Summary
Three strains of a small, hemagglutinating DNA virus were isolated from the feces of calves and identified as bovine parvovirus type 1 (HADEN). The virus readily multiplied with cytopathic effect and produced eosinophilic intranuclear inclusion bodies in bovine kidney cell cultures. The virus agglutinated erythrocytes of horse, sheep, goat, guinea pig, hamster, duck, goose and human, but not those of mouse, rabbit, chicken and cattle. The growth of the virus was inhibited by 5-iodo-2′-deoxyuridine. The virus showed a high degree of acid- and heat-stability, and resistance to ether, chloroform, sodium deoxycholate, and trypsin, and was not affected by incubation at 50° C for 60 minutes in the presence of 1m MgCl2. The virus passed through membrane filters of 50 nm pore size. The buoyant density of the virion was estimated at 1.38 g/ml in CsCl density gradient.
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Inaba, Y., Kurogi, H., Takahashi, E. et al. Isolation and properties of bovine parvovirus type 1 from Japanese calves. Archiv f Virusforschung 42, 54–66 (1973). https://doi.org/10.1007/BF01250507
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DOI: https://doi.org/10.1007/BF01250507