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Physikalische Eigenschaften des Australia-SH-Antigens (Au-SH-Antigen)

Physical properties of Australia-SH-antigen

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Zusammenfassung

Die Komplementbindungsreaktion weist ein Au-SH-Partikel nach, das sich weder in Dichte, Sedimentation, chromatographischem Verhalten, noch in seiner Morphologie von dem Partikel unterscheidet, das im Immundiffusionstest bestimmt wird. Zwischen beiden serologischen Tests besteht außerdem eine sehr gute Korrelation der Antigentiter. Die Sedimentationskonstante des Au-SH-Antigens beträgt 40 ±7 S, seine Dichte 1.16 g/cm3. Aus beiden physikalischen Konstanten berechnet sich ein mittlerer Durchmesser von 21.2 ±2 mμ. Dieser Wert entspricht der elektronenoptisch ermittelten Größe des Au-SH-Antigens. Beide Bestimmungsmethoden liefern eine Größenvariation, die sich von 14 bis 27 mμ erstreckt.

Summary

The complement fixation test detects an Australia-SH-antigen particle which physically (density, sedimentation, chromatographic behaviour on DEAE-cellulose) and morphologically is identical to that particle which is detected by immunodiffusion. Between both serological tests there is an excellent correlation of antigen titers. The sedimentation coefficient of Australia-SH-antigen is 40 ±7S, its density in sucrose 1.16 g/cm3. From these both physical constants the average diameter of Australia-SH-antigen can be calculated to 21.2 ±2 mμ, a value which corresponds to the seize of Australia-SH-antigen estimated by electron microscopy. The seize of the particles varies between 14 and 27 mμ.

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Author notes

  1. A. Schober & R. Thomssen

    Present address: Hygiene-Institut der Universität Göttingen, BRD

  2. U. Kaboth

    Present address: Medizinische Universitätsklinik Göttingen, BRD

Authors and Affiliations

  1. Hygiene-Institut der Universität Göttingen, BRD

    U. Kaboth

  2. Medizinische Universitätsklinik Göttingen, BRD

    A. Schober & R. Thomssen

Authors
  1. A. Schober
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  2. U. Kaboth
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  3. R. Thomssen
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Additional information

Prof. Dr. med. R.Haas zum 60. Geburtstag.

Die Untersuchungen wurden von der Deutschen Forschungsgemeinschaft unterstützt.

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Schober, A., Kaboth, U. & Thomssen, R. Physikalische Eigenschaften des Australia-SH-Antigens (Au-SH-Antigen). Archiv f Virusforschung 33, 364–374 (1971). https://doi.org/10.1007/BF01254693

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  • DOI: https://doi.org/10.1007/BF01254693

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