Summary
The preprophase-prophase initial aperture (IA) cells ofMarchantia paleacea undergo a particular sequence of protoplasmic changes, which reflects the establishment of an unusual premitotic polarization. The marking feature of preprophase-prophase thallus cells is the shape of the nucleus which becomes spindle-shaped. This phenomenon accompanies the organization of an extranuclear microtubule (MT) sheath, nucleated and/or organized by distinct polar MT organizing centres (MTOCs).
The interphase MTs disappear after activation of polar MTOCs. In preprophase IA cells incomplete preprophase MT bands (PMBs) are organized. They consist of PMB portions which traverse only small portions of the cell cortex at the level of the future cytokinesis and do not form a complete ring. In the same cells other MT bundles, independent of the incomplete PMBs terminate in the cortical cytoplasm abutting on the lower part of the intercellular spaces (ISs) or the surface cavities (SCs). Almost complete or complete PMBs are organized in IA cells in which the plane of PMB formation coincides with that passing through ISs of the same growth.
The observations suggest that in preprophase-prophase IA cells ofMarchantia paleacea cortical MTOCs function in regions distant from each other: One region is the PMB cortical cytoplasm, probably that covering the wall edges, and the other is the one adjacent to the lower part of the wall facing the IS(s) or that underlying the SCs. The competition between the cortical MTOCs as well as between them and the polar ones may be responsible for the organization of incomplete PMBs.
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References
Apostolakos, P., 1980: Ultrastructural studies on the ontogeny of the air pores and air chambers of the liverwortsMarchantia paleacea andLunularia cruciata. Ph.D. Thesis, Athens.
—,Galatis, B., Mitrakos, K., 1982: Studies on the development of the air pores and air chambers ofMarchantia paleacea. I. Light microscopy. Ann. Bot.49, 377–396.
— —, 1985 a: Studies on the development of the air pores and air chambers ofMarchantia paleacea. II. Ultrastructure of the initial aperture formation with particular reference to cortical microtubule organizing centres. Can. J. Bot.63, 744–756.
— —, 1985 b: Studies on the development of the air pores and air chambers ofMarchantia paleacea. IV. Cell plate arrangement in initial aperture cells. Protoplasma128, 136–146.
Bajer, A., Molé-Bajer, J., 1969: Formation of spindle fibers, kinetochore orientation, and behaviour of the nuclear envelope during mitosis in endosperm. Fine structural andin vitro studies. Chromosoma27, 448–484.
— —, 1975: Lateral movements in the spindle and the mechanism of mitosis. In: Molecules and cell movement (Indué, S., Stephens, R. E., eds.), pp. 77–96. New York: Raven Press.
Brown, D. L., Stearns, M. E., Macrae, T. H., 1983: Microtubule organizing centres. In: The cytoskeleton in plant growth and development (Lloyd, C. W., ed.), pp. 55–83. London-New York: Academic Press.
Brown, R. C., Lemmon, B. E., 1982: Ultrastructure of meiosis in the mossRhynchostegium serrulatum. I. Prophasic microtubules and spindle dynamics. Protoplasma110, 23–33.
De Mey, J., Lambert, A. M., Bajer, A. S., Moeremans, M. de, Brabander, M., 1982: Visualization of microtubules in interphase and mitotic plant cells ofHaemanthus endosperm with the immuno-gold staining method. Proc. Nat. Acad. Sci. U.S.A.79, 1898–1902.
Eleftheriou, E. P., 1985: Microtubules and root protophloem ontogeny in wheat. J. Cell Sci. (in press).
Fowke, L. C., Pickett-Heaps, J. D., 1978: Electron microscope study of vegetative cell division in two species ofMarchantia. Can. J. Bot.56, 467–475.
Galatis, B., 1982: The organization of microtubules in guard cell mother cells ofZea mays. Can. J. Bot.60, 1148–1166.
—,Apostolakos, P., 1977: On the fine structure of differentiating mucilage papillae ofMarchantia. Can. J. Bot.55, 772–795.
—,Mitrakos, K., 1979: On the differential divisions and preprophase microtubule bands involved in the development of stomata ofVigna sinensis. J. Cell Sci.37, 11–37.
—,Apostolakos, P., Katsaros, Chr., 1983: Synchronous organization of two preprophase microtubule bands and final cell plate arrangement in subsidiary cell mother cells of someTriticum species. Protoplasma117, 24–39.
— — —, 1984 a: Positional inconsistency between preprophase microtubule band and final cell plate arrangement during triangular subsidiary cell and atypical hair cell formation in twoTriticum species. Can. J. Bot.62, 343–359.
— — —, 1984 b: Experimental studies on the function of the cortical cytoplasmic zone of the preprophase microtubule band. Protoplasma122, 11–26.
— — —,Loukari, H., 1982: Pre-prophase microtubule band and local wall thickening in guard cell mother cells of someLeguminosae. Ann. Bot.50, 779–791.
Gunning, B. E. S., 1981: Microtubules and cytomorphogenesis in a developing organ: The root primordium ofAzolla pinnata. In: Cytomorphogenesis in plants (Kiermayer, O., ed.), pp. 301–325. Wien-New York: Springer.
—, 1983: The cytokinetic apparatus: Its development and spatial regulation. In: The cytoskeleton in plant growth and development (Lloyd, C. W., ed.), pp. 229–292. London-New York: Academic Press.
—,Hardham, A. R., 1982: Microtubules: Ann. Rev. Plant Physiol.33, 651–698.
— —,Hughes, J. E., 1978 a: Pre-prophase bands of microtubules in all categories of formative and proliferative cell divisions inAzolla roots. Planta143, 145–160.
— — —, 1978 b: Evidence for initiation of microtubules in discrete regions of the cell cortex inAzolla root-tip cells, and an hypothesis on the development of cortical arrays of microtubules. Planta143, 161–179.
Heath, B. I., 1978: Experimental studies of mitosis in the fungi. In: Nuclear division in the fungi (Heath, B. I., ed.), pp. 89–176. New York-London: Academic Press.
Hepler, P. K., 1976: The blepharoplast ofMarsilea: itsde novo formation and spindle association. J. Cell Sci.21, 361–390.
—, 1980: Membranes in the mitotic apparatus of barley cells. J. Cell Biol.86, 490–499.
—,Palevitz, B. A., 1974: Microtubules and microfilaments. Ann. Rev. Plant Physiol.25, 309–362.
Jackson, W. T., Doyle, B. G., 1982: Membrane distribution in dividing endosperm cell ofHaemanthus. J. Cell Biol.94, 637–643.
Kallenbach, R. J., Mazia, D., 1982: Origin and maturation of centrioles in association with the nuclear envelope in hypertonicstressed sea urchin eggs. Eur. J. Cell Biol.28, 68–76.
Lambert, A. M., 1980: The role of chromosomes in anaphase trigger and nuclear envelope activity in spindle formation. Chromosoma76, 295–308.
Lehmann, H., Schulz, D., 1969: Elektronenmikroskopische Untersuchungen von Differenzierungsvorgängen bei Moosen. II. Die Zellplatten- und Zellwandbildung. Planta85, 313–325.
Pickett-Heaps, J. D., 1969: The evolution of the mitotic apparatus: an attempts at comparative ultrastructural cytology in dividing plant cells. Cytobios3, 257–280.
—, 1974: Plant microtubules. In: Dynamic aspects of plant ultrastructure (Robards, A. W., ed.), pp. 219–255. London: McGraw-Hill.
—, 1975: Green algae. Structure, reproduction and evolution in selected genera. Sunderland, Massachusetts: Sinauer Associates, Inc., Publishers.
Robbins, R. R., 1984: Origin and behavior of bicentriolar centrosome in the bryophyteRiella americana. Protoplasma121, 114–119.
Roos, U.-P., 1975: Fine structure of an organelle associated with the nucleus and cytoplasmic microtubules in the cellular slime mouldPolysphondylium violaceum. J. Cell Sci.18, 315–326.
Ryan, K. G., 1984: Membranes in the spindle ofIris pollen mother cells during the second division of meiosis. Protoplasma122, 56–67.
Schmiedel, G., Schnepf, E., 1979: Side branch formation and orientation in the caulonema of the moss,Funaria hygrometrica: Normal development and fine structure. Protoplasma100, 367–383.
—,Reiss, H.-D., Schnepf, E., 1981: Association between membranes and microtubules during mitosis and cytokinesis in caulonema tip cells of the mossFunaria hygrometrica. Protoplasma108, 173–190.
Schnepf, E., 1973: Mikrotubulus-Anordnung und -Umordnung Wandbildung und Zellmorphogenese in jungenSphagnum-Blättchen. Protoplasma78, 145–173.
—, 1984: Pre- and postmitotic reorientation of microtubule arrays in youngSphagnum leaflets: Transitional stages and initiation sites. Protoplasma120, 100–112.
Wick, S. M., Hepler, P. K., 1980: Localization of Ca++-containing antimonate precipitates during mitosis. J. Cell Biol.86, 500–513.
—,Duniec, J., 1983: Immunofluorescence microscopy of tubulin and microtubule arrays in plant cells. I. Pre-prophase band development and concomitant appearance of nuclear envelope-associated tubulin. J. Cell Biol.97, 235–243.
— —, 1984: Immunofiuorescence microscopy of tubulin and microtubule arrays in plant cells. II. Transition between the preprophase band and the mitotic spindle. Protoplasma122, 45–55.
—,Seagull, R. W., Osborn, M., Weber, K., Gunning, B. E. S., 1981: Immunofiuorescence microscopy of organized microtubule arrays in structurally-stabilized meristematic plant cells. J. Cell Biol.89, 685–690.
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Apostolakos, P., Galatis, B. Studies on the development of the air pores and air chambers ofMarchantia paleacea . Protoplasma 128, 120–135 (1985). https://doi.org/10.1007/BF01276334
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DOI: https://doi.org/10.1007/BF01276334