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Stabilisation of a recombinant yeast plasmid in nonselective medium by cyclic growth rate changes

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Summary

The 2μm based yeast plasmid, pLG669-z, was stably maintained in aS. cerevisiae strain during continuous culture with cyclic growth rate changes. In the absence of a selective mechanism, the fraction of plasmid containing cells remained high, and even without optimisation, high gene expression levels could be obtained.

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References

  • Caunt, P., Impoolsup, A. and Greenfield P.F. (1989) Manuscript in preparation.

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Authors and Affiliations

  1. Centre for Molecular Biology and Biotechnology, University of Queensland, 4067, St. Lucia, Queensland, Australia

    A. Impoolsup, P. Caunt & P. F. Greenfield

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  1. A. Impoolsup
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  2. P. Caunt
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  3. P. F. Greenfield
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Impoolsup, A., Caunt, P. & Greenfield, P.F. Stabilisation of a recombinant yeast plasmid in nonselective medium by cyclic growth rate changes. Biotechnol Lett 11, 605–608 (1989). https://doi.org/10.1007/BF01025266

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  • DOI: https://doi.org/10.1007/BF01025266

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