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Batch and fed batch cultivations for the temperature induced production of a recombinant protein inEscherichia coli

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Summary

The temperature induced intracellular production of the fused protein SpA-βgal (protein A/β-galactosidase) withE. coli was compared in batch and fed batch culture. By introducing fed batch cultivation a final cell dry weight of 77.0 g/l was achieved, as compared to 16.4 g/l in batch cultivation. The concentration of SpA-βgal in the fed batch cultivation was very high, 19.2 g/l. This corresponded to 25% of the cell dry weight.

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Authors and Affiliations

  1. Department of Biochemistry and Biotechnology, Royal Institute of Technology, S-100 44, Stockholm, Sweden

    Lars Strandberg & Sven-Olof Enfors

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  1. Lars Strandberg
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  2. Sven-Olof Enfors
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Strandberg, L., Enfors, SO. Batch and fed batch cultivations for the temperature induced production of a recombinant protein inEscherichia coli . Biotechnol Lett 13, 609–614 (1991). https://doi.org/10.1007/BF01033419

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