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All you wanted to know about SELEX

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Abstract

In vitro selection, or SELEX, is a technique that allows the simultaneous screening of highly diverse pools of different RNA or DNA (dsDNA or ssDNA) molecules for a particular feature. Different examples from a great variety of applications ofin vitro selection experiments are described and a detailed overview of the method and its variations will be given. Some especially conclusivein vitro selection experiments are discussed in detail to illustrate the potential power and diversity of this method. Potential restrictions of the methods and possible ways to overcome them are pointed out.

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Authors and Affiliations

  1. Institut für Molekulare Biochemie, Genzentrum der Ludwig-Maximilian-Universität München, Würmtalstr. 221, 81375, München, Germany

    Stefanie J. Klug & Michael Famulok

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  1. Stefanie J. Klug
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  2. Michael Famulok
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Klug, S.J., Famulok, M. All you wanted to know about SELEX. Mol Biol Rep 20, 97–107 (1994). https://doi.org/10.1007/BF00996358

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  • DOI: https://doi.org/10.1007/BF00996358

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