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Direct regenerationin vitro and transient GUS expression inMentha xpiperita

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Abstract

Genetic transformation of peppermint is known to be very difficult essentially because of low efficiency regeneration. A regeneration protocol allowing 51% shooting frequency is proposed. Transient β-glucuronidase expression and adjustment of selection pressure with kanamycin are also reported. The final retained method to attempt peppermint transformation is:Agrobacterium inoculation or biolistic treatment of the first apical leaves ofin vitro clones, regeneration in the dark with kanamycin (1 mg l−1) and 6-benzylaminopurine (2 mg l−1), followed by selection of regenerated shoots with 200 mg 1−1 kanamycin.

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Abbreviations

BA:

6-benzylaminopurine

GUS:

β-glucuronidase

MS:

Murashige and Skoog (1962)

NAA:

α-naphthalenacetic acid

PIG:

particle inflow gun

SEM:

scanning electron microscope

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Authors and Affiliations

  1. Laboratoire de Biotechnologies Appliquées aux Plantes Aromatiques, Faculté des Sciences et Techniques, Université Jean Monnet, 23 rue du Dr Michelon, 42023, Saint-Etienne Cédex 2, France

    J. -C. Caissard, O. Faure, F. Jullien, M. Colson & A. Perrin

Authors
  1. J. -C. Caissard
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  2. O. Faure
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  3. F. Jullien
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  4. M. Colson
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  5. A. Perrin
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Caissard, J.C., Faure, O., Jullien, F. et al. Direct regenerationin vitro and transient GUS expression inMentha xpiperita . Plant Cell Reports 16, 67–70 (1996). https://doi.org/10.1007/BF01275452

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  • DOI: https://doi.org/10.1007/BF01275452

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