Abstract
The allelopathic nature ofJ. nigra L. was investigated in several planted mixed hardwood stands located near Syracuse, New York. Concentrations of chloroform-extracted juglone from soil collected beneathJ. nigra was determined by thin-layer chromatography (TLC) and high-pressure liquid chromatography (HPLC). Soil juglone concentrations were corrected based on recovery of synthetic juglone added to soil. Soil juglone levels were high in the spring, decreased during the summer, and were high again in the fall. The quantification of juglone from soil by HPLC was found to be more accurate than by TLC. Regression analysis indicated that individual tree variation in soil juglone levels could not be explained by differences in soil moisture, pH, organic matter content, and texture. The results of juglone recovery experiments suggest that chloroform-extractable juglone does not persist in soil. Juglone degradation by microorganisms could only explain a portion of the juglone decline. Ordinations revealed that the herbaceous and woody vegetation beneathJ. nigra, in comparison to vegetation beneathAcer saccharum andQuercus rubra, is distinct in only one of the four stands studied. This vegetational difference did not appear to be a consequence of any strong allelopathic influences ofJ. nigra (Scheffe's method of contrast, chi-square analysis). The allelopathic nature of juglone under these field conditions is questionable.
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Al-Naib, F.A.G., andRice, E.L. 1971. Allelopathic effects ofPlatanus occidentalis. Bull.Torrey Bot. Club 98:75–82.
Beals, E.W. 1984. Bray-Curtis ordination: An effective strategy for analysis of multivariate ecological data.Adv. Ecol. Res. 14:1–55.
Beatty, S.W. 1984. Influence of microtopography and canopy species on spatial patterns of forest understory plants.Ecology 65:1406–1419.
Biswell, H.H. 1935. Effects of environment upon the root habits of certain deciduous forest trees.Bot. Gaz. 96:676–708.
Boes, T.K. 1986. Allelopathy: Chemical interactions between plants.Am. Nurs. 163:67–72.
Borner, H. 1960. Liberation of organic substances from higher plants and their role in the soil sickness problems.Bot. Rev. 26:393–424.
Bratton, G.F. 1974. The role and allelopathic effects ofJuglans nigra in old field succession. MS thesis. Emporia Kansas State College, Emporia, Kansas.
Bratton, S.P. 1976. Resource division in an understory herb community: Responses to temporal and microtopographic gradients.Am. Nat. 110:679–693.
Brooks, M.G. 1951. Effects of black walnut trees and their products on other vegetation. Agricultural Extension State Bulletin No. 347, West Virginia University, Morgantown.
Camp, R.F. 1986. Walnuts and white pine can be grown together successfully.Tree Plant. Notes U.S. Dept. Agric. For. Serv. 37:29–31.
Chou, C.H., andKuo, Y.L. 1986. Allelopathic research of subtropical vegetation in Taiwan III. Allelopathic exclusion of understory byLeucaena leucocephala (Lam) de Wit.J. Chem. Ecol. 12:1431–1448.
Chou, C.H., andYang, C.M. 1982. Allelopathic research of subtropical vegetation in Taiwan II. Comparative exclusion of understory byPhyllostachys edulis andCryptomeria japonica.J. Chem. Ecol. 8:1489–1507.
Cline, S., andNeely, D. 1984. Relationship between juvenile-leaf resistance to anthracnose and the presence of juglone and hydrojuglone glucoside in black walnut.Phytopathology 74:185–188.
Coder, K.D. 1983. Seasonal changes of juglone potential in leaves of black walnut (Juglans nigra L.).J. Chem. Ecol. 8:1203–1212.
Collins, B.S., Ferrara, L.S., andMotto, H.L. 1984. Coincidence of spring herb distribution and flowering with tree bases in a New Jersey Piedmont forest.Bull. Torrey Bot. Club 111:301–306.
Collins, S.L., andGood, R.E. 1986. Canopy-ground layer relationships of oak-pine forests in the New Jersey Pine Barrens.Am. Midl. Natl. 117:280–288.
Cook, M.T. 1921. Wilting caused by walnut trees.Phytopathology 11:346.
Crozier, C.R., andBoerner, R.E. 1984. Correlations of understory herb distribution patterns with microhabitats under different tree species in a mixed mesophytic forest.Oecologia (Berl.) 62:337–343.
Crozier, C.R., andBoerner, R.E. 1986. Stemflow induced soil nutrient heterogeneity in a mixed mesophytic forest.Bartonia 52:1–8.
Daglish, C. 1950. The determination and occurrence of a hydrojuglone glucoside in the walnut.Biochem. J. 47:458–462.
Dalton, B.R., Blum, U., andWeed, S.B. 1983. Allelopathic substances in ecosystems: Effectiveness of sterile soil components in altering recovery of ferulic acid.J. Chem. Ecol. 9:1185–1201.
Davidson, J. 1939. Some unanswered questions.North. Nut Grow. Assoc. Ann. Rep. 30:58–66.
Davis, E.F. 1928. The toxic principle ofJuglans nigra as identified with synthetic juglone, and its toxic effects on tomato and alfalfa plants.Am. J. Bot. 15: 620.
Dawson, J.O., andSeymour, P.E. 1983. Effects of juglone concentration on growth in vitro ofFrankia ArI3 andRhizobium japonicum strain 71.J. Chem. Ecol. 9:1175–1183.
del Moral, R., andMuller, C.H. 1970. The allelopathic effects ofEucalyptus camaldulensis.Am. Midl. Nat. 83:254–282.
De Scisciolo, B. 1988. The seasonal patterns of juglone in soil beneathJuglans nigra (black walnut) and the influence ofJ. nigra on understory vegetation. MS thesis. State University of New York College of Environmental Science and Forestry, Syracuse, New York.
Everett, R.L., Sharrow, S.H., andMeeuwig, R.O. 1983. Pinyon-juniper woodland understory distribution patterns and species associations.Bull. Torrey Bot. Club 110:454–463.
Fisher, R.F. 1978. Juglone inhibits pine growth under certain moisture regimes.Soil Sci. Soc. Am. J. 42:801–803.
Friedham, E.A.H. 1934. Natural reversible oxidation-reduction systems as accessory catalysts in respiration: juglon and lawson.Biochem. J. 28:180–188.
Fuerst, E.P., andPutnam, A.R. 1983. Separating the competitive and allelopathic components of interference: theoretical principles.J. Chem. Ecol. 9:937–944.
Funk, D.T., Case, P.J., Rietveld, W.J., andPhares, R.E. 1979. Effects of juglone on the growth of coniferous seedlings.For. Sci. 25:452–454.
Gabriel, W.J. 1975. Allelopathic effects of black walnut on white birches.J. For. 73:234–237.
Gersper, P.L., andHolowaychuk, N. 1970. Effects of stemflow water on a Miami soil under a beech tree: II Chemical properties.Soil Sci. Soc. Am. Proc. 34:786–794.
Gersper, P.L., andHolowaychuk, N. 1971. Some effects of stem flow from forest canopy trees on chemical properties of soils.Ecology 52:691–702.
Gleason, H.A., andCronquist, A. 1963. Manual of vascular plants of northeastern United States and adjacent Canada. D. Van Nostrand, New York.
Graves, C.H., Hedin, P.A., andLanghans, V.E. 1979. A survey of juglone levels among pecan, hickory, and walnut, pp. 103–111,in: Proc. 72nd Annu. Conv. Southeast Pecan Growers Assoc., Hilton Head, South Carolina.
Greene, K.W. 1930. The toxic (?) effect of the black walnut.North. Nut Grow. Assoc. Proc. 1929:152–157.
Gries, G.A. 1943. Juglone—the active agent in walnut toxicity.North. Nut Grow. Assoc. Annu. Rep. 34:52–55.
Hedin, P.A., Langhans, V.E., andGraves, C.H. 1979. Identification of juglone in pecan as a possible factor of resistance toFusicladium effusum.J. Agric. Food Chem. 27:92–94.
Hedin, P.A., Collum, D.H., Langhans, V.E., andGraves, C.H. 1980. Distribution of juglone and related compounds in pecan and their effect onFusicladium effusum.J. Agric. Food Chem. 28:340–342.
Hicks, D.J. 1980. Intrastand distribution patterns of southern Appalachian cove forest herbaceous species.Am. Midl. Nat. 104:209–223.
Holch, A.E. 1931. Development of roots and shoots of certain deciduous tree seedlings indifferent forest sites.Ecology 12:259–298.
Kershaw, K.A., andLooney, J.H.H. 1985. Quantitative and Dynamic Plant Ecology, 3rd ed. Edward Arnold, London.
Lee, K.C., andCampbell, R.W. 1969. Nature and occurrence of juglone inJuglans nigra L. Hortic. Sci. 4: 297–298.
Lodhi, M.A.K. 1976. Role of allelopathy as expressed by dominating trees in a lowland forest in controlling the productivity and pattern of herbaceous growth.Am. J. Bot. 63:1–8.
Lodhi, M.A.K. 1977. The influence and comparison of individual forest trees on soil properties and possible inhibition of nitrification due to intact vegetation.Am. J. Bot. 64:260–264.
Lodhi, M.A.K., andRice, E.L. 1971. Allelopathic effects ofCeltis laevigata.Bull. Torrey Bot. Club 98:83–89.
MaCuaniels, L.H., andMuenscher, W.C. 1941. Black walnut toxicity.North. Nut Grow. Assoc. Annu. Rep. 31:172–179.
MaCdaniels, L.H., andPinnow, D.L. 1976. Walnut toxicity, an unsolved problem.North. Nut Grow. Assoc. Annu. Rep. 67:114–122.
Maguire, D.A., andForman, R.T.T. 1983. Herb cover effects on tree seedling patterns in a mature hemlock-hardwood forest.Ecology 64:1367–1380.
Marking, L.L. 1970. Juglone (5-hydroxy-1,4-naphthoquinone) as a fish toxicant.Trans. Am. Fish Soc. 99:510–514.
Massey, A.B. 1925. Antagonism of the walnuts (Juglans nigra L. andJ. cinerea L.) in certain plant associations.Phytopathology 15:773–784.
Mattoon, H.G. 1944. A commercial black walnut venture.North. Nut Grow. Assoc. Annu. Rep. 35:79–82.
McCune, B. 1987. Multivariate Analysis on the PC-ORD system. A Software Documentation Report. Holcomb Research Institute, Butler University, Indianapolis, Indiana.
Oleszek, W., andJurzysta, M. 1987. The allelopathic potential of alfalfa root medicagenic acid glycosides and their fate in soil environments.Plant Soil 98:67–80.
Perry, S.F. 1967. Inhibition of respiration by juglone inPhaseolus andLycopersicon.Bull. Torrey Bot. Club 94:26–30.
Pirone, P.P. 1938. The detrimental effect of walnut to rhododendrons and other ornamentals.Nurs. Dis. Notes 11:13–16.
Ponder, F., Jr. 1987. Allelopathic interference of black walnut trees with nitrogen-fixing plants in mixed plantings, pp. 195–204,in G.R. Waller (ed.). Allelochemicals: Role in Forestry and Agriculture. ACS Symposium Series No. 330. American Chemical Society, Washington, D.C.
Ponder, F., Jr., andTadros, S.H. 1985. Juglone concentration in soil beneath black walnut interplanted with nitrogen-fixing species.J. Chem. Ecol. 11:937–942.
Prichard, R.P. 1941. Report on experimental planting Syracuse Forest Experiment Station. Bull. NYS College of Forestry Tech. Publ.57, Syracuse, New York. 43 pp.
Reinking, O.A. 1943. Possible black walnut toxicity on tomato and cabbage.North. Nut Grow. Assoc. Annu. Rep. 34:56–58.
Rettenmaier, H.J. 1985. The enzymatic bacterial decomposition of juglone. PhD thesis. University of Hohenheim, Hohenheim, West Germany. 88 pp.
Rettenmaier, H.J., Kupas, E., andLingens, F. 1983. Degradation of juglone byPseudomonas putida J 1.FEMS Microbiol. Lett. 19:193–195.
Rice, E.L. 1984. Allelopathy, 2nd ed. Academic Press, Orlando, Florida.
Richards, L.A. 1969. Diagnosis and improvement of saline and alkali soils. Agric. Handb. No. 60, USDA. U.S. Government Printing Office, Washington, D.C.
Rietveld, W.J. 1981. The significance of allelopathy in black walnut cultural systems.North. Nut Grow. Assoc. Annu. Rep. 72:117–134.
Rietveld, W.J. 1983. Allelopathic effects of juglone on germination and growth of several herbaceous and woody species.J. Chem. Ecol. 9:295–308.
Rietveld, W.J., Schlesinger, R.C., andKessler, K.J. 1983. Allelopathic effects of black walnut on European black alder coplanted as a nurse species.J. Chem. Ecol. 9:1119–1133.
Riha, S.J., James, B.R., Senesac, G.P., andPallant, E. 1986. Spatial variability of soil pH and organic matter in forest plantations.Soil Sci. Soc. Am. J. 50:1347–1352.
Schneiderhan, F.J. 1927. The black walnut (Juglans nigra L.) as a cause of the death of apple trees.Phytopathology 17:529–540.
Schreiner, E.J. 1949. Can black walnut trees poison pines?Penn. Univ. Morris Arboretum Bull. 4:94–96.
Sherman, R.R. 1971. An ecological evaluation of the allelopathic influence ofJugions nigra onLycopersicon esculentum. MS thesis. Michigan State University, East Lansing, Michigan.
Smith, R.M. 1942. Some effects of black locusts and black walnuts on southeastern Ohio pastures.Soil Sci. 53:385–398.
Sokal, R.R., andRohlf, F.J. 1981. Biometry, 2nd ed. W.H. Freeman, San Francisco.
Strong, M.C. 1944. Walnut wilt of tomato.Mich. Agric. Exp. Sta. Q. Bull. 26:194–195.
Thomson, R.H. 1971. Naturally Occurring Quinones. Academic Press. New York. pp. 2–3, 13–21.
Tukey, H.B. 1966. Leaching of metabolites from above-ground parts and its implications.Bull. Torrey Bot. Club 93:385–401.
Turner, D.P., andFranz, E.H. 1986. The influence of canopy dominants on understory vegetation patterns in an old-growth cedar-hemlock forest.Am. Midi. Nat. 116:387–393.
U.S. Department of Agriculture Soil Conservation Service. 1972. Soil survey of Onondaga County.
Voigt, O.K. 1960. Distribution of rainfall under forest stands.For. Sei. 6:2–10.
von Althen, F.W. 1968. Incompatibility of black walnut and red pine.Can. For. Serv. Bi-momh. Res. Notes 24:19.
Werner, P.A., andHarbeck, A.L. 1982. The pattern of tree seedling establishment relative to staghorn sumac cover in Michigan old fields.Am. Midi. Nat. 108:124–132.
Wiant, H.V., Jr., andRamirez, M.A. 1974. Don't plant white pine near walnut!Tree Plant Notes U.S. Dept. Agric. For. Serv. 25:30.
Wilde, S.A., Voigt, G.K., andIyer, J.G. 1972. Soil and Plant Analysis for Tree Culture. Oxford and IBH Publishing, New Delhi, India.
Wilde, S.A., Corey, R.B., Iyer, J.G., andVoigt, G.K. 1979. Soil and Plant Analysis for Tree Culture. Oxford and IBH Publishing, New Delhi, India.
Willis, R.J. 1985. The historical bases of the concept of allelopathy.J. History Biol. 18:71–102.
Wonnacott, R.J., andWonnacott, T.H. 1985. Introductory Statistics, 4th ed. John Wiley & Sons, New York.
Yen, C.P.,Pham, C.H.,Cox, G.S., andGarrett, H.E. 1978. Soil depth and root development patterns of Missouri black walnut and certain Taiwan hardwoods, pp. 36–42,in Proc. of Symp. of Root Form of Planted Trees, Victoria, B.C., Canada, May 16–19.
Zinke, P.J. 1962. The pattern of influence of individual forest trees on soil properties.Ecology 43:130–133.
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de Scisciolo, B., Leopold, D.J. & Walton, D.C. Seasonal patterns of juglone in soil beneathJuglans nigra (black walnut) and influence ofJ. nigra on understory vegetation. J Chem Ecol 16, 1111–1130 (1990). https://doi.org/10.1007/BF01021015
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DOI: https://doi.org/10.1007/BF01021015