Abstract
Simultaneous depletion of phenylalanine and tyrosine by phenylalanine ammonia lyase is described in a mutual competitive inhibition model. The enzymes obtained fromSporidiobolus pararoseus andRhodosporidium toruloides were charaterized in terms of stability, optimal reaction parameters and kinetic behaviour. Both enzymes followed Michaelis-Menten kinetics with respect to the two amino acids. However, the enzyme fromRhodosporidium toruloides was inhibited by high tyrosine concentrations.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Abell, C.W. & Shen, R. 1987 Phenylalanine ammonia lyase from yeastRhodotorula glutinis.Methods in Enzymology 142, 242–251.
Bezanson, G.S., Desaty, D., Emes, A.V. & Vining, L.C. 1970 Biosynthesis of cinnamamide and detection of phenylalanine ammonia lyase inStreptomyces verticillatus.Canadian Journal of Microbiology 16, 147–151.
Box, G.E.P. & Wilson, K.B. 1951 On the experimental attainment of optimum conditions.Journal of Research of the Statistics Society, Series B 13, 1–45.
Camm, E.L. & Towers, G.H.N. 1969 Phenylalanine ammonia lyase.Phytochemistry 8, 1407–1413.
Evans, C.T., Hanna, K., Conrad, D., Peterson, W. & Misawa, M. 1987a Production of phenylalanine ammonia lyase (PAL): isolation and evaluation of yeast strains suitable for commercial production ofl-phenylalanine.Applied Microbiology and Biotechnology 25, 406–414.
Evans, C.T., Hanna, K., Payne, C., Conrad, D. & Misawa, M. 1987b Biotransformation oftrans-cinnamic acid tol-Phenylalanine: optimization of reaction conditions using whole yeast cells.Enzyme and Microbial Technology 9, 417–421.
Gilbert, H.J. & Tully, M. 1985 Protection of phenylalanine ammonia lyase from proteolytic attack.Biochemical and Biophysical Research Communications 131, 557–563.
Hamilton, B.K., Hsiao, H.Y., Swan, W.E., Anderson, D.M. & Delente, J.J. 1985 Manufacture ofl-amino acids with bioreactors.Trends in Biotechnology 3, 64–68.
Havir, E.A. & Hanson, K.R. 1968l-Phenylalanine ammonia lyase. I. Purification and molecular size of the enzyme from potato tubers.Biochemistry 7, 1896–1903.
Hodgins, D.S. 1971 Yeast phenylalanine ammonia lyase: Purification, properties & the identification of catalytically essential dehydroalanine.Journal of Biological Chemistry 246, 2977–2985.
Kalghatgi, K.K. & Subba Rao, P.V. 1975 Microbiall-phenylalanine ammonia lyase. Purification, subunit structure and kinetic properties of the enzyme fromRhizoctonia solani.Biochemical Journal 149, 65–72.
Lopez-Bajonero, J., Lara, P., Galvez, M.A., Velazquez, A. & Lopez-Munguia, A. 1991 Enzymatic production of a low-phenylalanine product from skim milk powder and caseinate.Journal of Food Science 56, 938–942.
Marusich, W.C., Jensen, R.A. & Zamir, L.O. 1981 Induction ofl-phenylalanine ammonia lyase during utilization of phenylalanine as a carbon or nitrogen source inRhodotorula glutinis.Journal of Bacteriology 146, 1013–1019.
Ogata, K., Uchiyama, K., Yamada, H. & Tochikura, T. 1967 Metabolism of aromatic amino acids in microorganisms. Part II. Properties of phenylalanine ammonia lyase ofRhodotorula.Agricultural and Biological Chemistry 31, 600–606.
Orndorff, S.A., Constantino, N., Steward, D. & Durham, D.R. 1988 Strain improvement ofRhodotorula graminia for production of a novell-phenylalanine ammonia lyase.Applied and Environmental Microbiology 54, 996–1006.
Parkhurst, J.R. & Hodgins, D.S. 1971 Phenylalanine and tyrosine ammonia lyase activity inSporobolomyces pararoseus.Phytochemistry 10, 2997–3000.
Sikora, L.A. & Marzluf, G.A. 1982 Regulation ofl-phenylalanine ammonia lyase byl-phenylalanine and nitrogen inNeurospora crassa.Journal of Bacteriology 150, 1287–1291.
Wieder, K.J., Palczuk, N.C., van Es, T. & Davis, F.F. 1979 Some properties of polyethylene glycol: phenylalanine ammonia lyase adducts.Journal of Biological Chemistry 254, 12579–12587.
Yamada, S., Nabe, K., Izuo, N., Nakamichi, K. & Chibata, I. 1981 Production ofl-phenylalanine fromtrans-cinnamic acid withRhodotorula glutinis containingl-phenylalanine ammonia lyase activity.Applied and Environmental Microbiology 42, 773–778.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kawasaki Watanabe, S., Hernandez-Velazco, G., Iturbe-Chiñas, F. et al. Phenylalanine ammonia lyase fromSporidiobolus pararoseus andRhodosporidium toruloides: Application for phenylalanine and tyrosine deamination. World J Microbiol Biotechnol 8, 406–410 (1992). https://doi.org/10.1007/BF01198755
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01198755