Skip to main content
SpringerLink
Log in

Detection of Eber-1 RNA in primary brain lymphomas in immunocompetent and immunocompromised patients

  • Published:
Journal of Neuro-Oncology Aims and scope Submit manuscript

Summary

Tissue from primary central nervous system lymphoma (PCNSL) which developed in five patients with acquired immuno deficiency syndrome (AIDS), nine patients without immunodeficiency, and two Epstein-Barr virus (EBV)-positive control cell lines (B95-8 and Raji) were examined for the presence of EBER-1 RNA. The tissues were hybridized with digoxigenin-labeled sense or anti-sense EBER-1 riboprobes. In all five AIDS-related PCNSLs, strong hybridization signals were found with the EBER-1 anti-sense probe. Signals could be eliminated by preincubation of the tissues with RNase-A. Hybridization with the EBER-1 sense probe showed no signal. All PCNSLs from immunocompetent patients (five paraffin-embedded, four frozen) showed no hybridization signals with EBER-1 sense or antisense probe but good hybridization signals with probes to immunoglobulin kappa or lambda light chain indicating RNA preservation. The paraffin-embedded B95-8-positive control cell-line showed positive hybridization in most cells with the anti-sense EBER-1 probe, and up to one percent of the cells had a weak signal with the sense probe. Most Raji cells showed a uniform signal with the anti-sense EBER-1 probe only. We conclude that, PCNSLs that arise in AIDS patients are associated with latent EBV infections, whereas PCNSLs from immunocompetent patients are not indicating a probable role for EBV in pathogenesis of these tumors.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. So YT, Beckstead JH, Davis RL: Primary central nervous system lymphoma in acquired immune deficiency syndrome: A clinical and pathological study. Ann Neurol 20: 566–574, 1986

    PubMed  Google Scholar 

  2. Eby NL, Grufferman S, Flannelly CMet al.: Increasing incidence of primary brain lymphoma in the US. Cancer 62: 2461–2465, 1988

    PubMed  Google Scholar 

  3. Levine A: Acquired immunodeficiency syndrome-related lymphoma. Blood 80: 8–20, 1992

    PubMed  Google Scholar 

  4. Rosenberg NL, Hochberg FH, Miller G, Kleinschmidt-De-Masters BK: Primary central nervous system lymphoma related to Epstein-Barr virus in a patient with acquired immune deficiency syndrome. Ann Neurol 20: 98–102, 1986

    PubMed  Google Scholar 

  5. Bashir R, Harris N, Hochberg Fet al.: Detection of Epstein-Barr virus in primary CNS lymphoma in immunocompromised patients. Neurol 39: 813–817, 1989

    Google Scholar 

  6. Borisch-Chappnis B, Nezelofc, Muller Het al.: Different Epstein-Barr virus expression in lymphomas from immunocompromised and immunocompetent patients. Am J Pathol 136: 751–758, 1990

    PubMed  Google Scholar 

  7. Rouah E, Rogers BB, Wilson DRet al.: Demonstration of Epstein-Barr virus in primary central nervous system lymphomas by the polymerase chain reaction andin situ hybridization. Human Path 21: 545–550, 1990

    Google Scholar 

  8. Nakhleh RE, Manivel JC, Copenhaver CM, Sung JH, Strickler JG:In situ hybridization for the detection of Epstein-Barr virus in central nervous system lymphomas. Cancer 67: 444–448, 1991

    PubMed  Google Scholar 

  9. Morgello S: Epstein-Barr and human immunodeficiency viruses in acquired immunodeficiency syndrome-related primary central nervous system lymphoma. Amer J Path 141: 441–450, 1992

    PubMed  Google Scholar 

  10. Ambinder R, Lambe B, Mann Ret al: Oligonucleotides for polymerase chain reaction amplification and hybridization detection of Epstein-Barr virus DNA in clinical specimens. Mol Cell Probes 4: 397–407, 1990

    PubMed  Google Scholar 

  11. DeAngelis LM, Wong E, Rosenblum M, Furneaux H: Epstein-Barr virus in acquired immune deficiency syndrome (AIDS) and non-AIDS primary central nervous system lymphoma. Cancer 70: 1607–1611, 1992

    PubMed  Google Scholar 

  12. Bignon YJ, Clavelou P, Ramos Fet al.: Detection of Epstein-Barr viral sequences by genomic amplification in primary lymphoma cells from 10 patients without immunodeficiency. Neurology 41: 1152–1153, 1991

    PubMed  Google Scholar 

  13. Howe JG, Steitz JA: Localization of Epstein-Barr virus-encoded small RNAs byin situ hybridization. Proc Natl Acad Sci USA 83: 9006–9010, 1986

    PubMed  Google Scholar 

  14. Glickman JN, Howe JG, Steitz JA: Structural analyses of EBER1 and EBER2 ribonucleoprotein particles present in Epstein-Barr virus-infected cells. J Virol 62: 902–911, 1988

    PubMed  Google Scholar 

  15. MacMahon EME, Glass JD, Hayward SDet al.: Epstein-Barr virus in AIDS-related primary central nervous system lymphoma. Lancet 338: 969–973, 1991

    PubMed  Google Scholar 

  16. Chang KL, Flaris N, Hickey WF, Johnson RM, Meyer JS, Weiss LM: Brain lymphomas of immunocompetent and immunocompromised patients: study of the association with Epstein-Barr virus. Modern Path 6: 427–432, 1993

    Google Scholar 

  17. Bashir RB, Hochberg F, Singer RH: Detection of Epstein-Barr virus byin-situ hybridization. Progress toward development of a non-isotopic diagnostic test. Am J Pathol 135: 1035–1044, 1989

    PubMed  Google Scholar 

  18. Bashir RM, Harris NL, Hochberg FH, Singer RM: Detection of Epstein-Barr virus in CNS lymphomas byin situ hybridization. Neurology 39: 813–817, 1989

    PubMed  Google Scholar 

  19. Non-Hodgkin's lymphoma pathologic classification project: National Cancer Institute sponsored study of classifications of non-Hodgkin's lymphomas: Summary and of a working formulation for clinical usage. Cancer 49: 2115, 1982

  20. Bashir R, Coakham H, Hochberg F: Expression of LFA-1/ICAM-1 in CNS lymphomas: possible mechanism for lymphoma homing into the brain. J Neuro-Oncology 12: 103–110, 1992

    Google Scholar 

  21. Bashir R, Freedman A, Harris N, Bain K, Nadler L, Hochberg F: Immunophenotypic profile of CNS lymphoma: a review of eighteen cases. J Neuro-Oncology 7: 249–254, 1989

    Google Scholar 

  22. Vroman B, Luka J, Rodriguez M, Pearson G: Characterization of a major protein with a molecular weight of 160,000 associated with the viral capsid antigen of Epstein-Barr virus. J Virol 53: 107–112, 1985

    PubMed  Google Scholar 

  23. Hamilton-Dutoit SJ, Polksen G, Franzmann MBet al.: AIDS-related lymphoma: Histopathology, immunophenotype, and association with EBV as demonstrated byin situ nucleic acid hybridization. Am J Pathol 138: 149–154, 1991

    PubMed  Google Scholar 

  24. Meeker TC, Shiramizu B, Kaplan Let al.: Evidence of molecular subtypes of HIV-associated lymphoma: Division into peripheral monoclonal, polyclonal and central nervous system lymphoma. AIDS 5: 669–675, 1991

    PubMed  Google Scholar 

  25. Medawar PB: Immunity to homologous grafted skin: II. The fate of skin homografts transplanted to the brain, to subcutaneous tissue and to the anterior chamber of the eye. Br J Exp Pathol 29: 58–64, 1948

    Google Scholar 

  26. Ljunggren H, Yamasaki T, Collins P, Klein G, Karre K: Selective acceptance of MHC Class I-deficient tumor grafts in the brain. J Exp Med 167: 730–735, 1988

    PubMed  Google Scholar 

  27. Wekerle H, Linington C, Lassmann H, Meyermann R: Cellular immune reactivity within the CNS. TINS 9: 271–277, 1986

    Google Scholar 

  28. Nilsson K, Giovanella BC, Stehlin JS, Klein G: Tumorigenicity of human hematopoietic cell lines in athymic nude mice. Int J Cancer 19: 337–342, 1977

    PubMed  Google Scholar 

  29. Bashir RM, Masih A, Kallweit Ket al.: Evolution of clonality and invasive behavior of Epstein-Barr virus immortalized lymphoblastoid cell lines in SCID mice brains. Lab Invest 67: 450–456, 1992

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Internal Medicine, University of Nebraska Medical Center, Omaha, NE

    R. Bashir

  2. Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE

    B. McManus, C. Cunningham & D. Weisenburger

  3. Department of Neurology, Massachusetts General Hospital, Boston, MA, USA

    F. Hochberg

Authors
  1. R. Bashir
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B. McManus
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C. Cunningham
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. D. Weisenburger
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. F. Hochberg
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bashir, R., McManus, B., Cunningham, C. et al. Detection of Eber-1 RNA in primary brain lymphomas in immunocompetent and immunocompromised patients. J Neuro-Oncol 20, 47–53 (1994). https://doi.org/10.1007/BF01057960

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01057960

Key words

Search

Navigation