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Analysis of the genetic diversity of genes 5 and 6 among group C rotaviruses using cDNA probes

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Summary

Two partial cDNA clones of genes 5 (encoding the major inner capsid protein VP 6) and 6 (encoding a nonstructural protein) of the porcine group (Gp) C rotavirus (Cowden strain) were radiolabeled with32P and used individually as probes in Northern and dot blot hybridization assays. The specificity of each probe was tested against genomic dsRNA from: (1) porcine Gp A, B, and C rotaviruses; (2) Gp C rotaviruses from different species; and (3) porcine Gp C rotavirus field strains with varying electropherotype patterns. Neither probe hybridized with ds RNA from the porcine Gp A and B strains under the stringency conditions employed in the study. However, the gene 5 probe hybridized with the corresponding gene from the homologous porcine and the heterologous human and bovine Gp C rotaviruses tested. The gene 6 probe hybridized with the corresponding gene from the homologous Cowden strain, but hybridized weakly with gene 6 from the human and bovine Gp C rotaviruses. Both probes recognized all six different porcine Gp C field strains, although with varying intensities. Our results demonstrate that the gene 5 and 6 probes used in this study are specific for Gp C rotaviruses. However, evidence for greater genetic variation in the gene 6 among porcine, bovine and human Gp C strains suggested that the gene 5 probe may prove more broadly reactive among Gp C strains from different species. cDNA probes used in our study should prove useful for the detection of Gp C rotaviruses in feces and facilitate epidemiologic studies.

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Authors and Affiliations

  1. Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, Ohio

    B. M. Jiang, H. Tsunemitsu & Linda J. Saif

  2. Laboratory of Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA

    Y. Qian & K. Y. Green

  3. Ehime Prefectural Institute of Public Health, Matsuyama, Ehime, Japan

    M. Oseto & Y. Yamashita

Authors
  1. B. M. Jiang
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  2. H. Tsunemitsu
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  3. Y. Qian
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  4. K. Y. Green
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  5. M. Oseto
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  6. Y. Yamashita
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  7. Linda J. Saif
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Jiang, B.M., Tsunemitsu, H., Qian, Y. et al. Analysis of the genetic diversity of genes 5 and 6 among group C rotaviruses using cDNA probes. Archives of Virology 126, 45–56 (1992). https://doi.org/10.1007/BF01309683

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  • DOI: https://doi.org/10.1007/BF01309683

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