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Preliminary comparisons of the biological properties of two strains of feline immunodeficiency virus (FIV) isolated in Japan with FIV Petaluma strain isolated in the United States

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Summary

Two strains of feline immunodeficiency virus (FIV) were isolated directly from peripheral blood mononuclear cells (PBMCs) of Japanese domestic cats or indirectly from PBMCs of specific pathogen free (SPF) cats inoculated with whole blood from naturally infected cats with FIV by cocultivation with primary PBMCs from SPF cats. Two isolates, designated as FIV TM 1 and FIV TM 2, had a lentivirus-like morphology by elecron microscopy, a tropism for interleukin-2 dependent T-lymphocytes and Mg2+-dependent reverse transcriptase activity. By immunoblotting the isolates gave bands at 130, 48, 44, 40, 28, 17, and 13 kDa, and these bands except 130 kDa were detected in FIV Petaluma strain when reacted with the plasma of cats infected naturally with FIV TM 1 strain.

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Authors and Affiliations

  1. Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Tokyo

    Takayuki Miyazawa, Tetsuya Furuya, Yukinobu Tohya, Eiji Takahashi & Takeshi Mikami

  2. Department of Biomedical Science, Faculty of Agriculture, University of Tokyo, Tokyo

    Shin-ichi Itagaki

  3. Department of Laboratory Animal Science, School of Medicine, Kitasato University, Sagamihara, Japan

    Kenji Nakano

Authors
  1. Takayuki Miyazawa
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  2. Tetsuya Furuya
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  3. Shin-ichi Itagaki
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  4. Yukinobu Tohya
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  5. Kenji Nakano
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  6. Eiji Takahashi
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  7. Takeshi Mikami
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Miyazawa, T., Furuya, T., Itagaki, Si. et al. Preliminary comparisons of the biological properties of two strains of feline immunodeficiency virus (FIV) isolated in Japan with FIV Petaluma strain isolated in the United States. Archives of Virology 108, 59–68 (1989). https://doi.org/10.1007/BF01313743

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  • DOI: https://doi.org/10.1007/BF01313743

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