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Solid phase synthesis of peptides with polyethylene glycol-modified protease in organic solvents

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Summary

Trypsin, chymotrypsin, papain, thermolysin and pepsin were modified with a polyethylene glycol derivative (PEG). Each PEG-protease became soluble and active in organic solvents and catalyzed peptide bond formation with their substrate specificity. Using five kinds of PEG-proteases, various kinds of tripeptides were synthesized by solid phase system in methanol or in dimethylformamide.

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Abbreviations

Boc:

t-butyloxycarbonyl

Z:

benzyloxycarbonyl

Fmoc:

9-fluorenylmethyloxycarbonyl

PAM:

4-(oxymethyl)phenylacetoamidomethyl

HMP:

4-(oxymethyl)phenoxymethyl.

References

  1. Merrifield, R.B. (1963) in “The Peptides: Analysis, Synthesis, Biology”, vol.2 (Gross, E. and Meienhofer, J., eds.) pp.1–284, Academic Press, New York.

    Google Scholar 

  2. Yajima, H. and Fujii, N. (1981) in “Chemical Synthesis and Sequencing of Peptides and Proteins” (Liu, T.Y., Schecher, A., Heinrikson, R. and Condliff, P., eds.) pp.21, Elsevier, North-Holland.

    Google Scholar 

  3. Blake, J. (1986) Int. J. Peptide Protein 27, 191–200.

    Google Scholar 

  4. Inada, Y., Takahashi, K., Yoshimoto, T., Ajima, A., Matsushima, A. and Saito, Y. (1986) Trends Biotechnol. 4, 190–194.

    Google Scholar 

  5. Takahashi, K., Ajima, A., Yoshimoto T. and Inada, Y. (1984) Biochem. Biophys. Res. Commun. 125, 761–766.

    PubMed  Google Scholar 

  6. Lee, H., Takahashi, K., Kodera, Y., Ohwada, K., Tsuzuki, T., Matsushima, A. and Inada, Y. (1988) Biotechnol. Lett. 5, 403–407.

    Google Scholar 

  7. Matsushima, A., Okada, M. and Inada, Y. (1984) FEBS Lett. 178, 275–277.

    PubMed  Google Scholar 

  8. Layne, E. (1957) in “Methods Enzymol.”, vol.3, pp.450–451.

  9. Habeeb, A.F.S.A. (1966) Anal. Biochem. 14, 328–336.

    PubMed  Google Scholar 

  10. Kaiser, E., Colescott, R.L., Bossinger, C.D. and Cook, P.I. (1970) Anal. Biochem. 34, 595.

    PubMed  Google Scholar 

  11. Pettit, G.R. (1980 and 1982) in “Synthetic Peptides”, vol. 5 and 6. Elsevier, N.Y.

    Google Scholar 

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Authors and Affiliations

  1. Seikagaku Kogyo Co., Ltd., 3-1253, Tateno, 189, Higashiyamato, Tokyo, Japan

    K. Sakurai & K. Kashimoto

  2. Department of Materials Science and Technology, Toin University of Yokohama, 1614, Kurogane-cho, Midori-ku, 227, Yokohama, Japan

    Y. Kodera & Y. Inada

Authors
  1. K. Sakurai
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  2. K. Kashimoto
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  3. Y. Kodera
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  4. Y. Inada
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Sakurai, K., Kashimoto, K., Kodera, Y. et al. Solid phase synthesis of peptides with polyethylene glycol-modified protease in organic solvents. Biotechnol Lett 12, 685–688 (1990). https://doi.org/10.1007/BF01088194

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  • DOI: https://doi.org/10.1007/BF01088194

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