Summary
The iridoviruses fromWiseana cervinata (WIV, type 9),Costelytra zealandica (CzIV, type 16) andChilo suppressalis (CIV, type 6) were compared by SDS-PAGE and Western protein blotting for antigenic determinants. The major capsid proteins were isolated and oligonucleotide probes were synthesized from the partial amino acid sequences. The DNA regions coding for the major capsid proteins of WIV (VP52), CzIV (VP53) and CIV (VP50) were located by hybridization of the oligonucleotide probes to blots of the viral DNA. The major capsid protein was used as the zero point for the proposed linearized maps of these viruses.
Using antibody and125I-labelling, several proteins were identified as being on the surface of the virion. It was also shown that CIV was not as antigenically distinct from these two viruses as previously reported.
References
Fukaya M, Nasu S (1966) AChilo iridescent virus (CIV) from the rice stem borerChilo suppressalis. Appl Ent Zool 1: 69–76
Kalmakoff J, Robertson JS (1970) Serological relationships ofWiseana iridescent virus (WIV) to other iridescent viruses. Proc Univ Otago Med Sch 48: 16–18
Kalmakoff J, Moore S, Pottinger RP (1972) An iridescent virus from the grass grubCostelytra zealandica: Serological study. J Invertebr Pathol 20: 70–76
Elliot RM, Lescott T, Kelly DC (1977) Serological relationship of an iridescent virus (type 25) recently isolated fromTipula sp. with two other iridescent viruses (types 2 and 22). Virology 81: 309–316
Kelly DC, Ayers MD, Lescott T, Robertson JS, Happ GM (1979) A small iridescent virus (type 29) isolated fromTenebrio molitor: a comparison of its proteins and antigens with six other iridescent viruses. J Gen Virol 42: 95–105
Willis DB, Goorha R, Miles M, Granoff A (1977) Macromolecular synthesis in cells infected by frog virus 3. VII. Transcriptional and post-translational control of gene expression. J Virol 24: 326–342
Black PN, Blair CD, Butcher A, Capinera JL, Happ GM (1981) Biochemistry and ultrastructure of iridescent virus type 29. J Invertebr Pathol 38: 12–21
Moore NF, Kelly DC (1980) A comparative study of the polypeptides of three iridescent viruses by N-terminal analysis, amino acid analysis and surface labelling. J Invertebr Pathol 36: 415–422
Tripier-Darcy F, Braunwald J, Kirn A (1982) Localisation of some frog virus 3 structural polypeptides. Virology 116: 635–640
Tajbakhsh S, Lee PE, Watson DC, Seligy VL (1990) Molecular cloning, characterisation, and expression of theTipula iridescent virus capsid gene. J Virol 64: 125–136
McMillan NAJ, Davison S, Kalmakoff J (1990) Comparison of the genomes of two sympatric iridescent viruses. Arch Virol 114: 277–285
Kelly DC, Avery RF (1974) The DNA content of four small iridescent viruses: Genome size, redundancy, and homology determined by renaturation kinetics. Virology 57: 425–435
Kelly DC (1985) Insect iridescent viruses. Curr Top Microbiol Immunol 116: 23–35
Stanley P, Haslam EA (1971) The polypeptides of influenza virus. V. Localisation of the polypeptides in the virion by iodination techniques. Virology 46: 764–773
Kalmakoff J, McMillan N, Davison S (1990) Insect iridescent virus types 9 and 16. In: Darai G (ed) Molecular biology of iridoviruses. Kluwer, Boston, pp 113–136
Devauchelle G, Attias J, Monnier C, Barray S, Cerutti M, Guerillon J, Orange-Balange N (1985)Chilo iridescent virus. Curr Top Microbiol Immunol 116: 37–48
Cerutti M, Devauchelle G (1985) Characterisation and localisation of CIV polypeptides. Virology 145: 123–131
Cochran MA, Faulkner P (1983) Location of homologous DNA sequences interspersed at five regions in the baculovirus AcMNPV genome. J Virol 45: 961–970
Ward VK, Kalmakoff J (1987) Physical mapping of the DNA genome of the insect iridescent virus type 9 fromWiseana spp. larvae. Virology 160: 507–510
Fischer M, Schnitzler P, Delius H, Rosen-Wolff A, Darai G (1990) Molecular biology of iridescent virus type 6. In: Darai G (ed) Molecular biology of iridoviruses. Kluwer, Boston, pp 45–80
Cameron IR (1990) Identification and characterisation of the gene encoding the major structural protein of the insect iridescent virus type 22. Virology 178: 35–42
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Davison, S., Carne, A., McMillan, N.A.J. et al. A comparison of the structural polypeptides of three iridescent viruses (types 6, 9, and 16) and the mapping of the DNA region coding for their major capsid polypeptides. Archives of Virology 123, 229–237 (1992). https://doi.org/10.1007/BF01317153
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01317153