Summary
Family studies were carried out in a population sample from north west Germany using 4 amplifiable VNTR polymorphic systems D1S80 (MCT118), ApoB, D17S30 (YNZ22) and COL2A1. Separation was carried out in polyacrylamide gels and visualised using silver staining. In family studies (n = 30) no evidence of new mutations was found. The population study of unrelated individuals (mothers and putative fathers) showed that all 4 systems were highly polymorphic and similar to other population studies. The combined exclusion chance was calculated to be approximately 99% and the combined discrimination index 1.5 · 10−4. The HardyWeinberg equilibrium was checked by forming groups of alleles and no significant deviations could be found in all systems.
Zusammenfassung
Untersucht wurden Familien einer nordwestdeutschen Bevölkerungsstichprobe mit 4 amplifizierbaren VNTR-Polymorphismen (MCT118, ApoB, YNZ22, COL2A1). Die Darstellung der Systeme erfolgte in Polyacrylamidgelen durch Silberfärbung. Es fand sich kein Hinweis auf Neumutationen. Anhand der typisierten Unverwandten (Mütter und Putativväter) konnten für jedes System Allelfrequenzen ermittelt werden, die im Vergleich zu anderen Bevölkerungsstichproben relativ gute Übereinstimmungen aufwiesen. Die Berechnung der kombinierten Ausschlußchance für die 4 Systeme führte zu einem Wert von etwa 99%. Der dazu korrespondierende Diskriminationsindex betrug 1,5 · 10−4. Zur Uberprüfung des Hardy-Weinberg-Gleichgewichts wurden Allelgruppen gebildet. Für alle 4 Systeme konnte das Hardy-Weinberg-Gleichgewicht nachgewiesen werden.
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Rand, S., Puers, C., Skowasch, K. et al. Population genetics and forensic efficiency data of 4 AMPFLP's. Int J Leg Med 104, 329–333 (1992). https://doi.org/10.1007/BF01369552
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DOI: https://doi.org/10.1007/BF01369552