Summary
The antineuraminidase (AN) antibody response to vaccination of chickens with intact virus and different subunit preparations of the influenza virus strains A/Sing/1/57 (H2N2), A/Hong Kong/1/68 (H3N2) and A/Pt. Chalmers/1/73 (H3N2) was tested comparatively.
Using a photometric method capable of analysing mixtures of AN antibodies against antigenically different N2 neuraminidases, it was concluded that vaccination with subunits produced by treatment with bromelain and Sarkosyl can yield AN antibody response against heterologous neuraminidase. By contrast, vaccination with intact and ether-treated virus gave AN antibody response against homologous neuraminidase, only.
These findings were confirmed when testing sera by means of enzyme inhibition test and by adsorption experiments with homologous and heterologous neuraminidases.
The conclusion was reached that the NA's of the strains A/Sing/1/57 and A/Pt. Chalmers/1/73 share antigenic determinants and that the NA of the strain A/Hong Kong/1/68 shares antigenic determinants with that of the strains A/Sing/1/57 and A/Pt. Chalmers/1/73.
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Abbreviations
- ACU:
-
antibody concentration unit
- AN:
-
antineuraminidase
- HA:
-
hemagglutinin
- HCU:
-
hemagglutinin concentration unit
- mU:
-
micro units
- NA:
-
neuraminidase
- NIT:
-
neuraminidase inhibition test
- PBS:
-
0.15 M NaCl solution buffered at pH 7.0 with 0.01 M phosphate
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A portion of the experiments described in this paper has been presented at the International Symposium on Animal and Human Influenzas, Alfort, September 1979.
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Drescher, J., Desselberger, U. Antineuraminidase antibody response to vaccination of chickens with intact virus and different subunit preparations of the influenza virus strains A/Sing/1/57 (H2N2), A/Hong Kong/1/68 (H3N2) and A/Port Chalmers/1/73 (H3N2). Archives of Virology 64, 235–246 (1980). https://doi.org/10.1007/BF01322703
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DOI: https://doi.org/10.1007/BF01322703