Summary
A protocol is described for the delivery of plasmid DNA into plant protoplasts by means of electroporation. Expression of the DNA is monitored 24 h later by a “transient assay” for chloramphenicol acetyltransferase (CAT). Stable transformants are recovered by selecting for kanamycin resistance using a modified version of the agarose-bead technique.
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Bates, G.W. Electroporation of protoplasts. Journal of Tissue Culture Methods 12, 121–126 (1989). https://doi.org/10.1007/BF01404437
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DOI: https://doi.org/10.1007/BF01404437