Summary
The ultrastructure of chloroplasts ofOcymum basilicum L. leaves has been studied following experimental infection with two different strains of Lucerne Mosaic Virus (LMV-Mi and LMV-Fi) and one strain of cucumber mosaic virus (CMV-YW) and after treatment with 3-amino-1,2,4-triazole (AT). Chloroplast alterations vary in each case: in LMV-Mi infection stromacentre-like structures are visible in the plastid stroma; in LMV-Fi infection the plastid lamellar system gives rise to myelin-like structures. AT-treatment changes the plastid grana into “supergrana” and causes the appearance of granular, electron opaque materials in the plastid stroma, but does not cause the formation of stromacentre-like structures. Since AT seems to block the synthesis of chloroplast Fraction 1 protein and the stromacentre seems to be formed by this protein, it may be supposed that the three different viruses tested and AT act in a different way.
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References
Arnott, H. J., S. W. Rosso, andK. M. Smith, 1969: Modification of plastid ultrastructure in tomato leaf cells infected with Tobacco Mosaic Virus. J. Ultrastruct. Res.27, 149–167.
Backmann, M. D., D. S. Robertson, andC. C. Bowen, 1969: Thylakoid anomalies in relation to grana structure in pigment-deficient mutants ofZea mays. J. Ultrastruct. Res.28, 435–451.
Bartels, P. G., 1965: Effect of Amitrole on ultrastructure of plastids in seedlings. Plant and Cell Physiol.6, 361, 227–230.
—,K. Matsuda, A. Siegel, andT. E. Weier, 1967: Chloroplastic ribosomes formation: inhibition by 3-amino-1,2,4-triazole. Plant Physiol.42, 736–741.
—, andT. E. Weier, 1969: The effect of 3-amino-1,2,4-triazole on the ultrastructure of plastids ofTriticum vulgare seedlings. Amer. J. Bot.56 (1), 1–7.
Belli, G., 1961: Rilievi ed esperienze sulla trasmissione per seme del virus del mosaico dell'erba medica e dimostrazione della sua esclusione in cloni di vite virusati. Ann. Fac. Agraria Milano10, 33–47.
Conti, G. G., and G.Vegetti, in preparation: Descrizione di un ceppo del virus del mosaico della erba medica isolato da Tabacco.
Favali, M. A., andE. Meyer, 1969: Electron microscope investigations on the stromacentre. Giorn. Bot. Ital.103, 189–203.
Gerola, F. M., andM. Bassi, 1966: An electron microscopy study of leaf vein tumours from maize plants experimentally infected with maize rough dwarf virus. Caryologia19, 13–40.
— —, andE. Betto, 1965: Some observations on the shape and localization of different viruses in experimentally infected plants, and on the fine structure of the host cells. I. Arabis mosaic virus inChenopodium amaranticolor. Caryologia18, 353–375.
— — — 1969: A submicroscopical study of leaves of Alfalfa, Basil, and Tobacco experimentally infected with Lucerne Mosaic Virus. Protoplasma67, 307–318.
Guillot-Salomon, T., 1966: The action of 3-amino-1,2,4-triazole on the ultrastructure of corn chloroplasts. C. R. Acad. Sci. (Paris) Ser. D262, 2510–2513.
Gunning, B. E. S., 1965: The fine structure of chloroplast stroma following aldehyde-osmium tetroxide fixation. J. Cell Biol.24, 79–93.
—,M. W. Steer, andM. P. Cochrane, 1968: Occurence, molecular structure, and induced formation of the “stromacentre” in plastids. J. Cell Science3, 445–456.
Herold, F., andJ. Weibel, 1963: Electron microscopic demonstrations of Sugarcane Mosaic Virus particles in cells ofSaccharum officinarum andZea mays. Phytopathology52, 469–471.
Jacobson, A. B., andB. J. Rogers, 1961: Observations concerning the amino-triazole effect on maize chloroplasts using the electron microscope. Plant Physiol.36 (suppl.), xi.
Kitajima, E. W., 1965: Electron microscopy of Vira-Cabeça Virus (Brazilian Tomato spotted wilt virus) within the host cell. Virology26, 89–99.
Orsenigo, M., and G.Marziani, in press: Chloroplast structural changes during temperature-induced bleaching and regreening in a “golden leaf” mutant of Maize.
Price, W. C., 1934: Isolation of some yellow strains of cucumber mosaic. Phytopathology24, 743–761.
Reynolds, E. S., 1963: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol.17, 208–212.
Ridley, S. M., J. P. Thornber, andJ. L. Bailey, 1967: A study of the water-soluble proteins of spinach beet chloroplasts with particular reference Fraction 1 protein. Biochim. biophys. Acta140, 62–79.
Signol, M. M., 1965: Modifications morphologiques, pigmentaires et cytologiques induites chez divers groups de végétaux par quelques substances antichlorophylliennes. Revue Générale de Botanique72, 417–576.
Steer, M. W., B. E. S. Gunning, T. A. Graham, andD. J. Carr, 1968: Isolation, properties, and structures of Fraction 1 protein fromAvenu sativa L. Planta79, 254–267.
Walles, B., 1965: Plastids structures of carotenoid-deficient mutants of sunflower (Helianthus annuus L). I. The white mutant. Hereditas53, 247–256.
Weintraub, M., andH. W. J. Ragetli, 1966: Fine structure of inclusions and organelles inVicia faba infected with Bean Yellow Mosaic Virus. Virology28, 290–302.
Wettstein, D. von, andH. Zech, 1962: The structure of nucleus and cytoplasm in hair cells during Tobacco Mosaic Virus reproduction. Z. Naturforsch.17 b, 376–379.
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This investigation was supported by a grant of C.N.R., Roma.
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Favali, M.A., Conti, G.G. Ultrastructural observations on the chloroplasts of Basil plants either infected with different viruses or treated with 3-amino-1,2,4-triazole. Protoplasma 70, 153–166 (1970). https://doi.org/10.1007/BF01276977
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DOI: https://doi.org/10.1007/BF01276977