Summary
Frozen sections cut from 14 samples of invasive carcinomas of the skin were stained with Amido black for protein determination and with dihydroxydinaphthyldisulphide fast blue to quantify reactive protein thiols (PSHr) and were then analysed microphotometrically. It was found that all of the samples exhibited significant reductions in protein levels (49%–74%) and PSHr levels (32%–53%) as compared to normal epidermis. Thus, the content of proteins of PSHr groups was 1.7 times greater in the malignant tissue examined than in normal epidermis.
These results are in accordance with those previously obtained in basal-cell epitheliomas.
References
Bajardi F, Benedetto C, Nöhammer G, Schauenstein E, Slater TF (1983) Histophotometrical investigations on the content of protein and protein thiols in the epithelium and stroma of the human uterine cervix. II. Intraepithelial neoplasia. Histochemistry 78:95–100
Katholnig E (1986) Untersuchungen über den Gehalt der normalen Epidermis und epidermaler Neoplasien an Proteinen und Proteinthiolen. Doctoral thesis, University Graz, Austria
Nöhammer G (1982) Quantitative microspectrometrical determination of protein thiols and disulfides with 2,2′-dihydroxy-6,6′-dinaphthyldisulfide (DDD). The variety of DDD-staining methods demonstrated on Ehrlich ascites tumor cells. Histochemistry 75:219–250
Nöhammer G (1984) A modification of the Amidoblack-TCA-staining for quantitative microspectrometrical determination of proteins in tissue sections. Histochemistry 80:395–400
Schauenstein E, Desoye G, Nöhammer G (1980) Quantitative Proteinbestimmung in Einzelzellen mit Amidschwarz. Histochemistry 68:75–90
Schauenstein E, Nöhammer G, Rauch HJ, Kresbach H (1985) Significant decreases in the intensity of staining for proteins and protein thiols in basal-cell epitheliomas (basaliomas) as compared to normal skin. Histochemistry 83:1–4
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nöhammer, G., Schauenstein, E., Soyer, H.W. et al. Histophotometric quantification of protein and reactive proteinthiols in invasive carcinomas of the skin. Histochemistry 85, 437–438 (1986). https://doi.org/10.1007/BF00982675
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00982675