neuberger and G. H. Tait,Proc. Roy. Soc. (London), B,170 (1968) 224.
P. B. Worden and W. R. Sistrom,J. Cell Biol.,23 (1964) 135.
K. D. Gibson,J. Bacteriol.,90 (1965) 1059.
M. Sporn, T. Wanko and W. Dingman,J. Cell Biol.,15 (1962) 109.
G. A. Peters, Thesis, Univ. of Mich., 1970.
G. Millonig,J. Biophys. Biochem. Cytol.,11, (1961) 736.
G. Cohen-Bazire and R. Kunisawa,Proc. Nat. Acad. Sci. U.S.,46 (1960) 1543.
Th. Förster,Disc. Faraday Soc.,27 (1959) 7.
A. Gorchein,Proc. Roy. Soc. (London), B,170 (1968) 247.
S. C. Holt and A. G. Marr,J. Bacteriol.,89 (1965) 1421.
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Extracted in part from the doctoral thesis of G. A. Peters submitted to the University of Michigan in partial fulfillment of the requirements for the Ph.D. degree. For paper I of this series see reference [3].
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Peters, G.A., Cellarius, R.A. Photosynthetic membrane development inRhodopseudomonas spheroides . J Bioenerg Biomembr 3, 345–359 (1972). https://doi.org/10.1007/BF01516074
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DOI: https://doi.org/10.1007/BF01516074