Summary
Primary roots of maize seedlings have been treated with solutions of lanthanum and lead salts in an attempt to demonstrate endocytosis. Subsurface cells in the root cap reveal deposits of these heavy metals in coated pits in the plasma membrane and in coated vesicles. In addition lead deposits were observed in coated evaginations (pits) on large (secretory) vesicles present at the trans-pole of the Golgi apparatus and on small vacuoles. Lead was also found in the peripheral regions of individual cisternae throughout the dictyosomal stack. We interpret our results as providing evidence for coated pit/coated vesicle-mediated endocytosis and for the direct recycling of plasma membrane to the Golgi apparatus.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aicken, R. M., Dean, A. C. R., Cheetham, A. K., Skarnulis, A. J., 1979: Electron microscope studies on the uptake of lead by aCitrobacter species. Microbiol. Letters9, 7–15.
Altstiel, L., Branton, D., 1983: Fusion of coated vesicles with lysosomes: measurement with a fluorescence assay. Cell32, 921–929.
Bonnett, H. T., Newcomb, E. H., 1966: Coated vesicles and other cytoplasmic components of growing root hairs of radish. Protoplasma62, 59–75.
Bretscher, M. S., Pearse, B. M. F., 1984: Coated pits in action. Cell38, 3–4.
Brown, M. S., Anderson, R. G. W., Goldstein, J. L., 1983: Recycling receptors: the roundtrip itinerary of migrant membrane proteins. Cell32, 663–667.
Carpita, N., Sabularse, D., Montezinos, D., Delmer, D. P., 1979: Determination of the pore size of cell walls of living plant cells. Science205, 1144–1147.
Cram, W. J., 1980: Pinocytosis in plants. New Phytol.84, 1–17.
Deurs, B. van, Nilausen, K., Faergeman, O., Meinatz, H., 1982: Coated pits and pinocytosis of cationized ferritin in human skin fibroblasts. Eur. J. Cell Biol.27, 270–278.
Doohan, M. E., Palevitz, B. A., 1980: Microtubules and coated vesicles in guard-cell protoplasts ofAllium cepa L. Planta149, 389–401.
Dupont, F. M., Leonard, R. T., 1977: The use of lanthanum to study the functional development of the Casparian strip in corn roots. Protoplasma91, 315–323.
Evert, R. F., Botha, C. E. J., Mierzwa, R. J., 1985: Free-space marker studies on the leaf ofZea mays L. Protoplasma126, 62–73.
Franke, W. W., Herth, W., 1974: Morphological evidence fordenovo formation of plasma membrane from coated vesicles in exponentially growing cultivated plant cells. Exp. Cell Res.89, 447–451.
Goud, B., Huet, C, Louvard, D., 1985: Assembled and unassembled pools of clathrin: a quantitative study using an enzyme immunoassay. J. Cell Biol.100, 521–527.
Herzog, V., 1981: Pathways of endocytosis in secretory cells. Trends in biochem. Sci.6, 319–322.
Hopkins, C., 1983: The importance of the endosome in intracellular traffic. Nature304, 684–685.
Joachim, S., Robinson, D. G., 1984: Endocytosis of cationic ferritin by bean leaf protoplasts. Eur. J. Cell Biol.34, 212–216.
Lane, S. D., Martin, E. S., 1982: An ultrastructural examination of lead localization in germinating seeds ofRaphanus sativus. Z. Pflanzenphysiol.107, 33–40.
Malone, C, Koeppe, D. E., Miller, R. J., 1974: Localization of lead accumulated by corn plants. Plant Physiol.53, 388–394.
Mollenhauer, H. H., Morré, D. J., 1976: Cytochalasin B, but not colchicine, inhibits migration of secretory vesicles in root tips of maize. Protoplasma87, 39–48.
Nagahashi, G., Thomson, W. W., Leonard, R. T., 1974: The casparian strip as a barrier to the movement of lanthanum in corn roots. Science183, 670–671.
Newcomb, E. H., 1980: Coated vesicles: their occurence in different plant cell types. In: Coated Vesicles (Ochleford, C. D., Whyte, A., eds.), pp. 55–68. Cambridge: Cambridge University Press.
Pearse, B. M. F., Bretscher, M. S., 1981: Membrane recycling by coated vesicles. Ann. Rev. Biochem.50, 85–101.
Pesacreta, T. C., Lucas, W. J., 1985: Presence of a partially-coated reticulum and a plasma membrane coat in angiosperms. Protoplasma125, 173–184.
Pilzzolato, T. D., Burbano, J. L., Berlin, J. D., Morey, P. R., Pease, R. W., 1976: An electron microscope study of the path of water movement in transpiring leaves of cotton (Gossypium hirsutum L.). J. exp. Bot.27, 145–166.
Revel, J. P., Karnovsky, M. J., 1967: Hexagonal arrays of subunits in intercellular junctions of the mouse heart and liver. J. Cell Biol.20, 313–332.
Robards, A. W., Robb, M., 1972: Uptake and binding of uranyl ions by barley roots. Science178, 980–982.
— —, 1974: The entry of ions and molecules into roots: an investigation using electron-opaque tracers. Planta120, 1–12.
Roth, T. F., Cutting, J. A., Atlas, S. B., 1976: Protein transport: a selective membrane mechanism. J. supramolec. Struct.4, 527–548.
Rothman, J. E., 1981: The Golgi apparatus: two organelles in tandem. Science213, 1212–1219.
Ryser, U., 1979: Cotton fibre differentiation: Occurrence and distribution of coated and smooth vesicles during primary and secondary wall formation. Protoplasma98, 223–239.
Shannon, T. M., Picton, J. M., Steer, M. W., 1984: The inhibition of dictyosome vesicle formation in higher plant cells by cytochalasin D. Eur. J. Cell Biol.33, 144–147.
Sharpe, V., Denny, P., 1976: Electron microscope studies on the obsorption and localization of lead in leaf tissue ofPotamogeton pectinatus. J. exp. Bot.27, 115–162.
Steinman, R. M., Mellman, I. S., Mueller, W. A., Cohn, Z. A., 1983: Endocytosis and the recycling of plasma membrane. J. Cell Biol.96, 1–27.
Steveninck, R. F. M. van, Steveninck, M. E. van, Chescoe, D., 1976: Intracellular binding of lanthanum in root tips of barley (Hordeum vulgare). Protoplasma90, 89–97.
Tanchak, M. A., Griffing, L. R., Mersey, B. G., Fowke, L. C., 1984: Endocytosis of cationized ferritin by coated vesicles of soybean protoplasts. Planta162, 481–486.
Tanton, T. W., Crowdy, S. H., 1972: Water pathways in higher plants. II. Water pathways in roots. J. exp. Bot.23, 600–618.
Thomson, W. W., Platt, K. A., Campbell, N., 1973: The use of lanthanum to delineate the apoplastic continuum in plants. Cytobios8, 57–62.
Valk, P. van der, Rennie, P. J., Connolly, J. A., Fowke, L. C., 1980: Distribution of cortical microtubules in tobacco protoplasts. An immunofluorescence microscopic and ultrastructural study. Protoplasma105, 27–43.
—,Fowke, L. C., 1981: Ultrastructural aspects of coated vesicles in tobacco protoplasts. Can. J. Bot.59, 1307–1313.
Wheeler, H., Hanchey, P., 1971: Pinocytosis and membrane dilation in uranyl-treated plant roots. Science171, 68–71.
—,Baker, B. L., Hanchey, P., 1972: Pinocytosis in root cap cells exposed to uranyl salts. Amer. J. Bot.59, 858–868.
Zee, S.-Y., O'Brien, T. P., 1972: Vascular transfer cells in the wheat spikelet. Austr. J. biol. Sci.24, 35–49.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hübner, R., Depta, H. & Robinson, D.G. Endocytosis in maize root cap cells. Protoplasma 129, 214–222 (1985). https://doi.org/10.1007/BF01279918
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01279918