Conclusions
-
1.
α-Chymotrypsin (CT), enclosed in positively and negatively charged polyelectroiyte gels based on methacrylate, with a preservation of high catalytic activity, was produced.
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2.
The pH profile of the enzymatic activity of CT enclosed in gel is shifted in absolute magnitude by approximately 1 pH unit, while the sign of the shift agrees with the theory.
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3.
The incorporation of CT into a negatively charged gel leads to a sharp (600-fold) increase in its stability with respect to monomolecular thermal inactivation. The stabilization is due to the influence of the polymer matrix on the protein globule of the enzyme.
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Literature cited
I. V. Berezin, A. M. Klibanov, and K. Martinek, Uspekhi Khimii,44, 17 (1975).
P. S. Bunting and K. J. Laidler, Canad. J. Biochem.,51, 1598 (1973).
K. Martinek, V. S. Gol'dmakher A. M. Klibanov, and J. V. Berezin, FEBS Letters,51, 152 (1975).
S. V. Kol'tsova, M. V. Glinka, N. G. Illarinonova, and G. V. Samsonov, Molekul. Biol.,5, 225 (1971).
I. V. Berezin, A. M. Klibanov, V. S. Gol'dmakher, and K. Martinek, Dokl. Akad. Nauk SSSR,218, 367 (1974).
H. Kaplan and K. J. Laidler, Canad. J. Chem.,45, 547 (1967).
Y. Levin, M. Pecht, L. Goldstein, and E. Katchalski, Biochemistry,3, 1913 (1964).
A. Katchalski, N. Shavit, and H. Eistenberg, J. Polymer Sci.,13, 69 (1954).
L. Goldstein, Abstracts of Post-Congress Symposium on Preparation, Properties and Application of Immobilized Enzymes, Hungarian Szeged, September 1974.
L. Goldstein, Biochemistry,11, 4072 (1972).
I. V. Berezin, Kh. Vill', K. Martinek, A. K. Yatsimirskii, and M. S. Khludova, Molekul. Biol.,1, 843 (1967).
M. L. Bender, M. L. Begue-Canton, R. L. Bleakeley, L. J. Brubacher, J. Feder, C. R. Gunter, F. J. Kezdy, J. V. Killheffer, T. H. Marshall, C. G. Miller, R. W. Roeske, and J. K. Stoops, J. Amer. Chem. Soc.,88, 5890 (1966).
G. Brown, D, Thomas, G. Gellf, D. Domurado, A. M. Berjonnean, and C. Guillon, Biotechnol. and Bioeng.,15, 359 (1973).
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Translated from Izvestiya Akademii Nauk SSSR, Seriya Khimicheskaya, No. 8, pp. 1764–1768, August, 1975.
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Chernysheva, A.V., Marinek, K., Klibanov, A.M. et al. Catalytic properties and thermal stability ofα-chymotrypsin in polyelectrolyte gels based on methacrylate. Russ Chem Bull 24, 1648–1652 (1975). https://doi.org/10.1007/BF01100013
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DOI: https://doi.org/10.1007/BF01100013