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Taurine infused intrastriatally elevates, but intranigrally decreases striatal extracellular dopamine concentration in anaesthetised rats

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Summary

In the present study we infused taurine (50,150 or 450mM, 2μl/min for 4h) into the dorsal striatum or into the substantia nigra via microdialysis probe and estimated the extracellular concentrations of dopamine and its metabolites, 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA), in the dorsal striatum of anaesthetised rats. Intrastriatal infusion of taurine elevated striatal dopamine at all concentrations studied. At the 450 mM concentration taurine elevated the extracellular dopamine 10-fold, but only in the first 30min sample after starting the taurine infusion. At 50 and 150mM taurine elevated dopamine throughout the 4h infusion maximally up to 3–4-fold the control level. Extracellular DOPAC was increased by 150 and 450mM taurine (up to about 150–160% of the control level), whereas at all three concentrations taurine decreased HVA to about 85% of the control; however, the decrease caused by 450mM taurine was short-lasting. At all three concentrations taurine infused into the substantia nigra decreased the extracellular dopamine in the ipsilateral striatum to about 40–50% of the control, and increased extracellular DOPAC and HVA maximally to about 150% and 170% of the control, respectively. These results show that the effects of taurine on the concentrations of extracellular dopamine and its metabolites depend on its administration site on nigrostriatal dopaminergic neurons. It elevates the extracellular dopamine when given into the striatum, but when given into the cell body region of the nigrostriatal dopaminergic pathway it decreases the extracellular dopamine in the ipsilateral striatum.

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References

  • Ahtee L, Vahala M-L (1985) Taurine and its derivatives alter brain dopamine metabolism similarly to GABA in mice and rats. In: Oja S, Ahtee L, Kontro P, Paasonen MK (eds) Taurine: biological actions and clinical perspectives. Alan R Liss, New York, pp 331–341

    Google Scholar 

  • Billard JM (1990) Taurine in deep cerebellar nuclei of the rat. In vivo comparison to GABA inhibitory effect. Brain Res 514: 155–158

    Google Scholar 

  • Bureau MH, Olsen RW (1991) Taurine acts on a subclass of GABAA receptors in mammalian brain in vitro. Eur J Pharmacol 27: 9–16

    Google Scholar 

  • Butcher SP, Hamberger A (1987) In vivo studies on the extracellular, and veratrine-releasable, pools of endogenous amino acids in the rat striatum: effects of corticostriatal deafferentation and kainic acid lesion. J Neurochem 48: 713–721

    Google Scholar 

  • Clarke DJ, Smith AD, Bolam JP (1983) Uptake of [3H]-taurine into medium-size neurons and into identifed striatonigral neurons in the rat neostriatum. Brain Res 289: 342–348

    Google Scholar 

  • Crawford JM, Curtis DR (1964) The excitation and depression of mammalian cortical neurones by amino acids. Br J Pharmacol 23: 313–329

    Google Scholar 

  • Davies J, Dray A (1976) Changes in amino acid sensitivity during polypeptide “desensitisation”. Nature 262: 606–607

    Google Scholar 

  • Della Corte L, Clarke DJ, Bolam JP, Smith AD (1987) Uptake, localization and release of taurine in rat basal ganglia. In: Huxtable RJ, Franconi F, Giotti A (eds) The biology of taurine. Plenum, New York, pp 285–294

    Google Scholar 

  • Della Corte L, Bolam JP, Clarke DJ, Parry DM, Smith AD (1990) Sites of [3H]taurine uptake in the rat substantia nigra in relation to the release of taurine from the striatonigral pathway. Eur J Neurosci 2: 50–61

    Google Scholar 

  • Dolora P, Franconi F, Giotti A, Basoni R, Valensin G (1978) Taurine-calcium interaction measured by means of (13C) nuclear magnetic resonance. Biochem Pharmacol 27: 803–804

    Google Scholar 

  • Dray A, Straughan DW (1976) Synaptic mechanisms in the substantia nigra. J Pharm Pharmacol 28: 400–405

    Google Scholar 

  • Farrant M, Webster RA (1989) Compartmental distribution of endogenous amino acids in the substantia nigra of the rat. Brain Res 480: 344–348

    Google Scholar 

  • Garcia de Yebenes Prous J, Carlsson A, Mena Gomez MA (1978) The effect of taurine on motor behaviour, body temperature and monoamine metabolism in rat brain. Naunyn Schmiedebergs Arch Pharmacol 304: 95–99

    Google Scholar 

  • Giorguieff MF, Kemel ML, Glowinski J, Besson MJ (1978) Stimulation of dopamine release by GABA in rat striatal slices. Brain Res 139: 115–130

    Google Scholar 

  • Huxtable RJ (1989) Taurine in the central nervous system and the mammalian actions of taurine. Prog Neurobiol 32: 471–533

    Google Scholar 

  • Häusser MA, Yung WH, Lacey MG (1992) Taurine and glycine activate the same Cl conductance in substantia nigra dopamine neurones. Brain Res 571: 103–108

    Google Scholar 

  • Kerwin R, Pycock C (1979) Effect of ω-amino acids on tritiated dopamine release from rat striatum: evidence for a possible glycinergic mechanism. Biochem Pharmacol 28: 2193–2197

    Google Scholar 

  • Kito S, Shimoyama M, Arakawa R (1986) Effects of neurotransmitters or drugs on the in vivo release of dopamine and its metabolites. Jpn J Pharmacol 40: 57–67

    Google Scholar 

  • Kontro P, Oja SS (1988) Release of taurine, GABA and dopamine from rat striatal slices, mutual interaction and developmental aspects. Neuroscience 24: 49–58

    Google Scholar 

  • Kontro P, Oja SS (1990) Interactions of taurine with GABAB binding sites in mouse brain. Neuropharmacology 29: 243–247

    Google Scholar 

  • Korf J, Venema K (1983) Amino acids in the substantia nigra of rats with striatal lesions produced by kainic acid. J Neurochem 40: 1171–1173

    Google Scholar 

  • Lombardini JB (1976) Regional and subcellular studies on taurine in the rat central nervous system. In: Huxtable R, Barbeau A (eds) Taurine. Raven Press, New York, pp 311–326

    Google Scholar 

  • López-Colomé AM, Pasantes-Morales H (1981) Taurine binding to membrane from rat brain regions. J Neurosci Res 6: 475–485

    Google Scholar 

  • Mäkinen M, Ahtee L (1994) Intrastriatal taurine increases dopamine release in a tetrodotoxin sensitive manner. Can J Physiol Pharmacol 72 [Suppl 1]: 425

    Google Scholar 

  • Mäkinen M, Pitkänen M, Lillsunde P, Seppälä T, Ahtee L (1993) Effects of taurine on striatal dopamine release differ after intrastriatal and intranigral infusions. Naunyn Schmiedebergs Arch Pharmacol 347: 135R

    Google Scholar 

  • O'Neill R (1986) Effects of intranigral injection of taurine and GABA on striatal dopamine release monitored voltammetrically in the unanaesthetized rat. Brain Res 382: 28–32

    Google Scholar 

  • Palkovits M, Banay-Schwartz M, Lathja A (1990) Taurine levels in brain nuclei of young adult and aging rats. In: Pasantes-Morales H, Shain W, Martin DL, Martin del Rio R (eds) Taurine, functional neurochemistry, physiology, and cardiology. Wiley & Liss, New York, pp 45–51

    Google Scholar 

  • Panula-Lehto E, Mäkinen M, Ahtee L (1992) Effects of taurine, homotaurine and GABA on hypothalamic and striatal dopamine metabolism. Naunyn Schmiedebergs Arch Pharmacol 346: 57–62

    Google Scholar 

  • Paxinos G, Watson C (1986) The rat brain in stereotaxic coordinates. Academic Press, New York

    Google Scholar 

  • Reid M, Herrera-Marschitz M, Hökfelt T, Terenius L, Ungerstedt U (1988) Differential modulation of striatal dopamine release by intranigral injection of γ-aminobutyric acid (GABA), dynorphin A and substance P. Eur J Pharmacol 147: 411–420

    Google Scholar 

  • Reid MS, Herrera-Marschitz M, Hökfelt T, Lindefors N, Persson H, Ungerstedt U (1990) Striatonigral GABA, dynorphin, substance P and neurokinin A modulation of nigrostriatal dopamine release evidence for direct regulatory mechanisms. Exp Brain Res 82: 293–303

    Google Scholar 

  • Santiago M, Westerink BHC (1992) The role of GABA receptors in control of nigrostriatal dopaminergic neurons, dual-probe microdialysis study in awake rats. Eur J Pharmacol 219: 175–181

    Google Scholar 

  • Semba J, Kito S, Toru M (1995) Characterisation of extracellular amino acids in striatum of freely moving rats by in vivo microdialysis. J Neural Transm 100: 39–52

    Google Scholar 

  • Simmonds MA (1986) Classification of inhibitory amino acid receptors in the mammalian nervous system. Med Biol 64: 301–311

    Google Scholar 

  • Smolders I, De Klippel N, Sarre S, Ebinger G, Michotte Y (1995) Tonic GABA-ergic modulation of striatal dopamine release studied by in vivo microdialysis in the freely moving rat. Eur J Pharmacol 284: 83–91

    Google Scholar 

  • Spears RM, Martin DL (1982) Resolution and brain regional distribution of cysteine sulfinate decarboroxylase isoenzymes from hog brain. J Neuchem 38: 985–991

    Google Scholar 

  • Tossman U, Jonsson G, Ungerstedt U (1986) Regional distribution and extracellular levels of amino acids in rat central nervous system. Acta Physiol Scand 127: 533–545

    Google Scholar 

  • Westerink BHC (1985) Sequence and significance of dopamine metabolism in the rat brain. Neurochem Int 2: 221–227

    Google Scholar 

  • Westerink BHC, Damsma G, Rollema H, De Vries JB, Horn AS (1987) Scope and limitations of in vivo brain dialysis: a comparison of its application to various neurotransmitter systems. Life Sci 41: 1763–1776

    Google Scholar 

  • Westerink BHC, Santiago M, De Vries JB (1992a) The release of dopamine from nerve terminals and dendrites of nigrostriatal neurons induced by excitatory amino acids in conscious rat. Naunyn Schmiedebergs Arch Pharmacol 345: 523–529

    Google Scholar 

  • Westerink BHC, Santiago M, De Vries JB (1992b) In vivo evidence for a concordant response of terminal and dendritic dopamine release during intranigral infusion of drugs. Naunyn Schmiedebergs Arch Pharmacol 346: 637–643

    Google Scholar 

  • Yadid G, Pacak K, Golomb E, Harvey-White JD, Lieberman DM, Kopin IJ, Goldstein DS (1993) Glycine stimulates striatal dopamine release in conscious rats. Br J Pharmacol 110: 50–53

    Google Scholar 

  • Zetterström T, Sharp T, Collin AK, Ungerstedt U (1988) In vivo measurement of extracellular dopamine and DOPAC in rat striatum after various dopamine-releasing drugs; implications for the origin of extracellular DOPAC. Eur J Pharmacol 148: 327–334

    Google Scholar 

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Ruotsalainen, M., Heikkilä, M., Lillsunde, P. et al. Taurine infused intrastriatally elevates, but intranigrally decreases striatal extracellular dopamine concentration in anaesthetised rats. J. Neural Transmission 103, 935–946 (1996). https://doi.org/10.1007/BF01291784

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  • DOI: https://doi.org/10.1007/BF01291784

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